Whole cell imprinting based Escherichia coli sensors: A study for SPR and QCM

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dc.contributor.authorYılmaz, Erkut
dc.contributor.authorMajidi, Daryoush
dc.contributor.authorÖzgür, Erdo?an
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:19Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:19Z
dc.date.issued2015
dc.departmentFen-Edebiyat Fakültesi
dc.description.abstractThe detection of pathogenic bacteria is essential for a sustainable prevention of water quality and by this way prevention of life threatening illnesses. It is important to use rapid methods for pathogenic bacteria detection but traditional detection methods may take up to a whole week. In this report, we describe a molecular imprinting based bacteria sensor via whole cell imprinting for rapid detection of bacteria from water sources. Here, Escherichia coli (E. coli) is selected as model bacteria because of it is one of the most abundant pathogenic bacteria in ground water sources and may cause severe illnesses. The presence of E. coli in the water sources is an indicator of urban and agricultural runoffs, so monitoring E. coli contamination is important to preserve quality of the water sources. Traditional detection methods for E. coli include genomic analysis, antibody based assays, culture methods, fluorescence and microscopy. Disadvantages of traditional bacteria detection methods induce many researches on other detection methods like biosensors. In this study, a new label-free rapid and selective detection method was developed via micro contact imprinting of whole cell on both optical and mass sensitive devices. The amino acid based recognition element, N-methacryloyl-L-histidine methylester (a polymerizable form of histidine) was used in this study to obtain similar recognition as in natural antibodies.
dc.description.sponsorshipMinistry of Science, Industry and Technology of Republic of Turkey [1236.TGSD.2013]; Scientific and Technological Research Council (TUBITAK) of Republic of Turkey [2216]
dc.description.sponsorshipThis research has been carried out with supports from the Ministry of Science, Industry and Technology of Republic of Turkey with grant number 1236.TGSD.2013 and 2216 program of Scientific and Technological Research Council (TUBITAK) of Republic of Turkey.
dc.identifier.doi10.1016/j.snb.2014.12.032
dc.identifier.endpage721en_US
dc.identifier.issn0925-4005
dc.identifier.scopusqualityQ1
dc.identifier.startpage714en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2014.12.032
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5535
dc.identifier.volume209en_US
dc.identifier.wosWOS:000349082200095
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B: Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCell Imprinting
dc.subjectBacteria
dc.subjectPathogen Detection
dc.subjectSPR
dc.subjectQCM
dc.subjectBiosensor
dc.titleWhole cell imprinting based Escherichia coli sensors: A study for SPR and QCM
dc.typeArticle

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