Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine
Yükleniyor...
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hitit Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The surface imprinted technique was utilized in creating the synthetic lysozyme recep- tors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the arti- ficial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron mi- croscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
Açıklama
Anahtar Kelimeler
Lysozyme, Adsorption, Molecular imprinting, p(HEMA), Dopamine, Cryogel
Kaynak
Hittite Journal of Science and Engineering
WoS Q Değeri
Scopus Q Değeri
Cilt
10
Sayı
1
