Yazar "Dikici, Emrah" seçeneğine göre listele
Listeleniyor 1 - 19 / 19
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes(Elsevier B.V., 2023) Acet, Ömür; Ali Noma, Samir Abbas; Önal Acet, Burcu; Dikici, Emrah; Osman, Bilgen; Odabaşı, MehmetIn this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 °C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.Öğe Alpha-Amylase immobilized composite cryogels: some studies on kinetic and adsorption factors(Springer, 2021) Acet, Ömür; İnanan, Tülden; Acet, Burcu Önal; Dikici, Emrah; Odabaşı, MehmetStability of enzymes is a significant factor for their industrial feasibility. alpha-Amylase is an important enzyme for some industries, i.e., textile, food, paper, and pharmaceutics. Pumice particles (PPa) are non-toxic, natural, and low-cost alternative adsorbents with high adsorption capacity. In this study, Cu2+ ions were attached to pumice particles (Cu2+-APPa). Then, Cu2+-APPa embedded composite cryogel was synthesized (Cu2+-APPaC) via polymerization of gel-forming agents at minus temperatures. Characterization studies of the Cu2+-APPaC cryogel column were performed by X-ray fluorescence spectrometry (XRF), scanning electron microscopy (SEM), and Brunauer, Emmett, Teller (BET) method. The experiments were carried out in a continuous column system. alpha-Amylase was adsorbed onto Cu2+-APPaC cryogel with maximum amount of 858.7 mg/g particles at pH 4.0. Effects of pH and temperature on the activity profiles of the free and the immobilized alpha-amylase were investigated, and results indicate that immobilization did not alter the optimum pH and temperature values. k(cat) value of the immobilized alpha-amylase is higher than that of the free alpha-amylase while K-M value increases by immobilization. Storage and operational stabilities of the free and the immobilized alpha-amylase were determined for 35 days and for 20 runs, respectively.Öğe ?-Amylase immobilized composite cryogels: Some studies on kinetic and adsorption factors(Springer, 2021) Acet, Ömür; İnanan, Tülden; Önal Acet, Burcu; Dikici, Emrah; Odabaşı, MehmetStability of enzymes is a significant factor for their industrial feasibility. ?-Amylase is an important enzyme for some industries, i.e., textile, food, paper, and pharmaceutics. Pumice particles (PPa) are non-toxic, natural, and low-cost alternative adsorbents with high adsorption capacity. In this study, Cu2+ ions were attached to pumice particles (Cu2+-APPa). Then, Cu2+-APPa embedded composite cryogel was synthesized (Cu2+-APPaC) via polymerization of gel-forming agents at minus temperatures. Characterization studies of the Cu2+-APPaC cryogel column were performed by X-ray fluorescence spectrometry (XRF), scanning electron microscopy (SEM), and Brunauer, Emmett, Teller (BET) method. The experiments were carried out in a continuous column system. ?-Amylase was adsorbed onto Cu2+-APPaC cryogel with maximum amount of 858.7 mg/g particles at pH 4.0. Effects of pH and temperature on the activity profiles of the free and the immobilized ?-amylase were investigated, and results indicate that immobilization did not alter the optimum pH and temperature values. kcat value of the immobilized ?-amylase is higher than that of the free ?-amylase while KM value increases by immobilization.Öğe Anticholinergic, antioxidant, and antibacterial properties of vitex agnus-castus L. seed extract: assessment of Its phenolic content by LC/MS/MS(John Wiley and Sons Inc, 2022) Kavaz, Arzu; Işık, Mesut; Dikici, Emrah; Yüksel, MehmetIn this current study, Vitex agnus-castus seed ethanol extracts were analyzed for their phytochemical component content, anticholinergic and antioxidant activities, and antibacterial properties. The phenolic compound composition of these seeds was determined by using LC/MS/MS. Antioxidant activity of the seeds was examined by the DPPH, ABTS, Fe3+–Fe2+ reducing, and CUPRAC. Also, the anticholinergic activity was measured by the inhibition of acetylcholinesterase (AChE). The antibacterial activity was performed by disc diffusion and minimum inhibitory concentration methods. The main phenolic compound was vanillic acid (22812.05 ?g/L) and followed by luteolin, fumaric acid, quercetin, caffeic acid, 4-hydroxybenzoic acid, salicylic acid, kaempferol, butein, ellagic