Antitumor and apoptotic effects of new-generation platinum compounds on human leukemia cell lines HL-60 and K562

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dc.authorid0000-0002-0091-6428
dc.authorid0000-0002-6078-7648
dc.authorid0000-0003-0206-3841
dc.authorid0000-0002-0033-6378
dc.authorid0000-0003-3946-4075
dc.authorid0000-0002-7228-0684
dc.contributor.authorKaracaer, Neslihan Tekin
dc.contributor.authorKerimoğlu, Barış
dc.contributor.authorBaran, Talat
dc.contributor.authorTarhan, Mehtap
dc.contributor.authorMenteş, Ayfer
dc.contributor.authorÖztürk, Kamile
dc.date.accessioned2022-01-27T08:09:56Z
dc.date.available2022-01-27T08:09:56Z
dc.date.issued2022
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractThe goal of this investigation is to report the fabrication, characterization, cytotoxicity, and apoptotic assessment of new platinum based compounds on K562 and HL-60 human leukemia cells. Two new platinum (II) compounds, Pt-5a and Pt-6a, were prepared and characterized by fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance spectroscopy ((HNMR)-H-1), environmental scanning electron microscopy (ESEM) and energy dispersive spectrometer (EDS) techniques. The cytotoxic activities of the compounds were evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. Caspase-3, B-cell lymphoma 2 (Bcl-2), and B-cell lymphoma 2 associated X protein (Bax) gene expressions were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) to illuminate the mechanism of apoptosis. The results present that the applied compounds exhibited dose-dependent cytotoxic effects in-vitro. Pt-5a and Pt-6a compounds caused a rise in Bax in HL-60 cells while a reduction in Bcl-2 was recorded in all applied doses. In HL-60 cells, an increase in caspase-3 was detected at doses of 25 mu M and 50 mu M of Pt-5a and 30 mu M of Pt-6a. The treatment with 40 mu M of Pt-5a increased caspase-3 and Bax in K562 cells compared with control cells. Bcl-2 was found to be low in 20 mu M of Pt-5a treatment in K562 cells. Pt-6a caused a significant increase in caspase-3 at the dose of 30 mu M in the same cells. It is proposed that the newly synthesized platinum compounds may prove to be significant in the development of anticancer-effective drugs as they trigger apoptosis in a dose-dependent manner.
dc.identifier.doi10.1007/s11756-021-00930-7
dc.identifier.endpage260en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage249en_US
dc.identifier.urihttps:/dx.doi.org/10.1007/s11756-021-00930-7
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9164
dc.identifier.volume77en_US
dc.identifier.wosWOS:000723524300003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofBiologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectApoptosis
dc.subjectBax
dc.subjectBcl-2
dc.subjectCaspase-3
dc.subjectPlatinum Compounds
dc.titleAntitumor and apoptotic effects of new-generation platinum compounds on human leukemia cell lines HL-60 and K562
dc.typeArticle

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