Arşiv logosu
  • Türkçe
  • English
  • Giriş
    Yeni kullanıcı mısınız? Kayıt için tıklayın. Şifrenizi mi unuttunuz?
Arşiv logosu
  • Koleksiyonlar
  • Sistem İçeriği
  • Analiz
  • Talep/Soru
  • Türkçe
  • English
  • Giriş
    Yeni kullanıcı mısınız? Kayıt için tıklayın. Şifrenizi mi unuttunuz?
  1. Ana Sayfa
  2. Yazara Göre Listele

Yazar "Tuncer, Pürhan Barbaros" seçeneğine göre listele

Listeleniyor 1 - 17 / 17
Sayfa Başına Sonuç
Sıralama seçenekleri
  • Yükleniyor...
    Küçük Resim
    Öğe
    Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull
    (Wiley, 2015) Sarıözkan, Serpil; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Bucak, Mustafa Numan; Cantürk, Fazile; Eken, Ayşe
    The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5mm), hyaluronan (0.25, 1mgml(-1)) and fetuin (5, 10mgml(-1)) in the freezing of Brown Swiss bull semen. The best percentages of CASA motilities were achieved with 10mgml(-1) of fetuin and 2.5mm of cysteamine. For sperm morphology, 10mgml(-1) of fetuin and 2.5mm of cysteamine had better protective effects (P<0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5mgml(-1) of fetuin, were higher than those of the control group (P<0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25mgml(-1) of hyaluronan, and 2.5 and 7.5mm of cysteamine led to lower rates of spermatozoa with damaged DNA, compared with the control group (P<0.001). The maintenance of superoxide dismutase and glutathione peroxidase antioxidant activities following freeze-thawing with 2.5 and 7.5mm of cysteamine and 10mgml(-1) of fetuin was demonstrated to be at a higher level in comparison with the control group (P<0.001). Malondialdehyde formation was found to be lower in the groups supplemented with 0.25mgml(-1) of hyaluronan and 7.5mm of cysteamine after the freeze-thawing process (P<0.001).
  • Yükleniyor...
    Küçük Resim
    Öğe
    Can linoleic acid improve the quality of frozen thawed bull sperm?
    (Cryo-Letters, 2014) Büyükleblebici, Serhat; Taşdemir, Umut; Tuncer, Pürhan Barbaros; Durmaz, Emre; Özgürtaş, Taner; Büyükleblebici, Olga; Coşkun, Erdem; Gürcan, İsmail Safa
    BACKGROUND: Cryopreservation is known to have a detrimental effect on the motility, viability and membrane integrity of sperm cells. OBJECTIVE: The aim of this study was to investigate the effect of various amount of linoleic acid supplementation to the Tris extender, on bull sperm parameters, DNA integrity and oxidative stress after freeze-thawing. METHODS: Ejaculates were split into five aliquots and extended to a final concentration of 18x10 6 spermatozoa/ml with the base extender containing different doses of linoleic acid 0.125 ml, (L125); 0.250 ml (L250); 0.5 ml (L500), 1 ml (L1000) and no additive (control; L0). The extended samples were equilibrated slowly to 4°C for 4 h and then froze using a digital freezing machine. Frozen straws were thawed individually in water bath at 37°C for 30 s to analyse progressive motility and sperm motion characteristics as well as membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. DNA damage was evaluated by Comet Assay. RESULT: The addition of various linoleic acid did not improve the sperm subjective, CASA and progressive motilities, sperm motility characteristics and DNA integrity (P>0.05). L500 exhibited the greatest values for membrane integrity than that of the other groups (P<0.001). All supplementation groups led to lower percentages of tail abnormalities in comparison to the control (P<0.001). L500 and L1000 significantly decreased total abnormalities. In conclusion, our findings showed that L500 linoleic acid supplementation in semen extender was of great beneficial effect on frozen-thawed bull semen in terms of morphology and plasma membrane integrity. © CryoLetters.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders
    (Academic Press, 2014) Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Bucak, Mustafa Numan; Taşdemir, Umut; Eken, Ayşe; Büyükleblebici, Olga; Durmaz, Emre; Sarıözkan, Serpil; Endirlik, Burcu Ünlü
    There are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5 mM or sucrose 25 mM, and control (without additives) was cooled to 4 degrees C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P > 0.05). However, EG3 + S yielded the greatest percentages of the total abnormality (P < 0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P < 0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P < 0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages the tail intensity and tail moment (P < 0.05). There were no significant differences observed in non-return rates among all treatment groups (P > 0.05). (C) 2014 Elsevier Inc. All rights reserved.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Comparison of cryoprotective effects of lycopene and cysteamine in different cryoprotectants on bull semen and fertility results