acid, resveratrol, catechin hydrate, phloridzin dehydrate, naringenin, respectively. The DPPH free radical scavenging value of ethanol extract of plant seeds was 9.41 %, while the ABTS radical scavenging activity was determined as 12.66 %. The ethanol extract of the seeds exhibited antibacterial activity on Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium, differently. S. aureus was found to be more susceptible to the extract than other bacteria. Also, the inhibition effect of seed ethanolic extract on the AChE with IC50 values were 36.34±5.6 ?g/mL. From the results, V. agnus-castus seed can be suggested as a promising natural antioxidant and antibacterial candidate for the preservation of foods.Öğe Antioxidant activity and total phenolic/flavonoid contents of phlomis pungens L.(Hacettepe Üniversitesi, 2017) Işık, Mesut; Dikici, Emrah; Tohma, Hatice; Köksal, EkremPlants generate secondary metabolites, which could be used as novel therapeutic compounds. These compounds are well known for their beneficial effects on human health. Thus, it is important to evaluate total phenolic and flavonoid contents as well as antioxidant activities of different plants. The present study was conducted to determine the total phenolic/flavonoid content and antioxidant activity of ethanol extract prepared from leaves of Phlomis pungens L. The total phenolic content of the ethanol extract of the sample were determined using the Folin-Ciocalteu reagent. Antioxidant activity of Phlomis pungens L. was determined using different in vitro experimental models, which include DPPH, FRAP, CUPRAC and potassium thiocyanate method. Total phenolic and flavonoid contents of Phlomis pungens L. were found to be 68.4 mg GAE/g and 4.9 mg QE/g, respectively. Antioxidant activity of the sample was comparable to commercial antioxidant standards (BHT, ?- tocopherol and trolox). The results showed that Phlomis pungens L has moderate free radical scavenging and reducing capacity. Overall, this study discovers total phenolic and flavonoid content as well as antioxidant properties of Phlomis pungens LÖğe Chemical composition and nutrient profiles of nine red macroalgae species(BioMed Central Ltd, 2024) Yücetepe, Aysun; Kırkın, Celale; Ayar, Eda Nur; Soylukan, Caner; Dikici, Emrah; Özçelik, Beraat; Okudan, Emine ŞükranNine red macroalgae (Amphiroa rigida, Gracilaria bursa-pastoris, Gracilaria gracilis, Grateloupia torture, Jania rubens, Laurencia obtusa, Laurencia pyramidalis, Liagora viscida, and Pterocladiella capillaries) were collected from coastal waters of Türkiye, and their proximate, fatty acid, soluble carbohydrate, and mineral profiles were investigated in the present study. According to the results, the crude protein content of the samples was between 4% and 23.8%, and four of the samples (G. turuturu, L. obtusa, L. pyramidalis, and P. capillacea) contained more than 10% protein. The crude lipid content of all the samples was below 1.6%, and the total carbohydrate content was between 38.3% and 76.9%. The macroalgae samples were generally richer in saturated fatty acids, palmitic acid being the most abundant, whereas G. gracilis had the highest content of unsaturated fatty acids (55.8%). All samples exhibited high contents of myo-inositol or glucose. Also, the samples generally had a good composition of minerals. Still, the heavy metal (i.e., Pb and Cd) content of Gracilaria gracilis was higher (59.6 µg/kg, P < 0.05) than those of the other algae samples. This study provides valuable insight into the chemical composition and fatty acid, mineral, and soluble carbohydrate profiles of Amphiroa rigida, Gracilaria bursa-pastoris, Gracilaria gracilis, Grateloupia turuturu, Jania rubens, Laurencia obtusa, Laurencia pyramidalis, Liagora viscida, and Pterocladiella capillacea from Türkiye.