Immobilization of catalase via adsorption onto metal-chelated affinity cryogels

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dc.authoridTüzmen, Nalan -- 0000-0001-9863-7023;
dc.contributor.authorTüzmen, Nalan
dc.contributor.authorKalburcu, Tülden
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:20Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:20Z
dc.date.issued2012
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractA poly (acrylamide-allylglycidyl ether) [p(AAm-AGE)] cryogel was prepared by radical polymerization of acrylamide (AAm) and allylglycidyl ether (AGE). Cibacron Blue F3GA (CB) was covalently attached to the p(AAm-AGE) cryogel via the reaction between the chloride groups of the reactive dyes and the epoxide groups of the AGE. The CB-attached p(AAm-AGE) cryogel was chelated with Fe3+ ions. This immobilized metal ion affinity chromatography (IMAC) cryogel carrying 25.8 +/- 2.0 mu mol Fe3+ ions was used in adsorption studies to interrogate the effects of pH, protein initial concentration, flow rate, temperature and ionic strength on enzyme activity. Maximum adsorption capacities were found to be 75.7 +/- 1.2 mg/g for p(AAm-AGE)-CB-Fe3+ cryogels and 60.6 +/- 1.0 mg/g for p(AAm-AGE)-CB cryogels, respectively. The adsorbed amounts of catalase per unit mass of cryogel reached a plateau value at about 1.5 mg/mL at pH 6.0. The K-m values were found to be 0.73 +/- 0.02 g/L for the free catalase and 0.18 +/- 0.02 g/L for the immobilized catalase. The V-max value of free catalase (2.0 x 10(3) U/mg enzyme) was found to be lower than that of the immobilized catalase (2.5 x 10(3) U/mg enzyme). It was also observed that the enzyme could be repeatedly adsorbed and desorbed onto the p(AAm-AGE)-CB-Fe3+ cryogel. (C) 2011 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.procbio.2011.09.021
dc.identifier.endpage33en_US
dc.identifier.issn1359-5113
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2011.09.021
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5548
dc.identifier.volume47en_US
dc.identifier.wosWOS:000300133000004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCatalase
dc.subjectCryogel
dc.subjectIMAC
dc.subjectEnzyme Immobilization
dc.subjectAdsorption
dc.subjectDye Ligand
dc.titleImmobilization of catalase via adsorption onto metal-chelated affinity cryogels
dc.typeArticle

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