    (Wiley-Blackwell, 2014) Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Eken, Ayşe; Taşdemir, Umut; Durmaz, Emre; Büyükleblebici, Olga; Çoşkun, Erdem
    Contents The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty-four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5mm or lycopene 500g/ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol with lycopene resulted in a better rate of CASA progressive motility compared with that of other groups. Generally, all the lycopene groups signed better protective effects on acrosome and total morphology than the other groups. Glycerol 7% and 3% ethylene glycol with lycopene groups yielded to slight higher percentages of membrane integrity assessed by HOST than that of the other groups, but 7% glycerol with cysteamine and 3% ethylene glycol with cysteamine showed the worst percentages of membrane integrity. Glycerol 7% and 5% glycerol with lycopene gave rise to a higher value of VAP, VSL and VCL compared with that of the other groups. On the contrary, adding to 5% glycerol with cysteamine showed negative effect for VAP, VSL, VCL and ALH values. All cryoprotectant groups with lycopene decreased chromatin damage than the other groups. Ethylene glycol 3% led to lower non-return rates of inseminated cows. However, this result was not considered to be statistically important.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Cryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results
    (Elsevier Science Bv., 2014) Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Bucak, Mustafa Numan; Eken, Ayşe; Sarıözkan, Serpil; Taşdemir, Umut; Endirlik, Burcu Ünlü
    The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5 mM C or 25 mM T, and control (without additives) was cooled to 4 degrees C and frozen in 0.25 ml French straws. The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatment with 25 mM Tin 3% EG beneficially effected acrosome morphology as compared with the other groups. Also, treatment with 3% EG with 25 mM T and 5% EG resulted in a greater rate of total abnormalities. Treatment with 3% G yielded a slightly greater percentage of membrane integrity by Hypo-Osmotic Swelling Test (HOST) assessment than that of the other groups. Treatment with 3% EG with 5 mM C resulted in the greatest concentration of malondialdehyde (MDA). The glutathione peroxidase (GPx) antioxidant activity was increased in the C-treatment groups when compared to the other groups. Treatment with 5% EG and 5 mM C resulted in less chromatin damage and detrimental impacts on tail moment. Treatment with 5% EG led to greater non-return rates of inseminated cows. However, this result was not considered to be statistically important.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Cryopreservation of rainbow trout oncorhynchus mykiss spermatozoa: effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility
    (Academic Press, 2014) Kutluyer, Filiz; Kayim, Murathan; Ogretmen, Fatih; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros
    In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Effect of ?--Lipoic acid supplementation to extender on quality of frozen-thawed bull semen
    (Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015) Büyükleblebici, Olga; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Özgürtaş, Taner; Taşdemir, Umut
    This study was conducted to investigate the possible protective effect of ?--lipoic acid (ALA) supplementation to extender on damages in the quality of bull semen elicited by freze-thawing process-induced oxidative stres. Ejaculates were collected via an artificial vagina from the bulls at once a week. Then they were split into five aliquots and extended with the Tris base extender containing different doses of ALA, except control. The extended samples were equilibrated slowly to 4°C for 4 h and then frozen using a digital freezing machine. Frozen straws were thawed to analyse progressive motility and sperm motility characteristics as well as plasma membrane integrity. Biochemical assays were performed in a spectrophotometer using commercial kits. Treatment of extender with ALA groups caused a significant decrease in percentages of post-thaw sperm CASA, progressive motilities and sperm motility characteristics such as VAP, VSL and VCL except ALA0.5. Besides ALA0.5 showed lower percentages of acrosome and total abnormalities in comparison to the control. In conclusion, findings generated here showed that ALA0.5 supplementation in Tris based semen extender was of great beneficial effect on frozen-thawed bull semen in terms of morphology and plasma membrane integrity
  • Yükleniyor...