Öğe Determination of Antioxidant Activity of The Leaves of Hawthorn (Crataegus Monogyna)(Iğdır Üniversitesi, 2023) Dikici, Emrah; Köksal, EkremThis study was carried out to make decision the antioxidant activity of the leaves of hawthorn (Crataegus monogyna), which is a natural source of antioxidants. For this purpose, the leaves of hawthorn were collected and dried, and an ethanol extract was prepared. Total antioxidant activity (with respect to the ferric thiocyanate method), ion reduction capacity of Cu2+ (copper method), and Fe3+ (potassium ferricyanide reduction method) were determined on the prepared ethanol extracts. Additionally, scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) and determination of the total phenolic-flavonoid compound content were carry out. The extracts obtained from Hawthorn (Crataegus monogyna) leaves were found to inhibit lipid peroxidation (of linoleic acid emulsion) by 40.9%. This inhibition occurred at a concentration of 10 µg/mL of the extracts. At the similar concentration, BHT exhibited 68.3% inhibition, while trolox exhibited 29.9% inhibition. Hawthorn (Crataegus monogyna), which was found to have a higher total phenolic content than trolox, exhibited significant DPPH scavenging and metal reduction activities. During the investigation of the antioxidant activity of the ethanol extract obtained from Crataegus monogyna, ?-tocopherol, its water-soluble analogue trolox and BHT (butylated hydroxytoluene) were utilized as reference antioxidant compounds.Öğe Determination of secondary metabolites of Cydonia oblonga (Quince) by LC-MS/MS method together with evaluation of its antioxidant and cholinergic potentials(ACG Publications, 2024) Dikici, Emrah; Altın, Sevgi; Alp, Cemalettin; Işık, Mesut; Köksal, Ekrem; Gülçin, İlhamiIn this study, quantification of secondary metabolites of Cydonia oblonga (Quince) leaves by LC-MS/MS and antioxidant, cholinergic and ?-Glycosidase activities were reported. LC-MS/MS analyses of the studied extract showed that the highest phenolic constituents of species were catechin (2024.9 ± 80.9 ?g/L), vanillic acid (1121.8 ± 39.3 ?g/L) and fumaric acid (486.7 ± 16.5 ?g/L). The ethanol extract of quince leaves inhibited the ?-glycosidase enzyme with an IC50: 0.6 ?g/mL value. In order to determine the anti-Alzheimer activity of the plant extract, inhibition studies were carried out on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. The IC50 values of the plant extract were found to be 1.3 ?g/mL for AChE and 1.5 ?g/mL for BChE. In addition, free radical scavenging and metal reduction tests were performed to determine the antioxidant activity of the plant and the results were compared with Trolox. It was understood that the antioxidant potential of the plant was lower than Trolox. The results from biological abilities, molecular docking of catechin hydrate as abundant phenolic compounds in quince leaves extract to be an inhibitor candidate for AChE, BChE and ?-glycosidase.Öğe Evaluation of biological (feed, water), seasonal, and geological factors affecting the heavy metal content of raw milk(Academic Press Inc., 2023) Özbay, Sena; Dikici, Emrah; Soylukan, CanerIn volcanic regions worldwide, soil and water can be contaminated with heavy metals. Aksaray province is also an important region in this respect. Because this city is located around Hasandağı, an extinct volcanic mountain. In addition, the city is also an important dairy center. In this regard, evaluating the milk produced in Aksaray in terms of heavy metal contamination and studying the effect of feed and water on this contamination is essential. For this purpose, feed, water, and milk samples from 7 milk producers determined in Aksaray province were collected in 3 different seasons within six months. The presence and levels of arsenic (As), aluminum (Al), nickel (Ni), cadmium (Cd), and lead (Pb) were determined by ICP-MS analysis of the 126 samples obtained. The study found varying levels of heavy metals in feed, water, and milk. It was also observed that milk contained considerably lower quantities of metals and metalloids than feed and water. In addition, it has been determined that the distance to Hasandağı does not affect the trace element levels in water, milk, and feed, but there is a significant relationship between seasonal changes and trace elements in the samples. According to the analysis results, the highest level is the feed's aluminum level (298,290.1 µg/kg). However, the aluminum level in milk remained well below this level (96.15 µg/kg). It was determined that the cadmium level in milk reached the highest level in spring (average 0.06 µg/kg), whereas lead levels reached the highest level in summer (average 2.14 µg/kg). On the other hand, the arsenic level showed a relatively small change according to the distance to the volcanic region. The average arsenic level in milk from regions near the volcanic area was measured as 1.01 µg/kg. In remote areas, this amount was measured as 0.94 µg/kg, Furthermore, the highest heavy metal level in the milk was 182.08 µg/kg for nickel during the summer months in the study.