Comparative typing of L. delbrueckii subsp bulgaricus strains using multilocus sequence typing and RAPD-PCR

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dc.contributor.authorCebeci, Aysun
dc.contributor.authorGürakan, Güzin Candan
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:29:43Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:29:43Z
dc.date.issued2011
dc.departmentSabire Yazıcı Fen-Edebiyat Fakültesi
dc.description.abstractComparative typing analysis of 25 Lactobacillus delbrueckii subsp. bulgaricus strains, isolated from traditional yoghurts in Turkey, was performed by RAPD-PCR (randomly amplified polymorphic DNA-PCR) and MLST (multilocus sequence typing). RAPD-PCR analyses were performed using two primers; M13 and 1254. Primer 1254 produced better results than primer M13. The bands produced by primer 1254 were brighter and easier to interpret, and a higher number of bands were produced. In addition, clusters produced by primer 1254 were grouped according to the source of isolation. MLST analysis was performed using three genes, beta-gal, pheS and rpoA, and isolates were successfully characterized at strain level. To our knowledge, MLST analyses were used for the first time for strain level discrimination in L. delbrueckii subsp. bulgaricus. It enabled a detailed understanding of L. delbrueckii subsp. bulgaricus strains by using allele and sequence types' analysis. Both MLST and RAPD allowed for the typing of clusters according to the isolation source, while RAPD provided an increased differentiation. However, by increasing the number of genes analyzed, the discriminatory power of MLST could be increased.
dc.description.sponsorshipMiddle East Technical University (ODTU-BAP); DPT-YUUP
dc.description.sponsorshipThe authors would like to thank the Scientific Research Projects Fund of Middle East Technical University (ODTU-BAP) and DPT-YUUP for the partial financial support provided. Authors would also like to thank Ms. Neslihan Altay for isolation and phenotypical characterizations of strains from Turkish traditional yoghurts (unpublished results from her PhD thesis).
dc.identifier.doi10.1007/s00217-011-1526-5
dc.identifier.endpage385en_US
dc.identifier.issn1438-2377
dc.identifier.issn1438-2385
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage377en_US
dc.identifier.urihttps://doi.org/10.1007/s00217-011-1526-5
dc.identifier.urihttps://hdl.handle.net/20.500.12451/6199
dc.identifier.volume233en_US
dc.identifier.wosWOS:000294061700002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofEuropean Food Research and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectL. Delbrueckii Subsp Bulgaricus
dc.subjectYoghurt
dc.subjectTyping
dc.subjectRAPD
dc.subjectMLST
dc.titleComparative typing of L. delbrueckii subsp bulgaricus strains using multilocus sequence typing and RAPD-PCR
dc.typeArticle

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