A novel chromatographic media: Histidine-containing composite cryogels for HIgG separation from human serum
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Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Histidine-containing microspheres (HCM) with 2 mu m in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.
Açıklama
Anahtar Kelimeler
HIgG Adsorption, Monolithic Composite Cryogel Column, Histidine Ligand, Microsphere Embedding
Kaynak
Affinity Chromatography: Methods and Protocols, 3rd Edition
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
1286
