Chitosan Co-polymeric nanostructures for catalase immobilization

Basit Öğe Kaydı
dc.contributor.authorİnanan, Tülden
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:29Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:29Z
dc.date.issued2019
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstractCatalase is an industrial enzyme used in different areas including medical, textile, food and pharmaceutic industries. In the study, synthetic-natural copolymer in nanosize was synthesized using 2-hydroxyethyl methacrylate (HEMA) as a synthetic material and chitosan (CTS) as a natural material. Immobilized metal-chelate affinity chromatography (IMAC) material was prepared by chelating Cu(II) ions onto p(HEMA-CTS) nanostructures. Characterization studies such as Zeta sizer analysis, Fourier transform infra-red (FTIR) spectroscopy, scanning electron microscopy (SEM), and thermal gravimetric analysis (TGA) were performed during the preparation of IMAC nanostructures. The effects of some parameters (time, polymer amount, pH, initial catalase concentration, ionic strength and temperature) were identified and optimum immobilization conditions were determined as 75 min, 0.1 mg p(HEMA-CTS)-Cu, pH 6.0, and 25 degrees C. Maximum amount of immobilized catalase was detected as 11.29 g/g p(HEMA-CTS)-Cu. Immobilized catalase was desorbed successfully and it was detected that p(HEMA-CTS)-Cu nanostructures can be used repeatedly in catalase immobilization. Immobilization changed optimum temperature value from 37 degrees C to 25 degrees C while it did not change the optimum pH for catalase activity. Furthermore, it was detected that kinetic parameters of catalase changed by immobilization. Finally, operational and storage stabilities of immobilized catalase were investigated.
dc.description.sponsorshipAksaray University Research Fund [2016-016]
dc.description.sponsorshipThis work was supported by the Aksaray University Research Fund [Grant number 2016-016].
dc.identifier.doi10.1016/j.reactfunctpolym.2018.12.013
dc.identifier.endpage102en_US
dc.identifier.issn1381-5148
dc.identifier.issn1873-166X
dc.identifier.scopusqualityQ1
dc.identifier.startpage94en_US
dc.identifier.urihttps://doi.org/10.1016/j.reactfunctpolym.2018.12.013
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4604
dc.identifier.volume135en_US
dc.identifier.wosWOS:000458222500011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofReactive & Functional Polymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCatalase
dc.subjectImmobilization
dc.subjectCo-polymerization
dc.subjectChitosan
dc.subjectEnzyme Kinetics
dc.titleChitosan Co-polymeric nanostructures for catalase immobilization
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
inanan-tulden-2019.pdf
Boyut:
1.28 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu