Preparation and characterization of metal-chelated poly(HEMA-MAH) monolithic cryogels and their use for DNA adsorption

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dc.contributor.authorOdabaşı, Mehmet
dc.contributor.authorBaydemir, Gözde
dc.contributor.authorKarataş, Melike
dc.contributor.authorDerazshamshir, Ali
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:26:55Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:26:55Z
dc.date.issued2010
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractDNA adsorption properties of Zn(2+)-chelated supermacroporous poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [poly(HEMA-MAH)] monolithic cryogel column were investigated for the application of DNA-affinity adsorbents. The monolithic cryogel was loaded with Zn(2+) ions to form the metal-chelated affinity sorbent. Poly(HEMA-MAH) cryogel was characterized by swelling tests, FTIR, scanning electron microscopy (SEM), and elemental analysis. SEM analysis indicates that the cryogel have a heteroporous structure with interconnected pores of 10-50 mu m size, which ascribed to the porogens effect of frozen water crystals. Poly(HEMA-MAH) cryogel containing 45.8 mu mol MAH was used in the adsorption/desorption of DNA from aqueous solutions. The maximum amount of DNA adsorption was 32.93 mg/g in Tris buffer at pH 7.0. It was observed that DNA could be repeatedly adsorbed and desorbed with the poly(HEMA-MAH) cryogel without significant loss of adsorption capacity. As a result, these higher amounts of DNA adsorbed poly (HEMA-MAH) cryogels are expected to be good candidate for achieving higher removal of anti-DNA antibodies from systemic lupus erythematosus (SLE) patients plasma. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 116: 1306-1312, 2010
dc.identifier.doi10.1002/app.31505
dc.identifier.endpage1312en_US
dc.identifier.issn0021-8995
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage1306en_US
dc.identifier.urihttps://doi.org/10.1002/app.31505
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5742
dc.identifier.volume116en_US
dc.identifier.wosWOS:000275342200010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAffinity Chromatography
dc.subjectDNA Adsorption
dc.subjectMetal Chelate
dc.subjectHistidine
dc.subjectCryogels
dc.titlePreparation and characterization of metal-chelated poly(HEMA-MAH) monolithic cryogels and their use for DNA adsorption
dc.typeArticle

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