İnaktif bakterin aşılarda Bor'un adjuvan etkinliğinin karşılaştırmalı olarak değerlendirilmesi
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Tarih
2018
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TÜBİTAK
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çiftlik hayvanlarının sağlıklı yetiştirilmesinde en etkili, pratik ve ekonomik uygulama aşılamadır. Ülkemiz Veteriner aşı pazar payı yaklaşık olarak 250 milyon TL'dir. Ancak aşı ve aşı ham maddeleri yönü ile büyük oranda dışa bağımlıdır. Dünya rezervlerinin % 72'sine sahip olduğumuz Bor'un; bitki, insan ve hayvanlarda pek çok fonksiyon üzerinde olumlu etkileri belirlenmiştir. Ancak, Bor bileşiklerinin, aşılarda adjuvan etkinliklerinin belirlenmesine yönelik herhangi bir çalışmaya rastlanmamıştır. Bu amaçla bu projede fare modelinde, Veteriner Hekimlikte yaygın kullanılan adjuvanlar ile Bor'un potansiyel adjuvan etkisi karşılaştırılmıştır. Projede öncelikle aşı antijeni ve challenge suşu olarak kullanılacak Staphylococcus aureus izolatları belirlenmiştir. Bu amaçla, mastitisli sütlerden izole edile "80" S. aureus’un, virülens ve patojenite ile ilişkili fenotipik ve genotipik özellikleri belirlenmiştir. Seçilen aşı antijen ile, Borik asit (BA), Alüminyum hidroksit (Al(OH)3), Montanide-ISA 50 (ISA-50), Montanide-ISA 206 (ISA-206) ve ISA-50+BA, ISA-206+BA adjuvanlı aşılar hazırlanmıştır. Challenge suşunun, çalışmada kullanılan 6-8 haftalık Balb-C fareler için LD50 dozu belirlenmiştir. Oluşturulan “challenge” ve “seropotens” gruplarındaki fareler aşılanarak, challenga karşı koruma, challenge bakterisinin iç organlardan temizlenme oranı, iç organlardaki ve aşı uygulama yerindeki histopatolojik değişklikler, immun yanıt ve yangı reaksiyonları karşılaştırmalı olarak değerlendirilmiştir. Challenge suşunun LD50 dozu 2,5x108 bakteri olarak belirlenmiştir. Challenge sonrası nisbi hayatta kalma yüzdesi kontrol grubunda %50, ISA-206 grubunda %75, diğer gruplarda ise %100 olarak gözlenmiştir. BA’in, ISA-50 ve IS-206 adjuvanlara bağlı gelişen lokal yangısal reaksiyonları azalttığı belirlendi. BA aşı grubunda, challenge sonrası iç organlardaki bakteri yükü ALOH aşı grubu ile yakın olarak tespit edilidi. BA adjuvanlı aşı ile aşılanan farelerde kontrollerden daha yüksek (P<0.05) ve ALOH’e yakın düzeyde anti-S. aureus antikor oluştuğu belirlendi. Sonuç olarak BA’in inaktif aşılarda adjuvan potansiyelinin olduğu ancak hedef hayvan çalışmalarına ihtiyaç duyulduğu düşünüldü.
Vaccination is the most effective, practical and economic application for healthy livestock. Veterinary vaccine market share is approximately 250 million Turkish Lira in Turkey. But, It is largely dependent on outside supply for the vaccines and the vaccine raw materials. Turkey has 72% of the world Boron mine reserves. Positive effects of Boron were determined on plant, humans and animals. No study could be come across on adjuvant activity of Boron compounds in the literature. In this project possible adjuvant activity of Boron will be compared with commonly used adjuvants in veterinary medicine using a mice model. Firstly, S. aureus isolates used as vaccine antigens and challenge strains was selected in the project. For this purpose, phenotypic and genotypic features of 80 S. aureus, isolated from masitic milk samples of cattle, were detected. Vaccine candidates were prepared using Boric acid (BA), Aluminum Hydroxide (Al(OH)3), Montanide-IS 50 (IS-50), Montanide-ISA 206 (ISA-206), IS-50 + BA, ISA-206 + BA. LD50 of challenge strain was determined for 6-8 week old Balb-C mice used in the study. Mice in the challenge and seropotence groups were vaccinated and protection against challenge, clearance rate of challenge bacterium from internal organs, histopathological changes in internal organs and vaccination site, immunological response and inflammatory reactions were evaluated, comparatively. LD50 of challenge strain was detected as 2,5x108 bacteria. After challenge, RPS was 50%, 75% and 100% in the control, ISA-206 and other groups, respectively. BA has been reduce to local inflammatory reactions due to ISA-50 and IS-206 adjuvants. In the BA vaccine group, the bacterial load in the internal organs after challenge was closely related to the ALOH vaccine group. In mice vaccinated with BA adjuvanted vaccine, higher anti-S. aureus antibody concentration were detected than controls and near ALOH. In conclusion, it is thought that BA has an adjuvant potential in inactivated vaccines, but target animal studies are needed.
Vaccination is the most effective, practical and economic application for healthy livestock. Veterinary vaccine market share is approximately 250 million Turkish Lira in Turkey. But, It is largely dependent on outside supply for the vaccines and the vaccine raw materials. Turkey has 72% of the world Boron mine reserves. Positive effects of Boron were determined on plant, humans and animals. No study could be come across on adjuvant activity of Boron compounds in the literature. In this project possible adjuvant activity of Boron will be compared with commonly used adjuvants in veterinary medicine using a mice model. Firstly, S. aureus isolates used as vaccine antigens and challenge strains was selected in the project. For this purpose, phenotypic and genotypic features of 80 S. aureus, isolated from masitic milk samples of cattle, were detected. Vaccine candidates were prepared using Boric acid (BA), Aluminum Hydroxide (Al(OH)3), Montanide-IS 50 (IS-50), Montanide-ISA 206 (ISA-206), IS-50 + BA, ISA-206 + BA. LD50 of challenge strain was determined for 6-8 week old Balb-C mice used in the study. Mice in the challenge and seropotence groups were vaccinated and protection against challenge, clearance rate of challenge bacterium from internal organs, histopathological changes in internal organs and vaccination site, immunological response and inflammatory reactions were evaluated, comparatively. LD50 of challenge strain was detected as 2,5x108 bacteria. After challenge, RPS was 50%, 75% and 100% in the control, ISA-206 and other groups, respectively. BA has been reduce to local inflammatory reactions due to ISA-50 and IS-206 adjuvants. In the BA vaccine group, the bacterial load in the internal organs after challenge was closely related to the ALOH vaccine group. In mice vaccinated with BA adjuvanted vaccine, higher anti-S. aureus antibody concentration were detected than controls and near ALOH. In conclusion, it is thought that BA has an adjuvant potential in inactivated vaccines, but target animal studies are needed.
Açıklama
Aras, Zeki (Aksaray, Yazar) Bu çalışma TÜBİTAK tarafından 115O826 numaralı proje kapsamında desteklenmiştir.
Anahtar Kelimeler
Aşı, Bor, Adjuvan, Alüminyum Hidroksit, Montanide ISA-50, Montanide ISA-206, Fare Modeli, Vaccine, Boron, Adjuvant, Aluminum Hydroxide, Montanide ISA-50, Montanide ISA-206, Mice Model
Kaynak
TÜBİTAK TOVAG Proje; 2018
WoS Q Değeri
Scopus Q Değeri
Cilt
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