    Küçük Resim
    Öğe
    Effects of different doses of trehalose supplementation in egg yolk extender in frozen-thawed Angora buck semen
    (2013) Tuncer, Pürhan Barbaros; Taşdemir, Umut; Büyükleblebici, Serhat; Özgürtaş, Taner; Coşkun, Erdem; Erol, Halil; Aydın, Fevzi Nuri; Gürcan, İsmail Safa
    Few studies have been done on the effects of trehalose supplementation in the cryopreservation of Angora buck semen. The objective of present study was to investigate the effects of the addition of trehalose at different doses in semen extenders, on in vitro semen quality parameters, anti-oxidant enzymes activities and DNA damage after the freeze-thaw process in Angora buck semen. Semen samples from 5 mature Angora bucks (3 and 4 years of age) were used in this study. The bucks, belonging to the Livestock Central Research Institute were maintained under uniform breeding condition. A total number of 40 ejaculates were collected twice a week from the bucks using an artificial vagina, during the breeding season and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 7 equal aliquots and diluted (37. °C) with base extenders supplemented with the trehalose (12.5, 25, 50, 75, 100 and 150. mM), and a base extender with no additives (control). Diluted samples were aspirated into 0.25. ml French straws, and equilibrated at 5. °C for 4. h and then were frozen at a digital freezing machine. The freezing extender supplemented with 50. mM trehalose led to the greatest percentages of CASA motility (53.6. ± 4.69), in comparison to the other groups after the freeze-thawing process (P < 0.05). The addition of different doses of trehalose did not provide any significant effect on the percentages of post-thaw sperm motion characteristics (VAP, VSL and LIN), compared to the control (P > 0.05). The freezing extender with 150. mM trehalose group led to the highest percentages of acrosome abnormalities (P < 0.05) and 50. mM trehalose group had the lowest percentages of total abnormalities (P < 0.001), in comparison to the others. There were no significance differences in the DNA integrity among treatment groups (P > 0.05). The different doses of trehalose did not show any effectiveness on the maintenance GPx, LPO, GSH, CAT and total antioxidant activities, when compared to the control (P > 0.05). Therefore, the additions of 50. mM and 75. mM doses of trehalose will be useful in increasing post thaw motility on Angora buck semen. © 2013 Elsevier B.V.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Effects of various antioxidants on cryopreserved bull sperm quality
    (Kafkas Üniversitesi, 2014) Taşdemir, Umut; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Özgürtaş, Taner; Durmaz, Emre; Büyükleblebici, Olga
    The objective of this study was to assess the effects of antioxidant supplement (A), fetuin (F), aminoacid (AS) and cysteine (CY) on the sperm parameters, plasma membrane integrity, chromatin damage and antioxidant activities after freeze-thawing. Ejaculates were split into five aliquots and extended to a final concentration of 15x106 spermatozoa/ml with the Tris base extender containing 0.5 ml A, 2 mg/ml F, 13% AS, 5 mM CY andadditive (C). The extended samples were equlibrated slowly to 4°C during 4 h and then frozen using a digital freezing machine.. Frozen straws were thawed individually in water bath at 37°C for 30 s to analyse progressive motility and sperm motion characteristics as well as membrane integrityBiochemical assays were performed in a spectrophotometer using commercial kits. Chromatin damage was evaluated by Comet Assay. A, F, AS anddid not show better result on the percentages of post-thaw sperm motilities. CY exhibited the greatest value of plasma membrane integrity (P<0.05)Total abnormalities were greater in C and F (17.5±0.57%; 15.5±1.98%, respectively; P<0.05). F had greater chromatin damage results (P<0.05).activity was affected by type of antioxidant, notably CY yielded the lowest results when compared to the other groups (P<0.05). In conclusion, although using antioxidants does not have any influence on the sperm motility after thawing, A, AS and CY cause reduction at abnormal spermatozoa;exhibits the greatest cryoprotective activity on plasma membrane integrity and F caused an increase at chromatin damage.