Burkholderia multivorans SB6 lipase as a detergent ingredient: characterization and stabilization

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dc.contributor.authorBoran, Rukiye
dc.contributor.authorUğur, Aysel
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:14:57Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:14:57Z
dc.date.issued2016
dc.departmentSağlık Hizmetleri Meslek Yükseokulu
dc.description.abstractIn this study, 265 bacterial isolates were collected from kitchen wastewater samples using Rhodamine B agar medium. Of these, 115 isolates were found to respond positively to the addition of commercial detergents. Using 16S rRNA sequence analysis, the isolate demonstrating the high stability towards commercial detergents was identified as Burkholderia multivorans. An SB6 lipase with a molecular mass of 70 kDa was purified from B. multivorans. The purified enzyme showed optimal activity at pH 9.0 and 40 A degrees C and remained stable in the presence of various metal ions, surfactants, and oxidizing agents. The addition of boron compounds improved the pH stability and thermostability of the enzyme, which displayed stability against some commercial detergents; moreover, this stability increased when boron compounds were added to the incubation medium as stabilizers. These properties make SB6 lipase an ideal choice as an additive in detergent formulations.
dc.description.sponsorshipTUBITAK, The Scientific and Technical Research Council of Turkey [TBAG-210T084]
dc.description.sponsorshipThis study is a part of the Ph.D. work of Rukiye Boran. A part of this work was supported by TUBITAK, The Scientific and Technical Research Council of Turkey, through the grant number TBAG-210T084. We thank Dr. E. Sonay Elgin for help during the enzyme purification study. We would also like to express our gratitude to Dr. Omer Simsek for their kind help during the identification of bacterial strain.
dc.identifier.doi10.1007/s11743-015-1767-6
dc.identifier.endpage48en_US
dc.identifier.issn1097-3958
dc.identifier.issn1558-9293
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage39en_US
dc.identifier.urihttps://doi.org/10.1007/s11743-015-1767-6
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4227
dc.identifier.volume19en_US
dc.identifier.wosWOS:000367541300005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSPRINGER HEIDELBERG
dc.relation.ispartofJOURNAL OF SURFACTANTS AND DETERGENTS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLipase
dc.subjectDetergent
dc.subjectBoron
dc.subjectCharacterization
dc.subjectStabilization
dc.titleBurkholderia multivorans SB6 lipase as a detergent ingredient: characterization and stabilization
dc.typeArticle

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