Cryogel disks for lactase immobilization and lactose-free milk production
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study aims to optimize lactase immobilization onto Fe-chelated cryogel disks. p(AAm-HEMA) cryogel disks were prepared by free radical polymerization and chelated with Fe ions to prepare IMAC material. The lactase immobilization was optimized after characterization by physical analysis. Lactase activity assays were performed with ONPG and lactose substrates. The maximum amount of immobilized lactase was determined as 107.2 ± 4.3 mg/g at 1.5 mg/mL and pH 4.5. The optimum pH of immobilized lactase was determined as pH 5.0, and the immobilized lactase showed notably higher catalytic activity at pH 3.0 compared to free lactase. The optimum temperature of lactase shifted from 50 °C to 60 °C, and the immobilized lactase presented higher activity at 60 °C and 65 °C than the free lactase. Higher thermal activities were achieved for all temperature values after immobilization. The catalytic effectiveness of lactase was not affected, and similar KM values were calculated for lactose. The immobilized lactase lost 29.2% of its initial activity at the end of 70 days, and preserved 64.9% of initial activity after 25-runs. All lactose in milk was hydrolysed by lactase immobilized cryogel disks in 5 h. The lactase immobilized cryogel disks present encouraging opportunities for lactose-free milk preparation in food industry.
Açıklama
Anahtar Kelimeler
Cryogel, Immobilization, Lactose-free Milk, Metal Chelate, ?-galactosidase
Kaynak
LWT
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
154
Sayı
-
