IL-6 upregulates a disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) in human osteosarcoma cells mediated by JNK pathway

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dc.contributor.authorAlper, Meltem
dc.contributor.authorKöçkar, Feray Tura
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:27:28Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:27:28Z
dc.date.issued2014
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractADAMTS-2 and ADAMTS-3 (a disintegrin and metalloproteinase with thrombospondin type 1 motif 2) belong to the procollagen aminoproteinase subfamily of ADAMTS proteases. They play crucial roles in the collagen metabolism. To understand the regulation of ADAMTS-2 gene expression in osteoblastic cells, we have cloned a functional 760 bp of human ADAMTS-2 promoter. Sequence analysis of the ADAMTS-2 promoter region showed the absence of a TATA box, but identified a GC box, a CpG island, several GAGA boxes and several transcriptional factor binding sites, which may be valuable in the regulation of ADAMTS-2 transcription. We also elucidated that Interleukin 6 (IL-6) increases ADAMTS-2 and ADAMTS-3 mRNA and protein levels in different osteosarcoma cell lines namely, MG-63 and Saos-2. IL-6 also increases the transcriptional activation of the ADAMTS-2 gene promoter. Pathway inhibition studies revealed that ADAMTS-2 upregulation by IL-6 was mediated by JNK pathway.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [212T200]; Balikesir University Scientific Research Projects Unit (BAP) [2010/39]
dc.description.sponsorshipThis work was supported mainly by the Scientific and Technological Research Council of Turkey (TUBITAK) (212T200) and partially by the Balikesir University Scientific Research Projects Unit (BAP) (2010/39).
dc.identifier.doi10.1007/s11010-014-2056-9
dc.identifier.endpage175en_US
dc.identifier.issn0300-8177
dc.identifier.issn1573-4919
dc.identifier.issue01.Feben_US
dc.identifier.pmid24752352
dc.identifier.scopusqualityQ1
dc.identifier.startpage165en_US
dc.identifier.urihttps://doi.org/10.1007/s11010-014-2056-9
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5868
dc.identifier.volume393en_US
dc.identifier.wosWOS:000338348700017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular and Cellular Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectADAM
dc.subjectADAMTS
dc.subjectInterleukin
dc.subjectOsteoblasts
dc.subjectJun N terminal kinase (JNK)
dc.subjectExtracellular matrix proteins
dc.titleIL-6 upregulates a disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) in human osteosarcoma cells mediated by JNK pathway
dc.typeArticle

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