Development of a simple and rapid DNA extraction method for aspergillus Flavus-A

dc.contributor.authorSanio?lu Gölen, Gökçenur
dc.contributor.authorAkar, Kadir
dc.date.accessioned2024-09-30T07:22:51Z
dc.date.available2024-09-30T07:22:51Z
dc.date.issued2024
dc.departmentVeteriner Fakültesi
dc.description.abstractAspergillus species are known to be very important in human and domestic animal health. Aspergillus species commonly cause severe systemic and skin infections, as well as allergic lung diseases. With the development of PCR techniques, these methods are used to identify and diagnose fungi. DNA extraction from Aspergillus species is difficult because the fungal cell wall structure is very durable and complex. Fungal DNA extraction methods containing proteinase K and liquid nitrogen are widely used to break down the cell wall. However, these methods cause DNA loss during the extraction in Aspergillus species. In this study, on the contrary, the commonly used DNA extraction by means of ammonium hydroxide, which is generally used to break down chitin in DNA extraction of ticks and plants, is used. The efficiency of the cell wall lysis method from A. flavus with ammonium hydroxide was compared with methods containing proteinase K and liquid nitrogen. For this purpose, DNA extraction of A. flavus was tried using three different methods. As a result, the cell wall of A. flavus was lysed using ammonium hydroxide in this study. The obtained DNA's quality, concentration, and PCR performance were sufficient. This method has been evaluated as a faster, more straightforward, and more economical alternative.
dc.identifier.doi10.2478/acve-2024-0027
dc.identifier.endpage408en_US
dc.identifier.issn0567-8315
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage398en_US
dc.identifier.urihttps:/dx.doi.org/10.2478/acve-2024-0027
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12498
dc.identifier.volume74en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSciendo
dc.relation.ispartofActa Veterinaria
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAmmonium Hydroxide
dc.subjectAspergillosis
dc.subjectExtraction
dc.subjectLiquid Nitrogen
dc.subjectProteinase K
dc.titleDevelopment of a simple and rapid DNA extraction method for aspergillus Flavus-A
dc.typeArticle

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