Genetic polymorphism and cluster analyses based on the conserved DNAderived polymorphism markers in the selected Harumanis and non-Harumanis mango varieties
Loading...
Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Rynnye Lyan Resources
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Various mango varieties have been cultivated in Malaysia for decades and the fruit plays a significant impact on trading nationwide. Harumanis is the most outstanding mango variety in terms of taste and quality which led to a premium price of up to 8.57 USD per kilogram. This has steered fraud in substituting with cheaper mango varieties such as Tong Dam and Susu due to similar morphological features. Morphological characteristics are commonly used to differentiate Harumanis mango from other varieties although it is inefficient, less stable, and affected by environmental factors. This research aimed to evaluate the genetic polymorphism in three mango varieties and assess the potential of conserved-DNA derived polymorphism (CDDP) as a DNA marker in differentiating Harumanis and non-Harumanis mango samples. A total of fifteen Harumanis and non-Harumanis mango samples were studied. A total of 371 bands were amplified by a set of six CDDP primers for fourteen mango leaf samples. The percentage of polymorphism observed for all six primers was higher than 65%. Primer WRKY-R1 showed the highest polymorphism percentage and polymorphism information content, which was 100% and 0.44, respectively, making it the most efficient CDDP primer to differentiate Harumanis and non-harumanis mango varieties in this study. Primer WRKY-F1 showed the highest value of resolving power at 8.57 with the highest number of loci was 15. The UPGMA dendrogram constructed based on CDDP data revealed fourteen samples were grouped into four major clusters with all different varieties forming their own clad. The study demonstrated that CDDP markers can be effectively used in the characterization of different mango genotypes and in genetic diversity analysis, facilitating the development of DNA fingerprinting of the leading Harumanis mango, as well as better management of mango fruit resources in Malaysia.
Description
Keywords
Harumanis Mango Genetic Polymorphism, Mangifera Indica, Polymerase Chain Reaction, Polymorphism Information Content, Resolving Power
Journal or Series
Food Research
WoS Q Value
Scopus Q Value
Q3
Volume
8
Issue
5
