A non-immunotoxic exopolysaccharide with anticancer effect in colorectal cancer isolated from Lacticaseibacillus rhamnosus ACS5 strain

dc.contributor.authorKadıoğlu, İlayda Selcen
dc.contributor.authorKaraduman Yeşildal, Tuğçe
dc.date.accessioned2024-07-25T08:21:36Z
dc.date.available2024-07-25T08:21:36Z
dc.date.issued2024
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractRecent studies demonstrated the potential of exopolysaccharides (EPS) produced by LAB (lactic acid bacteria), to be used in different fields. In our study, L-EPS-ACS5 from Lacticaseibacillus rhamnosus ACS5 strain isolated from Ayval & imath;k Cunda Sepet Cheese, one of the traditional cheeses of Turkey, showed antiproliferative effect in a timedependent manner in human colon cancer (HT -29) cell line compared to healthy control line (L929). This effect was also confirmed by colony formation analysis. Apoptotic changes were investigated at molecular level using qPCR, Western blot and it was determined that the relevant L-EPS induced apoptosis by increasing Bax , Caspase 3 , and 9 expression while reducing the Bcl-2 expression. These changes were confirmed by observations done at nuclear and cellular morphology level using a fluorescence microscope (Hoescht 33258) and TEM. In addition, the immunostimulatory activity of the relevant L-EPS was evaluated in terms of proliferation, phagocytosis ability and nitric oxide (NO) production parameters in the RAW 264.7 macrophage cell line. The increase in proliferative activity was not significant, and in parallel with these results, the differences in the control and application groups as a result of ' phagocytic activity and NO production analyzes ' were also not significant. However, L-EPS-ACS5 did not show immunotoxic/cytotoxic activity in macrophage cells (% viability >70). All these cellular and molecular evidences indicate that L-EPS-ACS5 may be a good alternative to synthetic anticancer agents with its anticancer effect and non-immunotoxic properties in colorectal cancer.
dc.identifier.doi10.1016/j.jrras.2024.100891
dc.identifier.issn1687-8507
dc.identifier.issue2en_US
dc.identifier.urihttps:/dx.doi.org/10.1016/j.jrras.2024.100891
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12219
dc.identifier.volume17en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Radiation Research and Applied Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLacticaseibacillus rhamnosus
dc.subjectExopolysaccharide
dc.subjectCell Culture
dc.subjectHT-29
dc.subjectAnticancer
dc.subjectRAW 264.7
dc.subjectImmunomodulatory Activity
dc.titleA non-immunotoxic exopolysaccharide with anticancer effect in colorectal cancer isolated from Lacticaseibacillus rhamnosus ACS5 strain
dc.typeArticle

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