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters
    (Academic Press, 2013) Taşdemir, Umut; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Coşkun, Erdem; Özgürtaş, Taner; Aydın, Fevzi Nuri; Büyükleblebici, Olga; Gürcan, İsmail Safa
    The objectives of this study was to compare the effects of type and concentration of cryoprotectants glycerol (G), ethylene glycol (EG) and dimethyl sulfoxide (DMSO) on the plasma membrane and DNA integrity as well as antioxidant activity of cryopreserved Eastern Anatolian red bull sperm. Ejaculates were collected from the three bulls using an artificial vagina twice a week. The ejaculates were pooled to increase the semen volume for replication and to eliminate variability among the evaluated samples. The pooled ejaculates were also split into seven equal experimental groups and diluted with the modified base extender to a final spermatozoa concentration of 15 x 10(6) /ml. The extended samples were cooled slowly to 4 degrees C and equilibrated for 4 h. They were then loaded into 0.25 ml French straws and frozen using a digital freezing machine at 3 programmed rates: -3 degrees C/min from +4 degrees C to -10 degrees C, -40 degrees C/min from -10 degrees C to -100 degrees C, and -20 degrees C/min from -100 degrees C to -140 degrees C. Thereafter, the straws were plunged into liquid nitrogen at -196 degrees C. Frozen straws were thawed individually at 37 degrees C for 30 s in a water bath to analyse progressive motility and sperm motion characteristics as well as membrane integrity using hypo-osmotic swelling test. Biochemical assays were performed in a spectrophotometer using commercial kits. DNA damage was evaluated by Comet Assay using Image Analysis System. 6% G exhibited the greatest percentages of CASA (43.7 +/- 2.92%) and progressive (26.4 +/- 2.64%) motilities when compared to the other groups (P < 0.001). 6% G and 6% EG showed the greatest values of preserved membrane integrity (P < 0.001). 6% DMSO and 3% EG + 3% DMSO resulted in greater chromatin damage than the other groups (P < 0.001). The antioxidant activities of GPx, GSH, and CAT as well as the total antioxidant activity were affected by the type of cryoprotectant; notably, 2% G + 2% EG + 2% DMSO yielded the lowest activities when compared to the other groups (P < 0.001). In conclusion, no advantages were found in using EG or DMSO to replace G in bull sperm cryopreservation. Freezing with cryoprotectant 6% G yielded the best post-thaw sperm characteristics for Eastern Anatolian Red bull spermatozoa. (c) 2012 Elsevier Inc. All rights reserved.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen
    (Acta Veterinaria Brno, 2023) Güngör, Şükrü; Yeni, Deniz; İnanç, Muhammed Enes; Türkmen, Ruhi; Tuncer, Pürhan Barbaros; Gülhan, Mehmet Fuat; Avdatek, Fatih; Taşdemir, Umut
