Investigation of protective effects of rutin\cyclodextrin inclusion complex against testicular damage caused by diisononyl phthalate in rats

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dc.contributor.authorBozalı, Ramazan
dc.contributor.authorAkarsu, Serkan Ali
dc.contributor.authorGür, Cihan
dc.contributor.authorKüçükler, Sefa
dc.contributor.authorAkaras, Nurhan
dc.contributor.authorLeritürk, Mustafa
dc.contributor.authorSunar, Serhat
dc.contributor.authorKandemir, Fatih Mehmet
dc.date.accessioned2025-09-24T06:02:29Z
dc.date.available2025-09-24T06:02:29Z
dc.date.issued2025
dc.departmentTıp Fakültesi
dc.description.abstractThe aim of this study was to investigate the protective effects of Rutin\cyclodextrin (RUT\CD) complex in rats exposed to diisononyl phthalate (DINP). Materials and Methods: In the study, 35 male Sprague Dawley rats were used. The rats were randomly divided into five groups: Control, DINP, RUT\CD, DINP+RUT\CD100, and DINP+RUT\CD200. The control group received Tween 80 by oral gavage, while the DINP groups received DINP at a dose of 200 mg/kg/bw. RUT+CD groups received the RUT\CD complex by oral gavage. After 14 days of administration, rats were sacrificed, and testicular tissues were used for histopathological and biochemical analyses, and epididymal tissues were used for semen analysis. Results: DINP administration caused an increase in MDA level and a decrease in SOD, CAT, GPx1 enzyme activities, and GSH level in rats. RUT\CD administration decreased oxidative stress and increased antioxidant activity. In addition, DINP administration caused a decrease in Nrf-2 and HO-1 levels. DINP caused a significant increase in eIF2 alpha, ATF4, NF-kappa B, TNF-alpha, IL-1 beta, IL-6, Inos, and Cox2 levels in the testicular tissue of rats. RUT\CD administration decreased these levels in a dose-dependent manner. Apoptosis markers p53, Apaf-1, Bax, Bcl-2, and Caspase-3 mRNA transcript levels and Bax and Bcl-2 protein levels were significantly increased in the DINP-administered group. In the DINP+ RUT/CD group, these levels decreased in a dose-dependent manner. Moreover, DINP administration caused an increase in sperm DNA damage. Conclusion: DINP administration induced testicular toxicity by increasing oxidative stress, apoptosis, and inflammation, and changes in testicular histology. Moreover, RUT\CD administration attenuated DINP-induced toxic effects.
dc.identifier.doi10.22038/ijbms.2025.87549.18910
dc.identifier.endpage1391
dc.identifier.issn2008-3866 / 2008-3874
dc.identifier.issue10
dc.identifier.pmid40896694
dc.identifier.startpage1381
dc.identifier.urihttps://doi.org/10.22038/ijbms.2025.87549.18910
dc.identifier.urihttps://hdl.handle.net/20.500.12451/14535
dc.identifier.volume28
dc.identifier.wosWOS:001548122500010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.institutionauthorAkaras, Nurhan
dc.institutionauthorKandemir, Fatih Mehmet
dc.language.isoen
dc.publisherMashhad University Medical Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDINP
dc.subjectOxidative Stress
dc.subjectRutin\cyclodextrin
dc.subjectSperm Quality
dc.subjectTesticular Damage
dc.titleInvestigation of protective effects of rutin\cyclodextrin inclusion complex against testicular damage caused by diisononyl phthalate in rats
dc.typeArticle

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