Production and characterization of a cold-active and n-hexane activated lipase from a newly isolated Serratia grimesii RB06-22

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dc.contributor.authorUğur, Aysel
dc.contributor.authorBoran, Rukiye
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:29:32Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:29:32Z
dc.date.issued2014
dc.departmentSağlık Hizmetleri Meslek Yüksekokulu
dc.description.abstractA lipase-producing bacterium isolated from raw milk was identified as Serratia grimesii based on 16S rRNA sequence analysis. The extracellular lipase was partially purified by ammonium sulfate precipitation and ultrafiltration. Maximal activity was observed at 10 degrees C, the optimum pH was 8.0 and the enzyme was stable at 5-30 degrees C for 1 h. The K-m and V-max values were 1.7 mM and 0.3 mM/min respectively. It was found that the lipase had the highest hydrolytic activity towards sunflower oil and soybean oil. CaCl2 had a stimulatory effect on lipase activity, while EDTA and iodoacetic acid slightly inhibited the lipase activity and the enzyme was strongly inhibited by PMSF. The enzyme was compatible with various non-ionic surfactants as well as sodium cholate and saponin. In addition, the enzyme was relatively stable towards oxidizing agents. This lipase exhibited maximum activity in 35% n-hexane retaining about 2191% activity for 1 h.
dc.identifier.doi10.3109/10242422.2014.934684
dc.identifier.endpage230en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage222en_US
dc.identifier.urihttps://doi.org/10.3109/10242422.2014.934684
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6179
dc.identifier.volume32en_US
dc.identifier.wosWOS:000340296700004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.ispartofBiocatalysis and Biotransformation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectSerratia Grimesii
dc.subjectLipase
dc.subjectN-hexane Active
dc.subjectCold Active
dc.subjectEnzyme Characterization
dc.titleProduction and characterization of a cold-active and n-hexane activated lipase from a newly isolated Serratia grimesii RB06-22
dc.typeArticle

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