Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials

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dc.authorid0000-0003-3835-304X
dc.authorid0000-0001-5458-9197
dc.authorid0000-0003-0900-4625
dc.authorid0000-0003-4612-4642
dc.authorid0000-0001-8954-5485
dc.authorid0000-0002-3954-3833
dc.contributor.authorChelgham, Abdelhakim
dc.contributor.authorSaadi, Abdelkader
dc.contributor.authorMerouane, Abdelaziz
dc.contributor.authorBensouici, Chawki
dc.contributor.authorÇçAKakmak, Yavuz Selim
dc.date.accessioned2025-07-10T10:25:26Z
dc.date.available2025-07-10T10:25:26Z
dc.date.issued2025
dc.departmentSabire Yazıcı Fen Edebiyat Fakültesi
dc.description.abstractPhlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts._HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry.
dc.identifier.doi10.21448/ijsm.1466689
dc.identifier.endpage396
dc.identifier.issn21486905
dc.identifier.issue2
dc.identifier.scopus105007070236
dc.identifier.startpage381
dc.identifier.urihttps://dx.doi.org/10.21448/ijsm.1466689
dc.identifier.urihttps://hdl.handle.net/20.500.12451/13235
dc.identifier.volume12
dc.indekslendigikaynakScopus
dc.institutionauthorÇakmak, Yavuz Selim
dc.institutionauthorid0000-0002-3954-3833
dc.language.isoen
dc.publisherPamukkale University
dc.relation.ispartofInternational Journal of Secondary Metabolite
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiological Activity
dc.subjectHerbal Medicine
dc.subjectHPLC-DAD
dc.subjectPhenolic Compounds
dc.subjectPhlomis Crinita
dc.titleExploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials
dc.typeArticle

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