The cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings

Basit Öğe Kaydı
dc.contributor.authorYeni, D.
dc.contributor.authorGülhan, Mehmet Fuat
dc.contributor.authorİnanç, M. E.
dc.contributor.authorAvdatek, F.
dc.contributor.authorGüngör, S.
dc.contributor.authorTürkmen, R.
dc.contributor.authorTuncer, P. B.
dc.contributor.authorTasdemir, U.
dc.date.accessioned2022-05-24T04:51:45Z
dc.date.available2022-05-24T04:51:45Z
dc.date.issued2022
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstractIn this study, the cryoprotective effect of different doses of propolis (P) on bull semen, which has solid pharmacological properties thanks to its rich phenolic components, was investigated biochemically and physiologically. Semen samples were collected from Simmental breed bulls via the artificial vagina and pooled. After dividing into five groups, control (C: no additive) and four different P (200, 100, 50, and 25 mu g/mL) groups, the final concentration was diluted to 16 x106 per straw. Semen samples were equilibrated at 4 degrees C for approximately 4 hours, then placed in French straws and frozen. After thawing, sperm motility and kinetic parameters, DNA integrity by single-cell gel electrophoresis, sperm abnormalities by liquid fixation. and lipid peroxidation levels by the colorimetric method was analyzed by Computer-Assisted Semen Analyzer. P added to the diluent showed no effect on motility and kinetic parameters at P25 and P50 (p>0.05), while P100 and P200 had a negative effect (p<0.001). The addition of P (25 and 50) showed a treatment effect on tail abnormality compared to C (p<0.05). Especially P50 had a positive effect on tail length, tail DNA, and tail movement, while P100 and P200 caused DNA damage (p<0.001). MDA levels increased in all P dose groups compared to C (p<0.001). This study has clearly demonstrated that P25 and P50 supplements could be used therapeutically to treat sperm tail abnormalities and prevent DNA damage in post-thawed bull sperm.
dc.identifier.doi10.24425/pjvs.2022.140834
dc.identifier.endpage12en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue1en_US
dc.identifier.pmid35575768
dc.identifier.scopusqualityQ3
dc.identifier.startpage5en_US
dc.identifier.urihttps:/dx.doi.org/10.24425/pjvs.2022.140834
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9415
dc.identifier.volume25en_US
dc.identifier.wosWOS:000779784000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPolska Akademia Nauk
dc.relation.ispartofPolish Journal of Veterinary Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryopreservation
dc.subjectDNA Damage
dc.subjectOxidative Stress
dc.subjectPropolis
dc.titleThe cryoprotective potential of propolis supplemented in frozen-thawed bull semen; biochemical and physiological findings
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
yeni-d.-2022.pdf
Boyut:
268.95 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu