Determination of the protective impact of hypericum perforatum extract against ovarian ischemia and reperfusion in rats

dc.authorid0000-0001-6422-9582
dc.authorid0000-0001-5631-2335
dc.authorid0000-0002-1883-6055
dc.authorid0000-0003-3745-8173
dc.authorid0000-0002-0659-3084
dc.authorid0000-0002-3584-5252
dc.authorid0000-0002-0715-2707
dc.authorid0000-0002-6293-3338
dc.authorid0000-0002-7279-5995
dc.contributor.authorBingöl, Seyit Ali
dc.contributor.authorYiğit, Serdar
dc.contributor.authorTaşkın, Ergin
dc.contributor.authorKaçı, Fatma Necmiye
dc.contributor.authorYayla, Muhammed
dc.contributor.authorKılıç Baygutalp, Nurcan
dc.contributor.authorToktay, Erdem
dc.contributor.authorEyerci, Nilnur
dc.contributor.authorÇılgın, Hasan
dc.date.accessioned2024-08-02T08:45:50Z
dc.date.available2024-08-02T08:45:50Z
dc.date.issued2024
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractOvarian torsion is an important gynaecological emergency because it causes ischemia in ovary. Our aim is to research the effects of Hypericum perforatum on p53, TNF-alpha, CAT, SOD, GSH, PTEN and is haPI3K/Akt/mTOR pathway in ovarian of rats with ischemia reperfusion (I/R) injury. Materials and Methods: The study included 56 adult female rats which were allocated to 7 groups; control, ischemia, ischemia 300HP, ischemia 600HP, I/R, I/R 300HP and I/R 600HP. H. perforatum was applied to ischemia 300HP, ischemia 600HP, I/R 300HP and I/R 600HP groups at the dose of 300 mg/kg or 600 mg/kg by oral gavage. Collected ovarian tissues were examined by light microscopy, biochemical and real -time PCR techniques. Results: In histological examination, the least degeneration was seen in 600HP group among other groups, apart from control group. TUNEL results showed that apoptosis level of other groups was lower than that of ischemia and I/R groups. CAT and GSH levels of I/R 600HP group increased. The highest level of PTEN and mTOR was found in the ischemia group. The Akt level was lower in I/R and I/R 300HP groups but its level was close to that of control and I/R 600HP groups. TNF-alpha and p53 mRNA expressions in I/R group were increased. Conclusions: The result of the study showed that H. perforatum (600 mg/kg) had an effect on tissue degeneration, CAT and GSH levels, and also TNF-alpha and p53 mRNA expressions levels. We suggest that H. perforatum can be considered as an agent to protect against tissues damage during ischemia reperfusion.
dc.identifier.doi10.34172/cjmb.2024.3720
dc.identifier.endpage68en_US
dc.identifier.issn2148-9696
dc.identifier.issue2en_US
dc.identifier.startpage62en_US
dc.identifier.urihttps:/dx.doi.org/10.34172/cjmb.2024.3720
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12309
dc.identifier.volume11en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherAras Part Medical international Press
dc.relation.ispartofCrescent Journal of Medical and Biological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOvary
dc.subjectIschemia/reperfusion
dc.subjectAntioxidant
dc.subjectp53
dc.subjectPTEN
dc.titleDetermination of the protective impact of hypericum perforatum extract against ovarian ischemia and reperfusion in rats
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Yükleniyor...
Küçük Resim
İsim:
bingol-seyit ali-2024.pdf
Boyut:
1.4 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama: