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Öğe 2-[(2E)-2-(3-chloro-2-fluorobenzylidene)hydrazinyl]pyridine: Synthesis, spectroscopic, structural properties, biological activity and theoretical analysis(Elsevier, 2021) Özçelik, Nefise; Tunç, Tuncay; Çelik, Raziye Çatak; Erzengin, Mahmut; Özışık, HacıThe newly synthesized hydrazone derivative: 2 [(2E)-2-(3-chloro-2-fluorobenzylidene)hydrazinyl] pyridine was synthesized. The characterization of the title compound was carried out by elemental analysis, FT-IR, H-1 NMR, C-13 NMR and UV-VIS measurements. The crystal structure was determined by the single crystal X-ray diffraction (XRD) method. In addition, the title compound was also subjected to its possible antioxidant activity with free radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals using 3,5-di-tert-butyl-4-hydroxy-toluene (BHT) as standard antioxidant. The theoratical structural calculations were carried out by the density functional theory using the B3LYP method with 6-311++G(2d,2p) basis set. The theoretical and experimental results obtained were examined and compared.Öğe A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles(Elsevier Ltd., 2011) Erzengin, Mahmut; Ünlü, Nuri; Odabaşı, MehmetThe aim of this study is to prepare supermacroporous cryogels embedded with Cu2+-attached sporopollenin particles (Cu2+-ASP) having large surface area for high protein adsorption capacity. Super-macroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Cu2+-ASP was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA). Firstly, Cu2+ ions were attached to sporopollenin particles (SP), then the supermacroporous PHEMA cryogel with embedded Cu2+-ASP was produced by free radical polymerization using N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Embedded particles (10 mg) in PHEMA-based cryogel column were used in the adsorption/desorption of HSA from aqueous solutions. Optimum conditions of adsorption experiments were performed at pH 8.0 phosphate buffer, with flow rate of 0.5 mL/min, and at 5 degrees C. The maximum amount of HSA adsorption from aqueous solution was very high (677.4 mg/g SP) with initial concentration 6 mg/mL It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Cu2+-ASP in PHEMA cryogel without significant loss of adsorption capacity. (C) 2010 Elsevier B.V. All rights reserved.Öğe A novel matrix for hydrophobic interaction chromatography and its application in lysozyme adsorption(Polskie Towarzystwo Biochemiczne, 2014) Gedikli, Mehmet; Ceylan, Şeyda; Erzengin, Mahmut; Odabaşı, MehmetA novel 1-naphthylamine (NA) coupled poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(L)-histidine methyl ester) [NA-PHEMAH] supermacroporous monolithic hydrophobic cryogel was prepared via covalent coupling of NA to PHEMAH for adsorption of lysozyme from aqueous solution. Firstly, PHEMAH monolithic cryogel was prepared by radical cryocopolymerization of HEMA with MAH as a functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as a crosslinker directly in a plastic syringe, and then NA molecules were covalently attached to the imidazole rings of MAH groups of the polymeric structure. The prepared, NA-PHEMAH, supermacroporous monolithic hydrophobic cryogel was characterized by scanning electron microscopy (SEM). The effects of initial lysozyme concentration, pH, salt type, temperature and flow rate on the adsorption efficiency of monolithic hydrophobic cryogel were studied in a column system. The maximum amount of lysozyme adsorption from aqueous solution in phosphate buffer was 86.1 mg/g polymer at pH 8.0 with a flow rate of 1 mL/min. It was observed that lysozyme could be repeatedly adsorbed and desorbed with the NA-PHEMAH monolithic hydrophobic cryogel without significant loss of the adsorption capacity.Öğe Affinity purification and characterization of polyphenol oxidase from helianthus tuberosus L.