Preparation of Fe(III)-chelated poly (HEMA-MAH) cryogel for lysozyme adsorption
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Dosyalar
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [poly(HEMA-MAH)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-50 ?m size. Then the monolithic cryogel was loaded with Fe(III) ions to form the metal chelate with poly(HEMAMAH) cryogel. Poly(HEMA-MAH) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-MAH) monolithic cryogel was 2.82 g H2O/g cryogel. Poly(HEMA-MAH) cryogel containing 36.4 ?mol MAH/g was used in the adsorption/desorption of lysozyme from aqueous solutions. The nonspecific adsorption of lysozyme was very low (1.1 mg/g). The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the poly(HEMA-MAH) cyogel without significant loss of adsorption capacity
Açıklama
Odabaşı, Mehmet ( Aksaray, Yazar )
Anahtar Kelimeler
Cryogel, Lysozyme, Immobilized Metal Affinity Chromatography, Histidine, Poly(HEMA), Kriyojel, Lizozim, İmmobilize Metal Afinite Kromatografisi, Histidin, Poli (HEMA)
Kaynak
Hacettepe Journal of Biology and Chemistry
WoS Q Değeri
Scopus Q Değeri
Cilt
35
Sayı
2
