Effects of different doses of trehalose supplementation in egg yolk extender in frozen-thawed Angora buck semen

Basit Öğe Kaydı
dc.contributor.authorTuncer, Pürhan Barbaros
dc.contributor.authorTaşdemir, Umut
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorÖzgürtaş, Taner
dc.contributor.authorCoşkun, Erdem
dc.contributor.authorErol, Halil
dc.contributor.authorAydın, Fevzi Nuri
dc.contributor.authorGürcan, İsmail Safa
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T08:22:59Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T08:22:59Z
dc.date.issued2013
dc.departmentVeteriner Fakültesi
dc.description.abstractFew studies have been done on the effects of trehalose supplementation in the cryopreservation of Angora buck semen. The objective of present study was to investigate the effects of the addition of trehalose at different doses in semen extenders, on in vitro semen quality parameters, anti-oxidant enzymes activities and DNA damage after the freeze-thaw process in Angora buck semen. Semen samples from 5 mature Angora bucks (3 and 4 years of age) were used in this study. The bucks, belonging to the Livestock Central Research Institute were maintained under uniform breeding condition. A total number of 40 ejaculates were collected twice a week from the bucks using an artificial vagina, during the breeding season and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 7 equal aliquots and diluted (37. °C) with base extenders supplemented with the trehalose (12.5, 25, 50, 75, 100 and 150. mM), and a base extender with no additives (control). Diluted samples were aspirated into 0.25. ml French straws, and equilibrated at 5. °C for 4. h and then were frozen at a digital freezing machine. The freezing extender supplemented with 50. mM trehalose led to the greatest percentages of CASA motility (53.6. ± 4.69), in comparison to the other groups after the freeze-thawing process (P < 0.05). The addition of different doses of trehalose did not provide any significant effect on the percentages of post-thaw sperm motion characteristics (VAP, VSL and LIN), compared to the control (P > 0.05). The freezing extender with 150. mM trehalose group led to the highest percentages of acrosome abnormalities (P < 0.05) and 50. mM trehalose group had the lowest percentages of total abnormalities (P < 0.001), in comparison to the others. There were no significance differences in the DNA integrity among treatment groups (P > 0.05). The different doses of trehalose did not show any effectiveness on the maintenance GPx, LPO, GSH, CAT and total antioxidant activities, when compared to the control (P > 0.05). Therefore, the additions of 50. mM and 75. mM doses of trehalose will be useful in increasing post thaw motility on Angora buck semen. © 2013 Elsevier B.V.
dc.description.sponsorshipMinistry for Food, Agriculture, Forestry and Fisheries
dc.description.sponsorshipThis study was supported by the Republic of Turkey, Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policy (GDAR) Project number: 09/08/04/01.
dc.identifier.doi10.1016/j.smallrumres.2013.04.012
dc.identifier.endpage389en_US
dc.identifier.issn0921-4488
dc.identifier.issue02.Maren_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage383en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.smallrumres.2013.04.012
dc.identifier.urihttps://hdl.handle.net/20.500.12451/2684
dc.identifier.volume113en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofSmall Ruminant Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAngora Buck
dc.subjectCASA Parameters
dc.subjectCOMET Test
dc.subjectCryopreservation
dc.subjectOxidative Stress
dc.subjectTrehalose
dc.titleEffects of different doses of trehalose supplementation in egg yolk extender in frozen-thawed Angora buck semen
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
tuncer-purhan barbaros-2013.pdf.pdf
Boyut:
201.46 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu