Development and characterization of new polymorphic microsatellite loci for Trionyx triunguis (Testudines: Trionychidae) in the Mediterranean Basin

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dc.authoridBozdogan, Bulent -- 0000-0001-8846-7649;
dc.contributor.authorGüçlü, Özgür
dc.contributor.authorDurmuş, Salih Hakan
dc.contributor.authorCandan, Kamil
dc.contributor.authorBeşer, Nurettin
dc.contributor.authorTürkyılmaz, Süheyla
dc.contributor.authorYerli, Sedat
dc.contributor.authorBozdoğan, Bülent
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:19:14Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:19:14Z
dc.date.issued2015
dc.departmentGüzelyurt Meslek Yüksekokulu
dc.description.abstractThe Mediterranean subpopulation of T. triunguis has been listed by IUCN as critically endangered (European Reptile and Amphibian Specialist Group 1996, category CR C2A). There are very few studies for determination of distribution and population genetics of this species in the world. A total of 13 polymorphic microsatellite loci were isolated from 42 samples of T. triunguis by using biotinylated (GT)(10), (CT)(10), (GATA)(5)-enriched library. The range of observed heterozygosities was 0.000-0.833 with a mean of 0.274, and the range of expected heterozygosities was 0.081-0.607 with a mean of 0.374. It was found that four loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni correction analysis. There was no evidence for large allele dropout. Null alleles may be present at ttmed-6, ttmed-8, and ttmed-9 loci, as suggested by the general excess of homozygote for most allele size classes. Designed microsatellite loci could provide a powerful tool for the population structure and conservation genetics studies of the Nile soft-shelled turtle and for captive breeding programs or to detect species illegal trade.
dc.description.sponsorshipScientific and Technical Research Council of Turkey [TUBITAK-111T424]
dc.description.sponsorshipThis study was supported by the The Scientific and Technical Research Council of Turkey (TUBITAK-111T424 coded project). We wish to thank the The Ministry of Environment and Urban Planning and Ministry of Forest & Water Affairs for issuing a permit for tissue sampling.
dc.identifier.doi10.1163/15685381-00003003
dc.identifier.endpage324en_US
dc.identifier.issn0173-5373
dc.identifier.issn1568-5381
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage318en_US
dc.identifier.urihttps://doi.org/10.1163/15685381-00003003
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4970
dc.identifier.volume36en_US
dc.identifier.wosWOS:000360976600013
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherBrill Academic Publishers
dc.relation.ispartofAmphıbıa-Reptilia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMagnetic Beads
dc.subjectMicrosatellite
dc.subjectNile Soft-shelled Turtle
dc.subjectPolymorphism
dc.subjectTrionyx Triunguis
dc.titleDevelopment and characterization of new polymorphic microsatellite loci for Trionyx triunguis (Testudines: Trionychidae) in the Mediterranean Basin
dc.typeArticle

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