Extractıon, characterızatıon and inhibition sensitivity of ascorbate peroxidase from Kiwano (Cucumıs Metulıferus)

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dc.contributor.authorDenli, Zeynep
dc.contributor.authorDoğru, Mustafa Selim
dc.contributor.authorArabacı, Gülnur
dc.date.accessioned2020-04-09T11:38:31Z
dc.date.available2020-04-09T11:38:31Z
dc.date.issued2020
dc.departmentVeteriner Fakültesi
dc.descriptionDoğru, Mustafa Selim ( Aksaray, Yazar )
dc.description.abstractThe aim of the present study is to extract and characterize ascorbate peroxidase (APX) from kiwano (Cucumis metuliferus) and determine the effect of various inhibitors and metal ions on the enzyme activity. The enzyme activity was measured by the decrease in absorbance at 285 nm during 1 min with the spectrophotometric method. The optimum pH and temperature were found to be 6.2 and 30 degrees C respectively. Km and Vmax of APX extracted from kiwano were 0.2848 mM, 0.0035 U min-1 for aspartic acid (AsA), and 0.0649 mM, 0.0077 U min-1 for H2O2, respectively. APX extracted from kiwano was strongly inhibited by 2-nitrobenzoic acid (1.48% residual activity, 1 mM), KCN (13.3% residual activity, 1 mM), and NaN3 (25.9% residual activity, 1 mM), but less inhibited by L-cysteine, 2-mercaptoethanol, iodoacetamide, and EDTA. Analysis was performed for determining the effect of various metal ions, of which Fe2+ and Ba2+ activated the activity of APX at the concentrations of 1, 5 and 10 mM and inhibited completely by Cu2+ and Sn2+ at above 5 mM. APX extracted from kiwano lost its activity to some extent due to interacting with the rest of the metal ions. In this study, it was reported, for the first time, the extraction and biochemical characterization of the APX extracted from kiwano, which could be useful in potential applications.
dc.identifier.endpage1521en_US
dc.identifier.issue3en_US
dc.identifier.startpage1516en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/7518
dc.identifier.volume29en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherPSP - Parlar Scientific Publications
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAscorbate Peroxidase
dc.subjectEnzyme Kinetics
dc.subjectInhibitor Effect
dc.subjectMetal Ion Effect
dc.subjectKiwano
dc.subjectCucumis Metuliferus
dc.titleExtractıon, characterızatıon and inhibition sensitivity of ascorbate peroxidase from Kiwano (Cucumıs Metulıferus)
dc.typeArticle

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