(Hacettepe Üniversitesi, 2009) Erzengin, MahmutPolyphenol oxidase (PPO) of Jerusalem artichoke (Helianthus Tuberosus L.) tubers was purified using a Sepharose-4B-L-tyrosine-p-amino benzoic acid affinity gel. Both nativeand SDS-gel electrophoresis analyses of the purified PPO gave a single band (ca. 65 kDa based on SDS-PAGE), indicating that it is a monomer. The purified PPO showed activity towards diphenolic and triphenolic substrates but not with the monophenolic substrates, suggesting that it lacks monophenolase activity. The optimum temperature and pH values vary between 20-35oC and 5.0-8.0, respectively, depending on the substrate used; for catechol, the optimum temperature and pH values were found to be 20oC and 7.0, respectively. The purified enzyme was relatively stable at 40oC but unstable at higher temperatures. Furthermore, IC50 values for various inhibitors and inhibition modes were also determined using catechol as a substrate; ?-mercaptoethanol showed the strongest inhibition, followed by 2-mercapto benzothiazol, glutathione, L-cysteine and dithioerythritol, respectivelyÖğe Antimicrobial and Antioxidant Activities of Two Endemic Plants From Aksaray in Turkey(African Networks Ethnomedicines, 2013) Aşan Özüsağlam, Meltem; Önal Darılmaz, Derya; Erzengin, Mahmut; Tekşen, Mehtap; Karaman Erkul, SeherThis study was designed to examine the in vitro antimicrobial and antioxidant activities of the methanol, ethanol, water, n-hexane and dicholoromethane extracts of two Allium species (Allium scabriflorum and Allium tchihatschewii) which are endemic for the flora of Turkey. The antimicrobial efficiency of the plant was evaluated according to disc diffusion and microdilution broth methods. The antimicrobial test results showed that the extracts of A. scabriflorum and A. tchihatschewii showed varying degrees of antimicrobial activity on the tested microorganisms. The extracts were screened for their possible antioxidant activities by three complementary tests; DPPH free radical-scavenging, scavenging of hydrogen peroxide and metal chelating activity assays. All the extracts of A. scabriflorum and A. tchihatschewii exhibited lower DPPH free radical scavenging activity but higher metal chelating activity when compared to standards. The values of scavenging of hydrogen peroxide of the extracts were higher than the standards that of a-tocopherol, BHA, BHT and trolox, but close to that of ascorbic acid. In addition to the antioxidant activity of these plants, the total phenolic compounds and flavonoids were also measured in the extracts. The results presented here may suggest that the extracts of A. scabriflorum and A. tchihatschewii possess antimicrobial and antioxidant properties, and therefore, they can be used as a natural preservative ingredient in food and/or pharmaceutical industry.Öğe Antimicrobial and antioxidant activities of various extracts of endemic Crepis macropus Boiss & Heldr (Asteraceae)(Current Biology, 2011) Aşan Özüsağlam, Meltem; Karakoca, Kübra; Erzengin, Mahmut[Abstract Not Available]Öğe Antimicrobial and antioxidant activity of various solvent extracts of Salsola stenoptera wagenitz and Petrosimonia nigdeensis aellen (Chenopodiaceae) plants(Chiang Mai University, 2015) Aşan Özüsağlam, Meltem; Erzengin, Mahmut; Darılmaz, Derya Önal; Erkul, Seher Karaman; Tekşen, Mehtap; Karakoca, KübraThis study was conducted to examine the in vitro antimicrobial and antioxidant activities of various extracts (ethanol, methanol, n-hexane, dicholoromethane and water) prepared from the stem and leaf+fruit of Salsola stenoptera Wagenitz and Petrosimonia nigdeensis Aellen (Chenopodiaceae) plants. The antimicrobial activities of the plant extracts were tested according to agar well diffusion and microdilution broth methods. The extracts showed a strong antimicrobial activity and a broad antimicrobial spectrum for all tested microorganisms. Minimal bactericidal (MBC) or fungicidal (MFC) concentrations for microorganisms which were sensitive to the stem and fruit+leaf extracts of S. stenoptera and P. nigdeensis in the agar well diffusion assay were in the range of 5.63-180.00 mg/mL and 11.25-180.00 mg/mL, respectively. The extracts were also subjected to screening for their possible antioxidant activity with scavenging activity of DPPH radicals, hydrogen peroxide scavenging activity and chelating ability on ferrous ions. All tested extracts showed varying degrees of efficacy in each assay in a dose-dependent manner. In general, polar extracts exhibited stronger activities than non-polar extracts. The ethanol and n-hexane extracts of S. stenoptera and P. nigdeensis stem showed the highest scavenging abilitiy on DPPH radicals with the IC50 values of 29.84 µg/mL and 83.56 µg/mL, respectively. The total phenolic and flavonoid contents were also determined. The highest gallic acid equivalents (GAE) and quercetin equivalents (QE) values, for both plants, were 44.78 µg GAE/mg and 66.69 µg QE/mg, respectively. The results indicate that both plant species may have health benefits as sources of natural antioxidants and antimicrobial agents for use in functional foods and/or in pharmaceutical industry. © 2015, Chiang Mai University. All rights reserved.