Genetic diversity and geographic variation of chromosomal races of Nannospalax xanthodon (Nordmann, 1840) and Nannospalax ehrenbergi (Nehring, 1898) from Turkey, revealed by RAPD analysis
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
The level of genetic polymorphism in chromosomal races of Nannospalax xanthodon and Nannospalax ehrenbergi was determined by applying random amplified polymorphic DNA (RAPDs) analysis. One hundred and fifty four mole rat samples belonging to two species N. xanthodon (61 populations) and N. ehrenbergi (3 populations) distributed in Anatolia were studied. Remarkable variations of diploid chromosome numbers were identified for N. xanthodon (2n=36 - 60). Eleven RAPD-PCR primers generated 160 polymorphic loci. The mean proportion of polymorphic loci was 92% (147 bands) for all chromosomal race implying high levels of genetic variability in N. xanthodon and N. ehrenbergi. Estimation of genetic diversity based on PCR amplification of RAPDs was high for both species. Results of RAPD- PCR observed within and among species were also connected with the peripatric mode of speciation. We determined that RAPD bands showed high diagnostic value between chromosomal races as they were very distinctive for each chromosomal race and absent from other forms. Genetic distance (Ds) values between chromosomal races suggest that most populations analyzed in this study may be valid biological species.
Description
Keywords
Chromosomal Race, Geographic Variation, Nannospalax Ehrenbergi, Nannospalax Santhodon, RAPD-PCR
Journal or Series
Acta Zoologica Bulgarica
WoS Q Value
N/A
Scopus Q Value
Q3
Volume
65
Issue
1
