Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method

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dc.authorid0000-0001-5949-4752
dc.authorid0000-0002-2778-8059
dc.authorid0000-0003-1585-3359
dc.authorid0000-0003-2209-7512
dc.authorid0000-0001-8579-3203
dc.authorid0000-0003-3118-1054
dc.contributor.authorAkçakavak, Gökhan
dc.contributor.authorKarataş, Özhan
dc.contributor.authorTural, Ayşenur
dc.contributor.authorDağar, Osman
dc.contributor.authorDoğan, Osman
dc.contributor.authorTuzcu, Mehmet
dc.date.accessioned2024-10-03T12:40:40Z
dc.date.available2024-10-03T12:40:40Z
dc.date.issued2024
dc.departmentVeteriner Fakültesi
dc.description.abstractWhite muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of the disease. This study aimed to immunohistochemically evaluate local protein expressions of Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related protein 1A/1B-light chain 3 beta (LC3B) in WMD. The material of the study consisted of 15 WMD, and 6 healthy lamb heart samples. The heart tissues of the autopsied lambs were subjected to routine tissue processing and paraffin blocks were obtained. Then, it was stained with Hematoxylin-Eosin and immunohistochemical methods. Control group lambs had normal macroscopic appearance. Macroscopically, hyaline degeneration and zenker’s necrosis, calcification areas were observed in WMD tissues. Microscopically, degenerative and necrotic muscle fibers, calcification areas, fibrosis, mononuclear cell infiltrates and macrophage infiltrates were detected in WMD heart tissues. Immunohistochemically, significant increases were detected in IGF-1 (p<0.001), LC3B (p<0.001) and NF-kB (p<0.05) in the WMD group compared to the control group. Immunoreactivity in the relevant primers was detected commonly in degenerative and necrotic muscle fibers. In addition, occasional immunoreactivity was observed in the relevant primers in inflammatory cell infiltrates. In conclusion, NF-kB, IGF-1 and LC3B protein expressions were evaluated immunohistochemically for the first time in lambs with WMD. Our findings show that IGF-1 and LC3B proteins are highly expressed in heart tissue in WMD. Additionally, it is possible to say that IGF-1 and LC3B can be used in the diagnosis of WMD.
dc.identifier.doi10.31797/vetbio.1449118
dc.identifier.endpage86en_US
dc.identifier.issn2548-1150
dc.identifier.issue2en_US
dc.identifier.startpage79en_US
dc.identifier.urihttps:/dx.doi.org/ 10.31797/vetbio.1449118
dc.identifier.urihttps://hdl.handle.net/20.500.12451/12520
dc.identifier.volume9en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherİlker ÇAMKERTEN
dc.relation.ispartofJournal of Advances in Vetbio Science and Techniques
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHistopathology
dc.subjectIGF-1
dc.subjectLC3B
dc.subjectNF-kB
dc.subjectWhite Muscle Disease
dc.titleDetermination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method
dc.typeArticle

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