Preparation of Fe(III)-chelated poly (HEMA-MAH) cryogel for lysozyme adsorption
| dc.authorid | 0000-0002-2589-9919 | |
| dc.contributor.author | Ergün, Bahar | |
| dc.contributor.author | Derazshamshır, Ali | |
| dc.contributor.author | Odabaşı, Mehmet | |
| dc.date.accessioned | 2019-05-27T10:58:49Z | |
| dc.date.available | 2019-05-27T10:58:49Z | |
| dc.date.issued | 2007 | |
| dc.department | Sabire Yazıcı Fen Edebiyat Fakültesi | |
| dc.description | Odabaşı, Mehmet ( Aksaray, Yazar ) | |
| dc.description.abstract | Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [poly(HEMA-MAH)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-50 ?m size. Then the monolithic cryogel was loaded with Fe(III) ions to form the metal chelate with poly(HEMAMAH) cryogel. Poly(HEMA-MAH) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-MAH) monolithic cryogel was 2.82 g H2O/g cryogel. Poly(HEMA-MAH) cryogel containing 36.4 ?mol MAH/g was used in the adsorption/desorption of lysozyme from aqueous solutions. The nonspecific adsorption of lysozyme was very low (1.1 mg/g). The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the poly(HEMA-MAH) cyogel without significant loss of adsorption capacity | |
| dc.identifier.endpage | 145 | en_US |
| dc.identifier.issn | 1303-5002 | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.startpage | 143 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/1023 | |
| dc.identifier.volume | 35 | en_US |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | |
| dc.relation.ispartof | Hacettepe Journal of Biology and Chemistry | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Cryogel | |
| dc.subject | Lysozyme | |
| dc.subject | Immobilized Metal Affinity Chromatography | |
| dc.subject | Histidine | |
| dc.subject | Poly(HEMA) | |
| dc.subject | Kriyojel | |
| dc.subject | Lizozim | |
| dc.subject | İmmobilize Metal Afinite Kromatografisi | |
| dc.subject | Histidin | |
| dc.subject | Poli (HEMA) | |
| dc.title | Preparation of Fe(III)-chelated poly (HEMA-MAH) cryogel for lysozyme adsorption | |
| dc.type | Article |
