Effect of additives on the post-thaw quality of Aksaray Malaklı dog semen

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dc.contributor.authorÖztürk, Caner
dc.contributor.authorGüngör, Ş.
dc.date.accessioned2022-06-20T07:36:08Z
dc.date.available2022-06-20T07:36:08Z
dc.date.issued2022
dc.departmentVeteriner Fakültesi
dc.description.abstractThe goal of this study was to determine effects of trehalose (T) and Trolox (TX) additives on post-thaw viability of canine sperm after freezing. The sperm-rich portions of ejaculates obtained from 15 dogs were divided into three aliquots, which were diluted with Tris-based extender (TBE) (control), TBE + T (25 mM), and TBE + TX (1 mM) and put into straws. The samples were equilibrated at 4 °C for 1.5 hours, then frozen in liquid nitrogen vapour for 15 minutes and plunged into liquid nitrogen. Plasma membrane and acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were evaluated after thawing the straws in a water bath at 37 °C for 30 seconds. Motility, percentage of live spermatozoa, and hypo-osmotic swelling (HOS) were determined using phase-contrast microscopy. Motility (53.00 ± 6.46%), percentage of live spermatozoa (62.97% ± 6.84%), and HOS (40.40 ± 7.11%) of T were significantly higher (P <0.05) than the control (40.50 ± 4.76%, 50.63% ± 4.78%, and 30.47% ± 5.59%) and TX (40.50 ± 4.76%, 50.96 ± 5.84%, and 33.02% ± 8.77%) respectively. The PMAI and HMMP levels were higher (P <0.05) in T (35.51±8.72 and 24.36±7.15%) than in the control (19.18 ± 3.49 and 12.04 ± 3.83%). The TX group (35.62 ± 8.21%) had significantly higher PMAI (P <0.05) than the control (12.04 ± 3.83%). Thus, addition of T protected canine spermatozoa against cryogenic injury. Nonetheless, additional dose-response trials are recommended to determine the effects of Trolox on the quality of canine semen more accurately.
dc.identifier.doi10.4314/sajas.v52i2.5
dc.identifier.endpage167en_US
dc.identifier.issn0375-1589
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage162en_US
dc.identifier.urihttps:/dx.doi.org/10.4314/sajas.v52i2.5
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9441
dc.identifier.volume52en_US
dc.identifier.wosWOS:000892246900005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSouth African Journal of Animal Sciences
dc.relation.ispartofSouth African Journal of Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcrosome Integrity
dc.subjectCryoprotection
dc.subjectMitochondrial Membrane Potential
dc.subjectPlasma Membrane
dc.subjectTrehalose
dc.subjectTrolox
dc.titleEffect of additives on the post-thaw quality of Aksaray Malaklı dog semen
dc.typeArticle

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