Öğe Inhibition of bacterial adhesion by epigallocatechin gallate attached polymeric membranes(Elsevier, 2023) Acet, Ömür; Dikici, Emrah; Önal Acet, Burcu; Odabaşı, Mehmet; Mijakovic, Ivan; Pandit, SantoshMicrobial adhesion and formation of biofilms cause a serious problem in several areas including but not limited to food spoilage, industrial corrosion and nosocomial infections. These microbial biofilms pose a serious threat to human health since microbial communities in the biofilm matrix are protected with exopolymeric substances and difficult to eradicate with antibiotics. Hence, the prevention of microbial adhesion followed by biofilm formation is one of the promising strategies to prevent these consequences. The attachment of antimicrobial agents, coatings of nanomaterials and synthesis of hybrid materials are widely used approach to develop surfaces having potential to hinder bacterial adhesion and biofilm formation. In this study, epigallocatechin gallate (EGCG) is attached on p(HEMA-co-GMA) membranes to prevent the bacterial colonization. The attachment of EGCG to membranes was proved by Fourier-transform infrared spectroscopy (FT-IR). The synthesized membrane showed porous structure (SEM), and desirable swelling degree, which are ideal when it comes to the application in biotechnology and biomedicine. Furthermore, EGCG attached membrane showed significant potential to prevent the microbial colonization on the surface. The obtained results suggest that EGCG attached polymer could be used as an alternative approach to prevent the microbial colonization on the biomedical surfaces, food processing equipment as well as development of microbial resistant food packaging systems.Öğe “Lab-on-pol” colormatic sensor platforms: Melamine detection with color change on melamine imprinted membranes(Elsevier Inc., 2023) Dikici, Emrah; Önal Acet, Burcu; Acet, Ömür; Odabaşı, MehmetThere is an urgent need to improve a specific, equipment-free and user friendly technique for detecting melamine in food samples. Here, melamine imprinted polymeric membranes were synthesized, and tested for selectivity in aqueous solutions against cyromazine used as competitor agent, and real milk samples spiked with melamine. Scanning electron microscope, atomic force microscopy, energy-dispersive X-ray and optical profilometer devices were used for characterization. Fe+3 ions were used as marker to obtain color change based on melamine concentration. The color intensity on MIP membranes was monitored by means of a color analysis application (Image J software) via a smartphone. Some obtained results as fallows: Melamine binding to 3D cavities of membrane came true in about 20 min. The linear regression plot showed a correlation coefficient (R2) of 0.995 in the range of 10 ?M-50 ?M according to Beer Lambert's law. A low concentration of melamine, (e.g., 10 µM) was determined in raw milk. Melamine imprinted polymeric membranes showed 7.575 times more sensitivity for melamine than cyromazine. Limit of detection (LOD) and limit of quantification (LOQ) were computed as 9.9 µM and 30.2 µM, respectively. Here, a new kind of smart polymer was designed, and successfully applied for the easy, portable and on-site colorimetric analysis via imprinted polymers, and named as “lab-on-pol”system. HPLC analysis method was also used to validate this new analysis method.