Genotypic characterization of Bordetella bronchiseptica strains isolated from stray and pet dogs

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dc.contributor.authorSayın, Zafer
dc.contributor.authorSakmano?lu, Aslı
dc.contributor.authorErganiş, Osman
dc.contributor.authorUçan, Uçkun Sait
dc.contributor.authorHadımlı, Hasan Hüseyin
dc.contributor.authorAras, Zeki
dc.contributor.authorSanioglu, Gökçenur
dc.contributor.authorCoşkun, Alparslan Aslan
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T08:22:00Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T08:22:00Z
dc.date.issued2016
dc.departmentVeteriner Fakültesi
dc.description.abstractBordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. © 2016 PVJ. All rights reserved.
dc.identifier.endpage486en_US
dc.identifier.issn0253-8318
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage482en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/2428
dc.identifier.volume36en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUniversity of Agriculture
dc.relation.ispartofPakistan Veterinary Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBordetella Bronchiseptica
dc.subjectDermonecrotic Toxin
dc.subjectFilamentous Hemagglutinin
dc.subjectPertactin
dc.subjectRAPD-PCR
dc.titleGenotypic characterization of Bordetella bronchiseptica strains isolated from stray and pet dogs
dc.typeArticle

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