Lipopolisakkarit ile oluşturulan hipotermiye eşlik eden hormonal senkronizasyon
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Tarih
2011
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info:eu-repo/semantics/openAccess
Özet
Bu araştırmada, sıçanlara (Wistar albino) intraperitonal (250 µg/kg, ip) olarak lipopolisakkarit (LPS, E.coli serotip O111:B4) uygulanarak yapılan deneyler sonucunda hipotermi (?T: - 1.52±0.12 ºC) oluşturuldu. Hipoterminin değişik evrelerine [başlangıç (69.63±3.08 dak.), en derin (100.88±3.25 dak.) ve bitiş noktası (155.88±9.65 dak.)] denk gelen zaman dilimlerinde gruplar oluşturuldu. Hipotermi grupları ve kontrol (% 0,09 tuzlu su, 0.5 ml/kg, ip) grubu sıçanlardan intrakardiyak yolla kan örnekleri alındı. Kan örneklerinde, hipotalamo-hipofizer tiroit (HPT) ekseni hormonları [tiroit uyarıcı hormon (TSH), serbest triiyodotironin (ST3), serbest tiroksin (ST4)], hipotalamo-hipofizer adrenal korteks (HPA) ekseni hormonları [kortikotropin salıverici hormon (CRH), adrenokortikotropik hormon (ACTH), kortikosteron (KORT)], leptin (Lep) ve eritropoietin (EPO) hormonu düzeylerini belirlemek için analizler (ELISA) yapıldı. Belirlenen hormon düzeyleri, istatistiksel (ANOVA ile) olarak kontrol grubu ile hipotermi grupları karşılaştırıldı ve aralarındaki değişimin (artış/azalış) önemi belirlendi. Kontrol grubuna göre karşılaştırma sonucunda, TSH, CRH ve EPO hormon düzeylerindeki değişim önemsiz, ST4 (başlangıç noktası) hormon düzeyindeki artış, ST3 (en derin ve bitiş noktası), ACTH (başlangıç noktası), KORT (en derin noktası) ve Lep (bitiş noktası) hormonları düzeyindeki azalmada görülen değişimin anlamlı (P<0.05) olduğu tespit edildi. Hormonal değişimin anlamlı (P<0.05) olduğu hipotermi grupları, hipotermiyi ve tümör nekroz faktör-alfa (TNF-?) salınımını bloke ettiği bilinen SC-560 (1 mg/kg, sk) kullanılarak tedavi edildi. LPS ile oluşturulan hipotermi evrelerine denk gelen zaman dilimlerinde kan örnekleri alınarak hormonal analizleri yapıldı. Analiz sonuçları kontrol grubu ile istatistiksel olarak karşılaştırıldı. Tedavi sonucunda, LPS ile oluşturulan hipoterminin inhibe olduğu belirlenerek, hipoterminin en derin noktasında (?T: -0.57±0.04 ºC) ve hipoterminin bitiş anında (?T: 0.81±0.09 ºC) vücut sıcaklıkları ölçüldü. Aynı zamanda hormonal düzeyde görülen anlamlı (P<0.05) değişimin, ST4, ACTH, KORT ve Lep hormon düzeylerinde ortadan kalkarak normale döndüğü, ST3 hormon düzeyinde ise devam ettiği görüldü. Sonuçlar, LPS ile oluşturulan hipotermi ve hipotermiye eşlik eden hormonal senkronizasyon üzerinde, SC-560 ile tedavi dozunun kritik öneminin olabileceğini düşündürdü.