Öğe Peptit temelli nanomateryallerin hazırlanması ve hücresel etkileşimlerinin incelenmesi(Aksaray Üniversitesi Fen Bilimleri Enstitüsü, 2024) Dikici, Emrah; Odabaşı, MehmetKanser hastalıklarına karşı alışılagelmiş tedavi yöntemlerinde rastlanan tümör invazyonu, farklı dokulara tümörün yayılması ve çoklu ilaç direnci gibi dezavantajlardan ötürü, yeni yöntemler geliştirmek büyük öneme sahiptir. Gen terapisi alanında terapötik geni hedef dokuya taşıyan, kendiliğinden düzenlenme, biyo-uyumluluk ve biyo-bozunurluk gibi avantajlara sahip nano-materyallerin üretiminde öne çıkan peptit temelli yapılara, moleküler tanıma fonksiyonları nedeniyle her geçen gün ilgi artmaktadır. Tez kapsamında, gen susturucu olarak kullanılan siRNA'ları kanser hücrelerine taşımak üzere kendiliğinden düzenlenebilen boyutu ve dengelenmiş yüzey yükü bakımından yeni olan difenilalanin amid (FFA) temelli nano-materyaller (NM) sentezlendi. FFA-NM'ler sentezlendikten sonra Ca2+ iyonları ile dekore edildi. Karakterizasyon çalışmaları kapsamında SEM (taramalı elektron mikroskobu), TEM (transmisyon elektron mikroskobu), MS (kütle spektrometresi), DLS (dinamik ışık saçılma), Zeta-potansiyel ölçümü, Image J boyut değerlendirmesi ve FTIR (Fourier dönüşümü kızılötesi) spektroskopisi ile analiz edildi. Biyolojik moleküllerle korona oluşturma potansiyellerini değerlendirmek için sentezlenen FFA ve Ca2+-FFA-NM'ler HSA, IgG ve DNA molekülleri ile etkileştirildi. NM'lerin yüzeyinde oluşan korona tabakası DLS ve spektrofotometrik olarak değerlendirildi. Gerçekleştirilen hücre toksisite ve internalizasyon testlerinden sonra elde edilen bazı bulgular şöyle özetlenebilir: Ortalama boy dağılımı 87,93 nm olan FFA-NM'lerin yüzey yükleri -20,1 mV iken Ca2+ dekorasyonundan sonra +9,3 mV olmuştur. Yüzey yük dağılımı, Ca2+-FFA-NM'lerin HSA, IgG ve DNA molekülleri ile oluşturduğu korona yapılarına göre de sırasıyla -7,6, -3,7 ve -9,7 mV olarak belirlenmiştir. Ca2+-FFA-NM'lerin HeLa ve HT29 hücre hatlarıyla 24 saatlik etkileşimleri sonrasında toksisite görülmemiştir. Farklı siRNA türlerinin (Mcl-1 ve Bcl-xL) Ca2+-FFA-NM'ler ile oluşturduğu komplekslerinin HeLa ve HT29 hücre hatlarına internalize olduğu ve canlılıklarında yaklaşık %36 oranında bir azalmaya neden olduğu görülmüştür. Bu çalışmada kullanılan FFA tabanlı NM'ler biyo-uyumlu olup, susturucu siRNA'ların kanser hücrelerine internalizasyon derecesini artırmak için yapılacak olan daha ileri çalışmalarda umut vadedeceği düşünülmektedir.Öğe Phenolic Content Analysis of Two Species Belonging to the Lamiaceae Family: Antioxidant, Anticholinergic, and Antibacterial Activities(Multidisciplinary Digital Publishing Institute (MDPI), 2024) Ndhlala, Ashwell R.; Işık, Mesut; Kavaz Yüksel, Arzu; Dikici, EmrahThe Lamiaceae family are utilized as ornamental, medicinal, and food supplements throughout the world. The current study focuses on a comparative analysis of the phenolic compositions and bioactivities (including antioxidant, anticholinergic, and antibacterial activities) of ethanolic extracts derived from the aerial parts of the two species (Lavandula stoechas L. and Thymus sipyleus Boiss). The presence of phenolic compounds and phytochemicals in the plant extracts was identified using the LC-MS/MS technique. The LC-MS/MS analysis revealed that vanillic acid (125,596.66 µg/L) was the most abundant phytochemical in L. stoechas. Kaempferol (8550.52 µg/L) was the most abundant substance in Thymus sipyleus. The assessment of the antioxidant efficacy of the species extracts was conducted using the DPPH (2.2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2.2?-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), Fe3+–Fe2+ reducing, and CUPRAC (Cu2+–Cu+ reducing) assays. The anticholinergic activity of the samples was determined using the acetylcholinesterase (AChE) inhibition assay. The results of antioxidant activity were higher in the T. sipyleus than in the L. stoechas ethanol extracts. The extracts of L. stoechas exhibited radical scavenging activity ranging from 15 to 18%, while T. sipyleus had activity effects ranging from 34% to 38%. The AChE inhibition potential for L. stoechas and T. sipyleus extracts as IC50 values were 0.221 ± 0.01 mg/mL and 0.067 ± 0.02 mg/mL, respectively. The antibacterial effects of the ethanolic extracts of these species against pathogenic bacteria isolates were determined using the MIC (minimal inhibitory concentration) method. These findings indicated that the extracts from L. stoechas and T. sipyleus possess the potential to be natural antioxidants in the realm of food preservation. Additionally, their antioxidant, anticholinergic, and antimicrobial properties suggest potential therapeutic utility in the management of certain diseases.