    This study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 ?g/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (?196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 ?g/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had higher DNA integrity than the other groups (P < 0.05). This finding was supported by low malondialdehyde levels (P < 0.05). Especially the 25 ?g/ml dose of JB was found to have a remedial effect on DNA integrity, as well as increasing progressive and total motility rates. This study has revealed new information on the effects of JB on bull semen cryopreservation. Juniper berry oil may be used as an antioxidant in bull semen freezing.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen
    (Elsevier, 2015) Sarıözkan, Serpil; Bucak, Mustafa Numan; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Eken, Ayşe; Akay, Cemal
    It was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P > 0.05). Sperm acrosome and total abnormalities were similar in all groups (P > 0.05). Groups M (hyaluronan + fetuin) and H (hyaluronan) displayed a higher rate of sperm membrane integrity, compared to that of Group C (control) (P < 0.01). According to the results of the comet assay, the lowest percentage of sperm with damaged DNA was achieved in Group H, when compared to all of the experimental groups (P < 0.01). Furthermore, all of the additives resulted in a lower rate of sperm with damaged DNA than that of the controls, and thus, reduced DNA damage (P < 0.01). For pregnancy rates, there were no significant differences between the extender groups (P > 0.05). MDA formation was found to be lower in Groups M and F (P < 0.01). In Group M, SOD activity was determined to have significantly increased (23.61 +/- 5.62 U/ml) compared to the other groups (P < 0.01). All experimental groups had a GSH-Px activity higher than that of the control group (P < 0.01). Crown Copyright (C) 2015 Published by Elsevier Inc. All rights reserved.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Rasyon çinko miktarının boğalarda sperma miktar ve kalitesine etkisi
    (Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, 2014) Ünay, Engin; Yaman, Sema; Kinet, Hüseyin; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Karakaş, Vedat
    Araştırmada, hayvan materyali olarak, Hayvancılık Merkez Araştırma Enstitüsünde, dondurulmuş sperma üretimi yapılan 9 baş Siyah Alaca ırkı boğa kullanılmıştır. Araştırma, karma yemde 40 mg/kg çinko içeren kontrol grubu ve kontrol grubunda kullanan karma yeme ilave olarak 110mg/kg çinko (toplam 150 mg/kg Zn içeren 1. grup) ve 160 mg/kg (toplam 200 mg/kg Zn içeren 2. grup) çinko eklenen 3 farklı muamele grubundan oluşmuştur. Her gruba tesadüfi olarak 3er baş boğa dağıtılmıştır. Çinko preparatı olarak, kontrol grubu ve diğer iki muamele grubunda organik çinko bileşiği Bioplex Zinc (Aminoasit hidratin çinko şelat, Alltech) kullanılmıştır. Deneme başlangıcında, 60 gün ön yemleme yapılmıştır. Bu süre sonunda boğalardan suni vajen yöntemiyle 5 hafta boyunca, haftada 2 defa 2’şer ejakülat sperma alınmıştır. Sulandırılan spermalar, ekilibrasyondan sonra 0.25 ml’lik payetlere çekilerek, kontrollü dondurma cihazında dondurulup sıvı azot içinde saklanmıştır. Nativ spermatolojik değerlendirmeler aynı gün, çözüm sonu değerlendirmeleri ise 24 saatlik dondurma sürecinden sonra payetler 37°C’de 30 saniye süreyle çözdürülerek değerlendirilmiştir. Çalışmada sperma miktarı yönünden kontrol grubu ile 200 mg/kg çinko içeren 2. grup arasından fark önemsiz, kontrol grubu ile 150 mg/kg çinko içeren 1. grup arasındaki fark önemli (P<0,05) bulunmuştur. Motilite oranı, mass aktivite değeri ve yoğunluk yönünden kontrol grubu ile 1. ve 2. gruplar arasındaki fark önemli bulunmuştur (P<0,05). Sperma yoğunluğu bakımından en iyi sonuç 1. gruptan alınmıştır. Çözüm sonu en iyi motilite değeri ise 2. grupta elde edilmiştir. Damızlık boğaların beslenmesinde, karma yemde 40 mg/kg çinko (NRC 2001) kullanılması yerine, 150 veya 200 mg/ kg çinko kullanılmasının sperma miktarı ve kalitesi üzerine olumlu etki yaptığı kanaatine varılmıştır.
