USF1 Suppresses Expression of Fibrillar Type I, II, and III Collagen and pNP Adamts-3 in Osteosarcoma Cells
| dc.contributor.author | Alper, Meltem | |
| dc.contributor.author | Aydemir, T. | |
| dc.contributor.author | Köçkar, F. | |
| dc.date.accessioned | 2021-07-05T07:35:52Z | |
| dc.date.available | 2021-07-05T07:35:52Z | |
| dc.date.issued | 2021 | |
| dc.department | Teknik Bilimler Meslek Yüksekokulu | |
| dc.description | *Alper, Meltem ( Aksaray, Yazar ) | |
| dc.description.abstract | Abstract: Collagens are the main components of human tissues. Various regulatory factors and cytokines may influence expression levels for collagen-encoding genes, and, therefore, contrubite to some collagen-associated pathologies. In this study, we demonstrate regulatory effects of USF1 on expression of genes encoding fibrillar collagen types I, II, and III in osteoblastic Saos-2 and MG-63 cells. An ectopic expression of the human USF1 led to a decrease in both mRNA and protein expression levels of the collagen-encoding genes mentioned above. ADAMTS-3 is a proteinase primarily responsible for the amino-terminal cleavage of type I and type II collagen precursors. The ADAMTS-3 promoter region contains potential binding sites for USF1. Here we show that an overexpression of USF1 lead to a decrease in ADAMTS-3 mRNA and protein expression levels. In co-transfection studies, USF1 negatively regulated ADAMTS-3 promoter activity. Further, in EMSA studies, we showed that USF1 binds to the ADAMTS-3 promoter region. In conclusion, it seems that ADAMTS-3 and USF1 contribute to the regulation of collagen encoding genes in osteosarcoma. | |
| dc.identifier.doi | 10.1134/S0026893321030031 | |
| dc.identifier.endpage | - | en_US |
| dc.identifier.issn | 0026-8933 | |
| dc.identifier.issue | - | en_US |
| dc.identifier.pmid | 34432781 | |
| dc.identifier.scopusquality | Q4 | |
| dc.identifier.startpage | - | en_US |
| dc.identifier.uri | https:/dx.doi.org/10.1134/S0026893321030031 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12451/8309 | |
| dc.identifier.volume | - | en_US |
| dc.identifier.wos | WOS:000656110200003 | |
| dc.identifier.wosquality | Q4 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Pleiades Journals | |
| dc.relation.ispartof | Molecular Biology | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | ADAMTS-3 | |
| dc.subject | Collagen | |
| dc.subject | Osteosarcoma | |
| dc.subject | Transcriptional Regulation | |
| dc.subject | USF1 | |
| dc.title | USF1 Suppresses Expression of Fibrillar Type I, II, and III Collagen and pNP Adamts-3 in Osteosarcoma Cells | |
| dc.type | Article |