Öğe Phenolic profile and antioxidant, anticholinergic, and antibacterial properties of corn tassel(MDPI, 2022) Al-Khayri, Jameel M.; Yüksel, Arzu Kavaz; Yüksel, Mehmet; Işık, Mesut; Dikici, EmrahCorn tassel (CT) is a waste part of the corn plant. It is a good co-product and rich in terms of bioactive compounds and phytochemicals. This research tried to show the phenolic profile, antioxidants, anticholinergic activities, and antibacterial properties of CT ethanol extract. The phenolic content analysis of the CT was determined quantitatively by LC-MS/MS, and the antioxidant capacity was measured using ABTS, DPPH, Cu2+-Cu+, and Fe3+-Fe2+ reducing methods. The anticholinergic measurements of CT were detected by inhibition of acetylcholinesterase (AChE). The antibacterial activity was determined by MIC and disc diffusion methods. Many phenolic compounds such as vanillic acid, caffeic acid, fumaric acid, acetohydroxamic acid, butein, myricetin, resveratrol, catechin hydrate, and 4-hydroxybenzoic acid were detected in ethanol extract of CT. The obtained plant ethanol extract had a 7.04% DPPH value, while it showed ABTS activity at 9.45%. Moreover, it had a 0.10 mg/mL inhibition effect on the AChE in terms of IC50 values. The ethanol extract of the CT had an antibacterial property on the investigated bacteria at different ratios. In conclusion, this research aims to consider CT as a source of phenolic compounds and to reveal its bioactive properties and its effects on the treatment of some diseases.Öğe Phytochemical, phenolic profile, antioxidant, anticholinergic and antibacterial properties of Epilobium angustifolium (Onagraceae)(Springer, 2021) Yüksel, Arzu Kavaz; Dikici, Emrah; Yüksel, Mehmet; Işık, Mesut; Tozoğlu, Fatih; Köksal, EkremEpilobium angustifolium is widely used in medicine for disease treatments, as well as in the cosmetic and food industries. The aim of this research was to investigate the antioxidant, and anticholinergic properties, phenolics profile and antibacterial activities of the E. angustifolium ethanol extract. The analysis of phenolic compounds was performed with LC-MS/MS. The antioxidant capacity (radical scavenging, metal-reducing power and total antioxidant activity) was assessed by DPPH, ABTS, Cu2+-Cu+ reducing (CUPRAC), Fe3+-Fe2+ reducing and ferric thiocyanate methods. The antibacterial activity was determined by disc diffusion and MIC (Minimum inhibitory concentration) methods and the anticholinergic property was predicted by inhibition of acetylcholinesterase (AChE). The major phenolic compounds, founding in the plant extract were luteolin, fumaric acid, vanillic acid, and caffeic acid. The ethanol extract of the plant showed DPPH free radical scavenging value of 11.3%, while the ABTS radical scavenging activity was 19.4% and showed moderately metal-reducing power. Also, the extract had 39.3% inhibition on lipid peroxidation of linoleic acid emulsion and showed an inhibition effect on the AChE with IC50 values (0.14 mg mL(-1)). The ethanol extract of the plant showed antibacterial effect on Staphylococcus aureus, Escherichia coli, and Salmonella Typhimurium at different levels. These results suggested that E. angustifolium extract might be a suitable natural antioxidant in the preservation of foods by preventing the oxidation of polyÖğe Pumice particle interface: a case study for immunoglobulin G purification(Springer Science and Business Media Deutschland GmbH, 2020) Alacabey, ,İhsan; Acet, Ömür; Önal, Burcu; Dikici, Emrah; Karakoç, Veyis; Gürbüz, Fatma; Alkan, Hüseyin; Odabaşı, MehmetCryogels with embedded natural adsorbent are new trend of chromatographic media for separation of biomolecules. In this report, experimental determination of immunoglobulin G (IgG) purification by Cu2+-attached pumice particles unified cryogel (Cu2+-PPUC) was performed. For this purpose, after preparation of Cu2+-attached pumice particles, they were unified with 2-hydroxyethyl methacrylate monomers to produce Cu2+-PPUC through polymerization of gel-forming precursors at subzero temperatures. IgG separation experiments were accomplished in a continuous column system. The highest binding capacity (596.8 mg/g) was obtained by working with 0.02 M phosphate buffer at pH 6.0. The chemical analysis of pumice was examined by X-ray fluorescence spectrometer. Scanning electron microscopy was performed to identify the morphology of Cu2+-PPUC. Langmuir adsorption model was best fitted to interaction when compared to Freundlich model. Temkin model was utilized to characterize adsorption, energetically. Purification ability of Cu2+-PPUC for IgG was shown with high selectivity via reducing SDS–PAGE electrophoresis.