  • [ X ]
    Öğe
    Supplementation of rosmarinic acid has reduced oxidative stress on bull spermatozoa followİng the freeze thawing process
    (Cryo-Letters, 2018) Yeni, Deniz; İnanç, Muhammed Enes; Avdatek, Fatih; Tuncer, Pürhan Barbaros; Çil, Beste; Türkmen, R.; Taşdemir, Umut
    BACKGROUND: Cryopreservation has a side effect on the motility, chromatin integrity and viability of sperm cells. OBJECTIVE: The present study investigated the effects of supplementation with rosmarinic acid (RA) Tris extender on sperm quality parameters, plasma and acrosome membrane damage, antioxidant enzyme activity and chromatin integrity following the freeze thawing process on bull spermatozoa. MATERIALS AND METHODS: Ejaculates were split into five aliquots and diluted to a final concentration of 15x106 spermatozoa/ml with the Tris extender containing RA (25, 50, 100 and 200 µg/ml) and (control) and then frozen at a controlled rate. RESULT: Treatments did not give better results on the percentages of sperm progressive, total motility and sperm motion characters (P>0.05); however, RA25 and RA50 exhibited favourable chromatin integrity. In conclusion, RA25 and RA50 increased total antioxidant activity. As a consequence, the amount of MDA and chromatin damage were reduced in sperm cells. © CryoLetters.
  • Yükleniyor...
    Küçük Resim
    Öğe
    The comparison of three different cryoprotectants in cryopreservation of Angora goat semen
    (Kafkas Üniversitesi, 2014) Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Taşdemir, Umut; Özgürtaş, Taner; Durmaz, Emre; Büyükleblebici, Olga
    The objective of this study was to evaluate glycerol (G), ethylene glycol (EG) and dimethylsulfoxide (DMSO) which were used two different doses on in vitro semen parameters, antioxidant enzymes activities and DNA damage after the freeze-thaw process in Angora goat semen. Semen samples from 5 mature Angora goats were used in this study. A total number of 40 ejaculates were collected twice a week from the goats using an artificial vagina and the semen pooled to minimize individual variation. Each pooled ejaculate was split into 6 equal aliquots and diluted with tris base extenders supplemented with two different doses of cryoprotectants (G 3%, 6%; EG 3%, 6%; DMSO 3%, 6%). G 3% and 6% was added as a cryoprotectant had better CASA motility (P<0.01) and progressive motility (P<0.001) values when compared to EG and DMSO groups. On the other hand, EG 6% showed the best values of preserved membrane integrity (P<0.01). The evaluation of CASA sperm motions parameters, adverse effects were procured in the groups with DMSO groups when compared to the other groups (P<0.05; P<0.001). G 6% group was the greatest VAP, VSL and VCL values than the other groups (P<0.05; P<0.001). DNA damage was not affected by supplemented different doses of cryoprotectants as well as antioxidant activity (P>0.05). In conclusion, no advantages were found in using EG or DMSO to replace G for freezing of Angora goat sperm
  • Yükleniyor...