In this research, hypothermia (?T: -1.52±0.12 ºC) is induced as a result of the experiments involving intraperitonal (250 µg/kg, ip) injection of lipopolysaccharide (LPS, E.coli serotype O111:B4) on rats (Wistar albino). Groups have been formed for the time intervals corresponding to different phases [start (69.63±3.08 min.), deepest (100.88±3.25 min.) and end (155.88±9.65 min.) points] of the hypothermia. Blood samples have been collected by intracardiac method, from the rats of the hypothermia and the control groups (0.09% salin, 0.5 ml/kg, ip). Analyses (ELISA) have been performed on the blood samples to determine the levels of hypothalamic-pituitary-thyroid (HPT) axis hormones [thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4)], hypothalamic-pituitary-adrenal (HPA) axis hormones [corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT)], leptin (Lep) hormone and erythropoietin (EPO) hormone. The control and hypothermia groups have been statistically compared (via ANOVA) with regard to the determined hormone levels and the significance of the changes (increase/decrease) between groups have been determined. As a result of the comparison to the control group, TSH, CRH, and EPO, the hormone levels change insignificant, the changes in the increase of FT4 (start point) hormone level and the changes in the decrease of FT3 (the deepest and end points), ACTH (start point), CORT (the deepest point), and Lep (end point) hormone levels have been found to be statistically significant (P<0.05). The hypothermia groups for which the hormonal changes are significant (P<0.05), have been treated with SC560 (1 mg/kg, sk) which is known to block both the hypothermia and the stimulation of the tumor necrosis factor-alpha (TNF-?). Hormonal analyses have been performed on the blood samples collected in the time intervals corresponding to the phases of the hypothermia induced by LPS. The results of these analyses have been statistically compared to the control group. As a result of the treatment, having determined that the LPS-induced hypothermia has been inhibited, the body temperatures at the deepest (?T: -0.57±0.04 ºC) and end (?T: 0.81±0.09 ºC) points of the hypothermia have been measured. At the same time, the significant (P<0.05) changes found in the hormonal levels have been normalized and vanished for the FT4, ACTH, CORT, and Lep hormone levels, while these changes have sustained for the FT3 hormone level. The results have led to thoughts that the treatment dose of SC-560 may have a critical significance on the LPS-induced hypothermia and the accompanying hormonal synchronization.
In this research, hypothermia (?T: -1.52±0.12 ºC) is induced as a result of the experiments involving intraperitonal (250 µg/kg, ip) injection of lipopolysaccharide (LPS, E.coli serotype O111:B4) on rats (Wistar albino). Groups have been formed for the time intervals corresponding to different phases [start (69.63±3.08 min.), deepest (100.88±3.25 min.) and end (155.88±9.65 min.) points] of the hypothermia. Blood samples have been collected by intracardiac method, from the rats of the hypothermia and the control groups (0.09% salin, 0.5 ml/kg, ip). Analyses (ELISA) have been performed on the blood samples to determine the levels of hypothalamic-pituitary-thyroid (HPT) axis hormones [thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4)], hypothalamic-pituitary-adrenal (HPA) axis hormones [corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT)], leptin (Lep) hormone and erythropoietin (EPO) hormone. The control and hypothermia groups have been statistically compared (via ANOVA) with regard to the determined hormone levels and the significance of the changes (increase/decrease) between groups have been determined. As a result of the comparison to the control group, TSH, CRH, and EPO, the hormone levels change insignificant, the changes in the increase of FT4 (start point) hormone level and the changes in the decrease of FT3 (the deepest and end points), ACTH (start point), CORT (the deepest point), and Lep (end point) hormone levels have been found to be statistically significant (P<0.05). The hypothermia groups for which the hormonal changes are significant (P<0.05), have been treated with SC560 (1 mg/kg, sk) which is known to block both the hypothermia and the stimulation of the tumor necrosis factor-alpha (TNF-?). Hormonal analyses have been performed on the blood samples collected in the time intervals corresponding to the phases of the hypothermia induced by LPS. The results of these analyses have been statistically compared to the control group. As a result of the treatment, having determined that the LPS-induced hypothermia has been inhibited, the body temperatures at the deepest (?T: -0.57±0.04 ºC) and end (?T: 0.81±0.09 ºC) points of the hypothermia have been measured. At the same time, the significant (P<0.05) changes found in the hormonal levels have been normalized and vanished for the FT4, ACTH, CORT, and Lep hormone levels, while these changes have sustained for the FT3 hormone level. The results have led to thoughts that the treatment dose of SC-560 may have a critical significance on the LPS-induced hypothermia and the accompanying hormonal synchronization.
Açıklama
Polat, Hüseyin ( Aksaray, Yazar )
Anahtar Kelimeler
Sıçan, LPS, Hipotermi, HPT, HPA, Leptin, EPO, SC-560, Rat, LPS, Hypothermia
