First protein affinity application of Cu2+-bound pure inorganic nanoflowers

dc.authorid0000-0002-3288-132X
dc.contributor.authorÖnal, Burcu
dc.contributor.authorAcet, Ömür
dc.contributor.authorDzmitruk, Volha
dc.contributor.authorHalets-Bui, Inessa
dc.contributor.authorShcharbin, Dzmitry
dc.contributor.authorÖzdemir, Nalan
dc.contributor.authorOdabaşı, Mehmet
dc.date.accessioned2021-05-04T09:20:27Z
dc.date.available2021-05-04T09:20:27Z
dc.date.issued2022
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description*Önal, Burcu ( Aksaray, Yazar )
dc.description.abstractToday, a new kind of materials is introduced to separation media day by day to increase the efficiency of the separation processes, and multiple-petalled nanostructured materials are one of them. In this study, new pure inorganic copper phosphate nanoflowers (pCP-NFs) were synthesized, and some environmental conditions affecting on binding mechanism with human serum albumin were evaluated via changing medium pH, temperature, initial human serum albumin (HSA) amount and salt concentrations. Before experimental studies, pCP-NFs were subjected to some characterization tests such as scanning electron microscopy, energy-dispersive X-ray, X-ray diffraction and Fourier transform infrared spectroscopy. Besides a lot of valuable instrumental data, some obtained experimental ones as follows: after Cu2+ ions attachment to pCP-NFs as ligand, maximum HSA adsorption capacity of obtained Cu2+-pCP-NFs was found as 225.7 mg/g with an initial concentration of 1.5 mg/mL at pH 7 and 25 degrees C. Langmuir and Freundlich adsorption equations were evaluated for determination of appropriate adsorption model in interaction, and Langmuir model found as the fittest one with a R-2 of 0.9949 was also reviewed to determine Gibbs free energy between HSA and Cu2+-pCP-NFs interaction.
dc.identifier.doi10.1007/s00289-021-03557-5
dc.identifier.endpage-en_US
dc.identifier.issue-en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/ 10.1007/s00289-021-03557-5
dc.identifier.urihttps://hdl.handle.net/20.500.12451/7938
dc.identifier.volume-en_US
dc.identifier.wosWOS:000631742700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectPure Copper Phosphate Nanoflowers
dc.subjectImmobilized Metal Affinity Chromatography
dc.subjectProtein Separation
dc.subjectInorganic Sorbents
dc.titleFirst protein affinity application of Cu2+-bound pure inorganic nanoflowers
dc.typeArticle

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