    Küçük Resim
    Öğe
    The effects of different antioxidants on post-thaw microscopic and oxidative stress parameters in the cryopreservation of Brown-Swiss Bull Semen
    (Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2016) Büyükleblebici, Olga; Büyükleblebici, Serhat; Taşdemir, Umut; Tuncer, Pürhan Barbaros
    The aim of this study was to research the effects of four antioxidants that had been added to semen diluents, on spermatological parameters, anti-oxidant enzymes activities and DNA integrity after the freeze-thaw procedures in Brown-Swiss bull semen. A total of 24 ejaculates were collected from three bulls. Each ejaculate was divided into five equal parts and they diluted to the Tris + 25 mM trehalose base extender containing fetuin 1 mg/ml, dithioerithritol 1 mM, cysteamine 4 mM or linoleic acid 0.5 ml/50 ml, and no additives (control). Group dithioerithritol and linoleic acid showed the higher rates of CASA progressive motility, CASA sperm motility (P<0.05) compared with the other groups. Group linoleic acid provided the better protective effect for acrosome and total abnormalities (P<0.01, P<0.05; respectively). Also, group dithioerithritol showed the highest values for the HOS test (P<0.01). In the comet test, group dithioerithritol enhanced tail with lesser chromatin damage than the other groups (P<0.05). Supplementation of dithioerithritol significantly affected the GSH activity (P<0.01). Also groups dithioerithritol and linoleic acid gave higher CAT values than the other groups (P<0.05). Additionally, supplementation of fetuin and cysteamine showed the lowest total antioxidant activity value (P<0.05). In conclusion, we may said that, the addition of antioxidants, specially dithioerithritol and linoleic acid, to added with 25 mM trehalose Tris extender improve post-thaw sperm parameters.
  • Yükleniyor...
    Küçük Resim
    Öğe
    Use of infrared thermography during ejaculation process and Its link with semen quality and freezability in dogs
    (MDPI, 2021) Tekin, Koray; İnanç, Muhammed Enes; Özen, Doğukan; Çil, Beste; Olğaç, Kemal Tuna; Yılmaz, Burak; Taşdemir, Umut; Tuncer, Pürhan Barbaros; Büyükleblebici, Serhat; Daşkın, Ali; Uysal, Ongun; Stelletta, Calogero
    This study aimed to describe the thermal variation of external reproductive tracts during ejaculation in relation to sperm quality in dogs. Forty-six adult fertile dogs were monitored using a thermal camera before, during and after the semen collection, taking into account penile and scrotal temperatures as reproductive thermal patterns while eye and perianal temperatures were recorded as complementary thermal patterns of behavioral response. The parameters were classified depending on age (?4 years and >4 years), body weight (BW) (?75 kg and >75 kg), sperm concentration (CON) (?300 million and >300 million), total testicular volume (TTV) (?600 cm3 and >600 cm3) and total ejaculation time (TET) (?800 s and >800 s) of the animals from which semen was collected successfully. Heavier males (p < 0.05) that have more consistent testicles (p < 0.01) as well as quicker ejaculate responders (p < 0.001) and lower scrotal temperature had better semen (? motility) freezability. The lower eye temperature prior to the ejaculation (p < 0.01), lower scrotal temperature following ejaculation (p < 0.01), and conversely, higher penile temperature during the ejaculation (p < 0.001) had a higher sperm concentration. Furthermore, the sperm freezability was negatively correlated with total ejaculation time (r = -0.39, p < 0.05) and sperm abnormalities were lower in the ejaculate of dogs having a higher temperature of the scrotum, bulbus and penis. In conclusion, infrared monitoring throughout semen collection in dogs can provide information on behavioral reactions during human manipulation, as well as semen quality and testicular functionality.

| Aksaray Üniversitesi | Kütüphane | Açık Bilim Politikası | Açık Erişim Politikası | Rehber | OAI-PMH |

Bu site Creative Commons Alıntı-Gayri Ticari-Türetilemez 4.0 Uluslararası Lisansı ile korunmaktadır.


Aksaray Üniversitesi Kütüphane ve Dokümantasyon Daire Başkanlığı, Aksaray, TÜRKİYE
İçerikte herhangi bir hata görürseniz lütfen bize bildirin

Powered by İdeal DSpace

DSpace yazılımı telif hakkı © 2002-2025 LYRASIS

  • Çerez Ayarları
  • Gizlilik Politikası
  • Son Kullanıcı Sözleşmesi
  • Geri Bildirim