Evaluation of chromosomal DNA damage, cytotoxicity, cytostasis, oxidative DNA damage and their relationship with endocrine hormones in patients with acute organophosphate poisoning

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dc.contributor.authorGündoğan, Kürşat
dc.contributor.authorDönmez Altuntaş, Hamiyet
dc.contributor.authorHamurcu, Zuhal
dc.contributor.authorAkbudak, Ismail Hakkı
dc.contributor.authorSungur, Murat
dc.contributor.authorBitgen, Nazmiye
dc.contributor.authorBaşkol, Gülden
dc.contributor.authorBayram, Fahri
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:14:52Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:14:52Z
dc.date.issued2018
dc.departmentAksaray Teknik Bilimler Meslek Yüklsekokulu
dc.description.abstractPesticides are commonly used compounds in agriculture. Especially, organophosphates (OPs) are among the extensively used pesticides. Therefore, OPs poisoning is common, especially in underdeveloped and developing countries. Primary aim of this study was to research the effects of acute OPs poisoning on genome instability in the individuals' lymphocytes with acute OPs poisoning both by using the cytokinesis-block micronucleus cytome (CBMN-cyt) assay to examine chromosome/genome damage, cell proliferation index and cell death rate and by using the plasma 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels to determine oxidative DNA damage. Secondary aim of this study was also to assess whether a relation exists between endocrine hormones and the genome damage in acute OPs poisoning. In the study, blood samples were analysed of 13 patients before and after treatment admitted to the Department of Intensive Care Unit with acute OPs poisoning and of 13 healthy subjects of similar age and sex. The present study demonstrates that genome damage (micronucleus; MN and nucleoplasmic bridges; NPBs frequencies), apoptotic and necrotic cell frequencies increased in lymphocytes of patients with acute OPs poisoning before treatment and decreased after treatment. The present study also show that CBMN cyt assay parameters and 8-OHdG levels could be affected by some endocrine hormones such as E2, fT3, fT4, GH, IGF-1, FSH, LH, TSH, PRL, but not be related to ACTH and tT levels in acute OPs poisoning. In conclusion, it is believed that this is the first study to evaluate the chromosomal/oxidative DNA damage, cell proliferation, cell death and their associations with endocrine hormones in acute OPs poisoning. These preliminary findings need to be supported by further studies with larger sample sizes.
dc.description.sponsorshipResearch Fund of the Erciyes University [TSA-12-3792]
dc.description.sponsorshipThis work was supported by Research Fund of the Erciyes University (Project number: TSA-12-3792).
dc.identifier.doi10.1016/j.mrgentox.2017.11.005
dc.identifier.endpage7en_US
dc.identifier.issn1383-5718
dc.identifier.issn1879-3592
dc.identifier.pmid29307370
dc.identifier.scopusqualityQ3
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.mrgentox.2017.11.005
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4189
dc.identifier.volume825en_US
dc.identifier.wosWOS:000423001300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofMutation Research-Genetic Toxicology And Environmental Mutagenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEndocrine Hormones
dc.subjectDNA Damage
dc.subjectMN Frequency
dc.subject8-OHdG Levels
dc.subjectAcute Organophosphate Poisoning
dc.titleEvaluation of chromosomal DNA damage, cytotoxicity, cytostasis, oxidative DNA damage and their relationship with endocrine hormones in patients with acute organophosphate poisoning
dc.typeArticle

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