A long noncoding RNA lincRNA EPS expression level in renal anemia in chronic kidney disease

Chronic Kidney Disease (CKD) is a long-term medical condition characterized by the gradual loss of kidney function over time. Anemia is one of the most important causes of morbidity in patients with CKD. Anemia negatively affects the quality of life and mortality of patients and increases the progression of kidney disease. This is the first study to indicate lincRNA_EPS expression levels in CKD patients with renal anemia. Material and Methods: A total of 59 people were included in this study with 40 patients and 19 control groups. Analyzes of lncRNA expression levels were performed by RT-qPCR method. Total RNA (including miRNA and lncRNA) was obtained from serum and plasma using an RNA isolation kit. Then, RNA concentration and purity were determined spectrophotometrically. The integrity of total RNA was determined by agarose gel electrophoresis. Results: This study concluded that the lincRNA-EPS expression values were found to be significantly higher in the CKD group. There was no statistically significant correlation between the eGFR level of the patients and the lincRNA-EPS expression levels. However, a statistically significant positive correlation was found between eGFR values and hemoglobin levels in the patient group. Discussion: Considering the increased lincRNA-EPS expression level in CKD patients with renal anemia and its inverse correlation with EPO usage, a potential mechanism for facilitating erythropoiesis may involve both elevated lincRNA-EPS expression and suppression of the pro-apoptotic gene Pycard. Larger and more clinical studies are needed to fully elucidate the mechanism of action of the renal anemia-EPOlincRNA-EPS relationship. In the light of these studies, it will be possible to identify new factors that play a role in the formation mechanisms of renal anemia and to evaluate new treatment alternatives.