Characterization of tongue worm (Pentastomida) chitin supports ?- rather than ?-chitin
Yükleniyor...
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier GmbH
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The higher order relationships of the Pentastomida have historically been tenuous. In one early and influential study, analysis of extracted chitin implicated that pentastomids possess ?-chitin, a paradoxical finding given that the ? allomorph is found uniformly in the cuticles of all arthropods and is considered as an apomorphic character of the Ecdysozoa. This result was further used to support the idea that the enigmatic pentastomids might be a wholly independent clade. More recent studies, however, firmly place the pentastomids within the Ecdysozoa, suggesting that the state of its chitin should be re-examined. In this study, we extracted high-quality chitin from the pentastomid, Leiperia cincinnalis, and carried out diagnostic analyses. Fourier Transform Infrared Spectroscopy (FT-IR) and ThermoGravimetric Analysis (TGA) demonstrated the conspicuous features of ?-chitin but not ?-chitin. Furthermore, transmission electron microscopy (TEM) of the cuticle of the anterior part of a specimen revealed nanofibres organized largely in a parallel configuration and scanning electron microscopy (SEM) of isolated cuticular chitin exhibited well-defined chitin bundles, these observations being more consistent with the known characteristics of ?-chitin. Collectively, our analyses demonstrate the clear existence of ?-chitin in a pentastomid and clarify a contentious issue using modern methods of analysis.
Açıklama
Kaya, Murat ( Aksaray, Yazar ) Akyüz, Lalehan ( Aksaray, Yazar )
Anahtar Kelimeler
Cuticle, Ecdysozoa, FT-IR, TGA, ?-chitin
Kaynak
Zoologischer Anzeiger
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
279
Sayı
-
