Immobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater

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dc.contributor.authorAcet, Ömür
dc.contributor.authorİnanan, Tülden
dc.contributor.authorKoç, Eda Öndül
dc.contributor.authorSert, Buse
dc.contributor.authorÖnal Acet, Burcu
dc.contributor.authorOdabaşı, Mehmet
dc.contributor.authorOcakoğlu, Kasım
dc.contributor.authorDizge, Nadir
dc.date.accessioned2023-10-09T06:32:03Z
dc.date.available2023-10-09T06:32:03Z
dc.date.issued2023
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstract?-amylase is an important enzyme for textile, food, paper, and the pharmaceutical industrial areas. In this study, Ni2+ attached carbon felt structures with nitrogen active site (Ni2+-N-ACF) are produced. The surface morphologies of the N-ACF and Ni2+-N-ACF are investigated by means of scanning electron microscopy (SEM) analysis. Ni2+ ions binding on the N-ACFs are determined by energy dispersive X-ray (EDX) analysis and a graphite furnace atomic absorption spectrometer (AAS). The effect of pH, ionic strength, initial ?-amylase concentration, and temperature parameters is investigated for ?-amylase immobilization on Ni2+-N-ACF structures. In addition, pH and temperature effect on the activities of the free and the immobilized amylase, kinetic parameters, storage, and operational stabilities are made. Lastly, starch degradation in potato waste water is tested on Ni2+-N-ACF. The obtained results show that ?-amylase immobilized Ni2+-N-ACF can be used for starch degradation on an industrial scale.
dc.identifier.doi10.1002/star.202300033
dc.identifier.issn0038-9056
dc.identifier.scopusqualityQ2
dc.identifier.urihttps:/dx.doi.org10.1002/star.202300033
dc.identifier.urihttps://hdl.handle.net/20.500.12451/11095
dc.identifier.wosWOS:001115819400016
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley and Sons Inc
dc.relation.ispartofStarch/Staerke
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectActivated Carbon Felt
dc.subjectActivation
dc.subjectImmobilization
dc.subjectStarch Degradation
dc.titleImmobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater
dc.typeArticle

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