Öğe Antipsychotic agents screened as human carbonic anhydrase I and II inhibitors(Informa Healthcare, 2014) Erzengin, Mahmut; Bilen, Çiğdem; Ergün, Adem; Gencer, NahitThe antipsychotic drugs currently used to treat schizophrenia can be divided into two distinct classes, typical and atypical antipsychotics. Many drug molecules are enzyme inhibitors that bind reversibly or irreversibly to their target through intermolecular interactions. That's why enzyme inhibition studies are an important issue for drug design and biochemical applications. In this study, in vitro inhibition effect of some antipsychotic drugs on the purified carbonic anhydrase (CA) I and II isoenzymes were investigated by using CO2 as a substrate. CA I and II were purified from human erythrocytes by a simple one step procedure using Sepharose 4B-L-tyrosine-sulfonamide affinity column. The results showed that all the drugs inhibited the cytosolic carbonic anhydrases enzyme activity in a concentration-dependent fashion. Among the studied drugs, aripiprazole and pramipexole were found to be the most active one for hCA I (IC50: 3.64 and 5.37 mu M) and hCA II (IC50: 4.16 and 4.81 mu M) activity, respectively.Öğe Comparison of serum paraoxonase 1 (PON1) activities among different sheep breeds in Turkey(2011) Arslan, Mikail; Erzengin, Mahmut; Demir, DuduParaoxonase 1 (PON1, EC 3.1.8.1) is a calcium dependent mammalian enzyme that is synthesized primarily in the liver and is secreted into the serum where it is associated with High Density Lipoproteins (HDLs) and has a protective effect against oxidation of Low Density Lipoproteins (HDLs). Beside antioxidant and antiatherogenic properties, PON1 is also a detoxifier that can hydrolyze toxic organophosphates. Several studies have shown that PON1 can bind reversibly to organophosphate substrates which it hydrolyzes. Therefore, PON1 is the main means of protection of the nervous system against the neurotoxicity of organophosphates entering the circulation. This study was conducted to characterization of serum Paraoxonase 1 (PON1) activity from different sheep breeds namely Karacabey Merino, Kivircik, Tahirova, Akkaraman and Daglic. KM and Vmax values of five different sheep breed were determined by Lineweaver-Burk method. The values of Vmax/KM showed that Kivircik breed has the greatest PON1 activity, on the other hand, Karacabey Merino breed showed the least activity toward paraoxon substrate. © Medwell Journals, 2011.Öğe In vitro effects of some antibıotics on enzyme activity of carbonic anhydrase from bovine erythrocytes(Parlar Scientific Publications, 2011) Arslan, Mikail; Beyaztaş, Serap; Erzengin, MahmutThe purpose of this study was to investigate the in vitro effects of six commonly used veterinary medical drugs (Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM), Lypectin (TM) and Penoksal-la (TM)) on erythrocyte bovine carbonic anhydrase (CA, EC 4.2.1.1) activity. The enzyme was purified by affinity chromatography, and the purity was confirmed by SDS-PAGE. Inhibition or activation effects of six different antibiotics on the purified enzyme were determined using the CO2-hydratase activity method. IC50 values of the drugs that caused inhibition were determined by means of Activity % vs [Inhibitor] diagrams. The concentrations of Killoxacin (TM), Gentavet (TM), Geosol (TM), Primoxal (TM) and Lypectin (TM) that inhibited 50% of the enzymatic activity were 9.25, 3.79, 0.72, 6.59 and 2.12 mM, respectively. On the other hand, the enzyme activity was increased by Penoksal-la (TM).