Öğe Self-Assembled Short Peptide Nanostructures: ‘’Dipeptides’’(Kırgızistan-Türkiye Manas Üniversitesi, 2023) Dikici, Emrah; Önal Acet, Burcu; Gök, Tuba; Acet, Ömür; Odabaşı, MehmetDipeptides are short peptide molecules formed by the peptide bond between two amino acids, and they play significant roles in various biological processes (such as protein synthesis, nutrient absorption, cellular signaling, immune response). Short peptides have a prominent place in the design of self-assembling materials. In particular, dipeptides have gained considerable attention in the field of biotechnology as a type of self-organizing nanostructure due to their low cost, simplicity of synthesis, biocompatibility, and tunability of functionality. However, there is limited knowledge about peptide and protein-based nanostructures in the literature. Therefore, more information is needed on dipeptide nanostructures, especially in terms of their potential applications for biomedical purposes. This review focuses on dipeptide nanostructures, particularly their potential uses in biomedical applications, and provides a broader perspective on the advantages, challenges, synthesis, interactions, and applications of these nanostructures.Öğe Synthesis, Characterization, and Investigation of Corona Formation of Dipeptide-Based Nanomaterials(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Dikici, Emrah; Önal Acet, Burcu; Bozdoğan, Betül; Acet, Ömür; Halets-Bui, Inessa; Shcharbin, Dzmitry; Odabaşı, MehmetPeptide-based nanomaterials can be easily functionalized due to their functional groups, as well as being biocompatible, stable under physiological conditions, and nontoxic. Here, diphenylalanineamide-based nanomaterials (FFANMs) were synthesized, decorated with Ca2+ ions to set the surface charge, and characterized for possible use in gene delivery and drug release studies. FFANMs were characterized by SEM, TEM, dynamic light scattering (DLS), and LC-MS/MS. Corona formation and biocompatible studies were also carried out. Some of the data obtained are as follows: FFANMs have a diameter of approximately 87.93 nm. While the zeta potentials of FFANMs and Ca2+@FFANMs were −20.1 mV and +9.3 mV, respectively, after corona formation with HSA and IgG proteins, they were shifted to −7.6 mV and −3.7 mV, respectively. For gene delivery studies, zeta potentials of Ca2+@FFANMs and DNA interactions were also studied and found to shift to −9.7 mV. Cytotoxicity and biocompatibility studies of NMs were also studied on HeLa and HT29 cell lines, and decreases of about 5% and 10% in viability at the end of 24 h and 72 h incubation times were found. We think that the results obtained from this study will assist the groups working in the relevant field. © 2024 by the authors.Öğe Things to know and latest trends in the design and application of nanoplatforms in cancer treatment(Springer, 2024) Yıldırım, Metin; Acet, Burcu Önal; Dikici, Emrah; Odabaşı, Mehmet; Acet, ÖmürNanotechnology has a crucial potential in cancer treatment. Nowadays, the trend of materials science has also contributed to the development of new nanoscale carrier systems, especially for use in the therapy of cancer patients. Many nanocarriers have been developed and continue to be developed for cancer therapy. Nanocarrier-based systems are widely used in cancer imaging, diagnostics, and therapeutics due to its promising properties and potential to increase therapeutic efficacy. The design and use of nano-based delivery systems for cancer therapy are very promising for the future of cancer therapy. This review article has been presented to the literature in order to display the prominent trends in cancer treatment in recent years. For this purpose, key points in designing an ideal nanoplatforms, the latest important nanocarriers such as liposomes, dendrimers, DNA origami, metallic nanoparticles, responsive, smart, biodegradable and biocompatible nanocarriers, together with recent studies, future perspectives are presented. Graphical Abstract: (Figure presented.).
