A novel affinity disks for bovine serum albumin purification

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dc.authoridTuzmen, Nalan -- 0000-0001-9863-7023; AKGOL, Sinan -- 0000-0003-2836-7181; AKTAS UYGUN, Deniz -- 0000-0002-5633-2704
dc.contributor.authorTüzmen, Nalan
dc.contributor.authorKalburcu, Tülden
dc.contributor.authorUygun, Deniz Aktaş
dc.contributor.authorAkgöl, Sinan
dc.contributor.authorDenizli, Adil
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-29T19:28:04Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-29T19:28:04Z
dc.date.issued2015
dc.departmentAksaray Teknik Bilimler Meslek Yüksekokulu
dc.description.abstractThe adsorption characteristics of bovine serum albumin (BSA) onto the supermacroporous poly(hydroxyethylmethacrylate)-Reactive Green 19 [p(HEMA)-RG] cryogel disks have been investigated in this paper. p(HEMA) cryogel disks were prepared by radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. Reactive Green (RG) 19 was covalently attached to the p(HEMA) cryogel disks. These disks were used in BSA adsorption studies to interrogate the effects of pH, initial protein concentration, ionic strength, and temperature. BSA adsorption capacity of the p(HEMA)-RG cryogel disk was significantly improved after the incorporation of RG. Adsorption capacity reached a plateau value at about 0.8 mg/mL at pH 4.0. The amount of adsorbed BSA decreased from 37.7 to 13.9 mg/g with increasing NaCl concentration. The enthalpy of BSA adsorption onto the p(HEMA)-RG cryogel disk was calculated as -58.4 kJ/mol. The adsorption equilibrium isotherm was fitted well by the Freundlich model. BSA was desorbed from cryogel disks (over 90 %) using 0.5 M NaSCN, and the purity of desorbed BSA was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The experimental results showed that the p(HEMA)-RG cryogel disks have potential for the quick protein separation and purification process.
dc.identifier.doi10.1007/s12010-014-1273-8
dc.identifier.endpage468en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue1en_US
dc.identifier.pmid25308615
dc.identifier.scopusqualityQ2
dc.identifier.startpage454en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-014-1273-8
dc.identifier.urihttps://hdl.handle.net/20.500.12451/5979
dc.identifier.volume175en_US
dc.identifier.wosWOS:000348102900036
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHumana Press
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine Serum Albumin
dc.subjectAffinity Adsorption
dc.subjectCryogels
dc.subjectDye Ligand
dc.subjectBlood
dc.titleA novel affinity disks for bovine serum albumin purification
dc.typeArticle

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