Öğe In vitro inhibition effect of some dihydroxy coumarin compounds on purified human serum paraoxonase 1 (PON1)(Humana Press, 2012) Erzengin, Mahmut; Başaran, İsmet; Çakır, Umit; Aybey, Aynur; Sinan, SelmaHuman serum paraoxonase 1 (PON1; EC 3.1.8.1) is a high-density lipoprotein associated, calcium-dependent enzyme that hydrolyses aromatic esters, organophosphates and lactones and can protect the low-density lipoprotein against oxidation. In this study, in vitro inhibition effect of some dihydroxy coumarin compounds namely 6,7-dihydroxy-3-(2-methylphenyl)-2H-chromen-2-one (A), 6,7-dihydroxy-3-(3-methylphenyl)-2H-chromen-2-one (B) and 6,7-dihydroxy-3-(4-methylphenyl)-2H-chromen-2-one (C) on purified PON1 were investigated by using paraoxon as a substrate. PON1 was purified using two-step procedures, namely ammonium sulphate precipitation and Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. The purified enzyme had a specific activity of 11.76 U/mg. The dihydroxy coumarin derivatives of A and B compounds inhibited PON1 enzyme activity in a noncompetitive inhibition manner with K (i) of 0.0080 +/- 0.256 and 0.0003 +/- 0.018 mM values, respectively. C compound exerted an uncompetitive inhibition of PON1 enzyme activity with K (i) of 0.0010 +/- 0.173 mM. Moreover, dihydroxy coumarin derivatives of A, B and C compounds were effective inhibitors on purified human serum PON1 activity with IC50 of 0.012, 0.022 and 0.003 mM values, respectively. IC50 value of unsubstituted 6,7 dihydroxy coumarin was found as 0.178 mM. The present study has demonstrated that PON1 activity is very highly sensitive to studied coumarin derivatives.Öğe Interactions of bovine serum albumin with humic acid-Cu(II) aggregates in poly(hydroxyethylmethacrylate) cryogel column(Elsevier, 2016) Sargın, İdris; Arslan, Gülsin; Erzengin, MahmutUnderstanding the interactions of proteins with humic acid in different media is essential for many applications. This paper investigates the interactions of bovine serum albumin with humic acid-Cu(II) complexes in a poly(hydroxyethylmethacrylate) cryogel column. Humic aggregates were immobilized within the cryogenic matrix. Protein sorption studies were conducted at different temperature, ionic strength, protein concentration, pH and flow-rate. Protein adsorption increased at lower pHs (close to the isoelectric point of the protein; 4.7) and higher temperatures but decreased at higher ionic strength and flow-rates. Both electrostatic and hydrophobic interactions played roles in the sorptive behavior of the protein molecules. Isotherm analysis showed the monolayer protein adsorption onto humic molecules immobilized column. (c) 2016 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.Öğe Investigation of protein adsorption performance of Ni2+-attached diatomite particles embedded in composite monolithic cryogels(Wiley Periodicals, 2011) Ünlü, Nuri; Ceylan, Şeyda; Erzengin, Mahmut; Odabaşı, MehmetAs a low-cost natural adsorbent, diatomite (DA) (2 mu m) has several advantages including high surface area, chemical reactivity, hydrophilicity and lack of toxicity. In this study, the protein adsorption performance of supermacroporous composite cryogels embedded with Ni2+-attached DA particles (Ni2+-ADAPs) was investigated. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic composite cryogel column embedded with Ni2+-ADAPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as crosslinker directly in a plastic syringe for affinity purification of human serum albumin (H SA) both from aqueous solutions and human serum. The chemical composition and surface area of DA was determined by XRF and BET method, respectively. The characterization of composite cryogel was investigated by SEM. The effect of pH, and embedded Ni2+-ADAPs amount, initial HSA concentration, temperature and flow rate on adsorption were studied. The maximum amount of HSA adsorption from aqueous solution at pH 8.0 phosphate buffer was very high (485.15 mg/g DA). It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Ni2+-ADAPs in poly(2-hydroxyethyl methacrylate) composite cryogel without significant loss of adsorption capacity. The efficiency of albumin adsorption from human serum before and after albumin adsorption was also investigated with SDS-PAGE analyses.