Tokat ili çevre köylerinden elde edilen sütlerde Brucella cinsi bakterilerin varlıgının araştırılması
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Aksaray Üniversitesi
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Brucelloz, Brucella cinsi bakterilerin neden olduğu en sık görülen zoonotik hastalıklardan biridir. Hastalık sığır, koyun, keçi, köpek ve domuz gibi memeli hayvanların dişilerinde genital organlanna yerleşerek yavru atma, infertilite, mastitis, erken doğum, plasenta retensiyonu ve metritise; erkeklerde ise orşitise (erbezi iltihabı) neden olur. Bu çalışmada, Tokat ili ve ilçelerine ait köylerden toplanan sütlerde Brucelloz etmeninin prevalansının belirlenmesi amaçlanmıştır. Bu amaçla 161 inek, 58 koyun ve 33 keçi sütü örneklerinde Brucelloz etmeni Milk Ring Test (MRT) ve direkt ekim yöntemi ile araştırılmıştır. Çalışma sonucunda 161 inek sütünden 24'ü, 58 koyun sütünden 20'si ve 33 keçi sütünden 5'inin MRT sonucu pozitif bulunmuştur. Toplamda 252 adet çiğ süt örneğinden 49 adedinin, yani % 19.41'inin MRT sonucu pozitif bulunmuştur. MRT'si pozitif olan 49 örnekten yapılan kültür sonrası 2 adet süt örneğinden Brucella melitensis biyotip 3 izole edilebilmiştir. Brucella izole edilen 2 süt örneğinin de aynı sürüden 2 farklı koyuna ait olduğu belirlenmiştir. Böylece tüm çalışılan örneklerin % 0.79'unda, MRT pozitif çıkan örneklerin % 4.08'inde ve koyun sütü örneklerinin % 3.45'inde Brucella etkeni izole edilmiştir. Tokat ili ve ilçelerine ait köy ve kasabalardan toplanan sütlerle yapılmış ilk çalışma özelliğinde olan araştırmamız sonucunda örneklerin % 19.41 'inde MRT sonucu pozitif bulunmuş, % 0.79'unda ise etmen direkt ekim yöntemiyle izole edilmiştir. Böylece, bu çalışmayla Tokat ili ve ilçelerindeki hayvanlarda belirli oranda Brucella etmeninin var olduğu belirlenmiş ve süt ürünlerinin Brucelloz bakımından risk oluşturabileceğini göstermiştir.
Brucellosis is one of the most common zoonotic diseases caused by Brucella. The disease is located genital organs in the females of mammals such as cattle, sheep, goats, dogs and pigs and cause to infertility, mastitis, preterm birth, placenta retention and metritis; in men it causes orchitis (gonorrhea inflammation). The bacteria can also spread from animals to humans and cause disease in humans. The aim of this study was to determine the prevalence of Brucellosis in milk collected from villages of Tokat province and districts. For this purpose, 161 cows, 58 sheep and 33 goat milk samples were investigated by Brucellosis agent with Milk Ring Test (MRT) and direct sowing method. At the end of the study, 24 of 161 cow's milk, 20 of 58 sheep's milk and 5 of 33 goat's milk were found to be positive by MRT. Of the 252 raw milk samples, 49 (n = 19.41%) were positive for MRT. Brucella melitensis biotype 3 were isolated from the 2 milk samples after the culturing from 49 samples with positive MRT. It was determined that 2 milk samples isolated from Brucea belong to 2 different sheep from the same herd. Thus, Brucella was isolated in 0.79% of all studied samples, 4.08% of MRT positive samples and 3.45% of sheep milk samples. As a result of our first study with milk collected from the villages and towns of Tokat province and districts, 19.41% of the samples were positive for MRT and 0.79% of the samples were isolated by direct planting method. Thus, in this study, it was determined that there is a certain amount of Brucella agent in Tokat province and its districts and it shows that dairy products may pose a risk for Brucellosis.
Brucellosis is one of the most common zoonotic diseases caused by Brucella. The disease is located genital organs in the females of mammals such as cattle, sheep, goats, dogs and pigs and cause to infertility, mastitis, preterm birth, placenta retention and metritis; in men it causes orchitis (gonorrhea inflammation). The bacteria can also spread from animals to humans and cause disease in humans. The aim of this study was to determine the prevalence of Brucellosis in milk collected from villages of Tokat province and districts. For this purpose, 161 cows, 58 sheep and 33 goat milk samples were investigated by Brucellosis agent with Milk Ring Test (MRT) and direct sowing method. At the end of the study, 24 of 161 cow's milk, 20 of 58 sheep's milk and 5 of 33 goat's milk were found to be positive by MRT. Of the 252 raw milk samples, 49 (n = 19.41%) were positive for MRT. Brucella melitensis biotype 3 were isolated from the 2 milk samples after the culturing from 49 samples with positive MRT. It was determined that 2 milk samples isolated from Brucea belong to 2 different sheep from the same herd. Thus, Brucella was isolated in 0.79% of all studied samples, 4.08% of MRT positive samples and 3.45% of sheep milk samples. As a result of our first study with milk collected from the villages and towns of Tokat province and districts, 19.41% of the samples were positive for MRT and 0.79% of the samples were isolated by direct planting method. Thus, in this study, it was determined that there is a certain amount of Brucella agent in Tokat province and its districts and it shows that dairy products may pose a risk for Brucellosis.
Description
Keywords
Süt, MRT, Tokat, Brucella, Milk
Journal or Series
Aksaray Üniversitesi Tıp Bilimleri Dergisi / Aksaray University Journal of Medical Sciences
WoS Q Value
Scopus Q Value
Volume
1
Issue
1
