Identification and technological characterization of endogenous yeast isolated from fermented black carrot juice, shalgam

dc.contributor.authorKahve, Halil İbrahim
dc.contributor.authorAkbulut, Mehmet
dc.contributor.authorÇoklar, Hacer
dc.date.accessioned2022-02-23T12:39:43Z
dc.date.available2022-02-23T12:39:43Z
dc.date.issued2022
dc.departmentMühendislik Fakültesi
dc.description.abstractShalgam is a fermented beverage obtained by lactic acid fermentation and its quality depends on flora. Therefore, it is essential to be able to characterize yeasts, in order to improve the quality and safety controls of final products. This study aimed to identify endogenous yeasts found during the fermentation of shalgam and to determine their technological properties. 172 yeast isolates were obtained and the DNA fingerprints were constituted by iPBS-PCR method. Sequencing of ITS regions of different groups revealed the species of Pichia kudriavzevii, P. fermentans, Saccharomyces cerevisiae, Candida oleophila, Kazachstania bulderi, and Geotrichum candidum. Interestingly, the prevalence of P. kudriavzevii in the flora increased during the fermentation, while the other species decreased. To determine the preliminary technological properties of yeasts, their development at different conditions was examined and 31 technologically superior strains were determined by Principal Component Analysis (PCA). Among these, 17 P. kudriavzevii strains had a vigorous fermentation rate and selected to assess their enzymatic activity. The isolates had diverse enzyme activities, including esterase, esterase lipase, and leucine arylamidase, which may have crucial roles for aroma formation. According to the results, P. kudriavzevii 3-3S9, 3-3S2, and 3-3Y1 strains were selected to be candidate starters for shalgam production.
dc.identifier.doi10.1016/j.lwt.2021.112823
dc.identifier.endpage-en_US
dc.identifier.issn0023-6438
dc.identifier.issue-en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage-en_US
dc.identifier.urihttps:/dx.doi.org/10.1016/j.lwt.2021.112823
dc.identifier.urihttps://hdl.handle.net/20.500.12451/9218
dc.identifier.volume154en_US
dc.identifier.wosWOS:000735306400005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAcademic Press
dc.relation.ispartofLWT
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectiPBS
dc.subjectShalgam
dc.subjectTechnological Characterization
dc.subjectYeast
dc.titleIdentification and technological characterization of endogenous yeast isolated from fermented black carrot juice, shalgam
dc.typeArticle

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