Öğe Monolithic hydrophobic cryogel columns for protein separation(Springer, 2022) Erzengin, Mahmut; Baydemir Peşint, Gözde; Zenger, Okan; Odabaşı, MehmetThe present study was conducted for the synthesis of a novel supermacroporous monolithic hydrophobic adsorbent for lysozyme (Lyz) selected as a model protein from aqueous solution. After preparation of poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(l)-tyrosine methyl ester) monolithic cryogel column, 1-naphthylamine was covalently attached, and the prepared column was abbreviated as NA-Mcc. Scanning electron microscopy, Fourier transform infrared spectroscopy and Brunauer, Emmett and Teller device were utilized for the morphology, functional groups and surface area measurements of the column. Effects of several parameters including Lyz content of the adsorption solution, pH of the medium used, ambient temperature, type of salt and flow rate on the adsorption capacity of the polymeric material were examined in continuous operation. The maximum value achieved for Lyz adsorption from aqueous phase was found to be 105.8 mg/g in phosphate buffer. In addition, NA-Mcc was investigated in terms of reusability, and it was demonstrated that there is no significant change in the adsorption properties of prepared monolithic hydrophobic cryogels after 30 adsorption-desorption cycles.Öğe Purification and characterization of paraoxonase 1 (PON1) from swiss black, holstein, and montofon bovines(Humana Press, 2014) Erzengin, Mahmut; Demir, Dudu; Arslan, Mikail; Sinan, SelmaParaoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals and other vertebrates as well as in invertebrates. PONs exhibit a range of important activities, including drug metabolism and detoxification of organophosphates such as nerve agents. This study reports, for the first time, purification and biochemical characterization of serum PON1 from different bovine breeds namely Swiss Black, Holstein, and Montofon. Bovine serum PON1s were purified using ammonium sulfate precipitation followed by Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. SDS-polyacrylamide gel electrophoresis of the purified enzymes indicates a single band with an apparent MW of 43 kDa. The purified enzymes had a specific activity of 10.78, 27.00, and 22.38 U/mg for Swiss Black, Holstein, and Montofon bovines, respectively. The overall purification rates of our method were 262.47-, 2,476.90-, and 538.06-fold for Swiss Black, Holstein, and Montofon bovines, respectively. Furthermore, using phenyl acetate as a substrate, we determined the K (M) and V (max) values of the purified enzymes, as 0.80 mM, 1428.5 U/ml for Swiss Black; 0.40 mM, 714.3 U/ml for Holstein; and 0.50 mM, 1,111.1 U/ml for Montofon bovine. The present study has revealed that there is no substantial difference in PON1 activities among the studied bovine breeds.Öğe Purification of holstein bull semen paraoxonase 1 (pon1) by hydrophobic interaction chromatography and ınvestigation of ıts ınhibition kinetics by heavy metals(Humana Press, 2014) Dedeoğlu, Nurcan; Arslan, Mikail; Erzengin, MahmutIn this study, paraoxonase 1 (PON1; EC 3.1.8.1) was purified from bull semen, and some characteristics of the enzyme were investigated. In vitro inhibition effect of some heavy metals, including Cu2+, Mn2+, Cd2+, Zn2+, Ni2+, and Pb2+, on the activity of the purified enzyme was also investigated. The purification of bull semen PON1 procedure was composed of two steps: ammonium sulfate precipitation and Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. The enzyme, having a specific activity of 288 EU/mg proteins, was purified 22.67-fold with a yield of 89 %. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the purified enzyme showed the presence of a single band with an apparent MW of 66 kDa. The V (max) and K (M) values for the paraoxon substrate were determined as 100 EU and 8.0 x 10(-5) M, respectively. The inhibitory effects of different heavy metals on PON1 activity were determined by using the paraoxon as a substrate. The results showed that all the metals, except for Cd2+, inhibited the PON1 enzyme activity in a concentration-dependent fashion. IC50 values of Cu2+, Mn2+, Zn2+, Ni2+, and Pb2+ were found as 2.59 x 10(-3), 1.17 x 10(-3), 42.74 x 10(-3), 99.10 x 10(-3), 48.80 x 10(-3) mM, respectively. Conversely, Cd2+ increased the bull semen PON1 enzyme activity. The present study has demonstrated that Cu2+, Mn2+, Zn2+, Ni2+, and Pb2+ are serious toxic metals, which are able to increase the risk of oxidative stress development and a subsequent decrease of semen quality.Öğe Purification of polyphenol oxidase from Nevsehir potato (Solanum tuberosum L.) and investigation of its in vitro inhibition by some pesticides(American Chemical Society, 2014) Erzengin, Mahmut; Tozak, Kabil Özcan[Abstract Not Available]Öğe Sorption of DNA by diatomite-Zn (Ii) embedded supermacroporous monolithic p(HEMA) cryogels(2013) Özcan Tozak, Kabil; Erzengin, Mahmut; Sargın, İdris; Ünlü, NuriIn this study, the DNA sorption performance of diatomite-Zn(II) embedded supermacroporous monolithic p(HEMA) cryogels were investigated for the purpose of designing a novel adsorbent that can be utilized for DNA purification, separation and immunoadsorption studies such as removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma. Poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based monolithic cryogel column embedded with Zn2+-diatomite particles was prepared by free radical cryocopolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm). The polymerization reaction was initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After thawing, the monolithic composite cryogels were used for affinity sorption and then subsequent desorption of DNA molecules from aqueous solutions. Diatomite (DA) particles were characterized by XRF and BET method. The characterization of composite cryogel was done through SEM imaging. The effects of pH of the solution, initial DNA concentration, ionic strength, temperature and flow rates on adsorption were investigated to determine the optimum conditions for adsorption/desorption experiments. The particle embedding procedure was shown to yield significantly enhanced adsorption of DNA on the adsorbent. Furthermore, considering its excellent bio-compatibility, p(HEMA) cryogels are promising a candidate for further DNA sorption studies.Öğe Synthesis, characterization and biological assessment of a novel hydrazone as potential anticancer agent and enzyme inhibitor(Elsevier B.V., 2020) Noma, Samir Abbas Ali; Erzengin, Mahmut; Tunç, Tuncay; Balcıoğlu, SevgiIn present work, a novel hydrazone compound, namely 1-(5-bromo-2,3-dimethoxybenzylidene)-2-(pyridine-2-yl) hydrazine (5Br2DM2PH), was synthesized by condensation reaction in acidic conditions. The structure of this compound was detailed elucidated by 1H NMR, FT-IR, UV/VIS and XRD methods. Antioxidant activity of the 5Br2DM2PH compound was investigated by scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The 5Br2DM2PH compound exhibited high scavenging activity compared with reference antioxidant. To determine in vitro cytotoxic effect of its, cell viability was tested using MTT assay on human breast (MCF-7) and colon (Caco-2) cancer cell lines. The results showed that the cytotoxic effect of the 5Br2DM2PH is more potent than cisplatin. The IC50 value for MCF-7 is 16.8 ?M while the value for Caco-2 is 11.8 ?M. In addition, the in vitro inhibitory property of the compound 5Br2DM2PH was carried out on human paraoxonase-1 (hPON-1) and the IC50 value was found as 13.8 ?g. Our ambitions for the novel 5Br2DM2PH compound will guide many future studies in the field of medicine, analytical and drug applications, as well as new organic synthesis.Öğe Synthesis, spectroscopic, crystal structure, biological activities and theoretical studies. of 2-[(2E)-2-(2-chloro-6-fluorobenzylidene) hydrazinyllpyridine(Elsevier Science Bv, 2017) Özçelik, Nefise Dilek; Tunç, Tuncay; Çelik, Raziye Çatak; Erzengin, Mahmut; Özışık, HacıWe report in this paper the synthesis, spectroscopic, crystal structure, biological activities and theoretical results of the title compound. The crystal structure was defined by the X-ray diffraction (XRD) method. In addition, this newly synthesized hydrazone derivative was also subjected to its possible antioxidant activity with free radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals using butylated hydroxytoluene (BHT) as standard antioxidant. The structural calculations were performed by the density functional theory using the B3LYP method with 6-311++G(2d,2p) basis set. The calculated values were compared with experimental results. (C) 2017 Elsevier B.V. All rights reserved.
