Fe3+ takılı nanoflower'ların sentezi ve DNA'ya karşı özgüllüğünün araştırılması
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Dosyalar
Tarih
2020
Yazarlar
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Yayıncı
Aksaray Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Saflaştırma yönteminin ve adsorban tipinin seçimi, deoksiribonükleik asit (DNA) gibi bir biyomolekülün saflaştırılması için yüksek öneme sahiptir. Nanoflower'lar yeni geliştirilmiş bir adsorban sınıfıdır. Bitki çiçeklerine yapısal olarak çok benzerlik gösterdikleri için, nano çiçekler olarak adlandırılırlar. Burada, bakır fosfat nanoflower'lar [(Cu3(PO4)2·3H2O), CP-NF'ler] sentezlendikten sonra, yüzeylerine Fe3+ iyonları takılmıştır. Elde edilen Fe3+-CP-NF'ların, DNA içn bazı adsorpsiyon parametrelerinin incelenmesinden önce, taramalı elektron mikroskopisi (SEM), enerji dağıtıcı X-ışını (EDX), X-ışını kırınımı (XRD) ve fourier dönüşümlü kızılötesi spektroskopisi (FTIR) karekterizsyonları yapılmıştır. Elde edilen adsorpsiyon deneylerinin sonuçlarından bazıları, aşağıdaki verilmiştir: Fe3+-CP-NF'ler üzerinde maksimum DNA adsorpsiyonu, pH 7 ve 25 °C'de 1.5 mg/mL DNA başlangıçkonsantrasyonunda 845.8 mg/g gibi mükemmel bir değer olarak bulunmuştur. Hangi adsorpsiyon modelinin uygun olduğunu belirlemek için Langmuir ve Freundlich adsorpsiyon denklemleri uygulanmış ve 0.9885'lik bir R2 ile Langmuir modelinin uyumlu olduğu görülmüştür.
Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribo nucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers. Herein, after synthesize ofcopper phosphate three hydrate nanoflowers [(Cu3(PO4)2.3H2O), CP-NFs], Fe3+ ions were attached to their surfaces. Obtained Fe3+-CP-NFs, before investigation of some adsorption parameters for DNA, they were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR). Some attained data from the results of adsorption experiments as follows: maximum DNA adsorption on Fe3+-CP-NFs was found as an excellent value like 845.8 mg/g with an initial concentration of 1.5 mg/mL DNA, at pH 7 and 25 °C. Langmuir and Freundlich adsorption equations were applied to determine which adsorption model was appropriate, and it was seen that Langmuir model was fit with a R2 of 0.9885.
Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribo nucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers. Herein, after synthesize ofcopper phosphate three hydrate nanoflowers [(Cu3(PO4)2.3H2O), CP-NFs], Fe3+ ions were attached to their surfaces. Obtained Fe3+-CP-NFs, before investigation of some adsorption parameters for DNA, they were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR). Some attained data from the results of adsorption experiments as follows: maximum DNA adsorption on Fe3+-CP-NFs was found as an excellent value like 845.8 mg/g with an initial concentration of 1.5 mg/mL DNA, at pH 7 and 25 °C. Langmuir and Freundlich adsorption equations were applied to determine which adsorption model was appropriate, and it was seen that Langmuir model was fit with a R2 of 0.9885.
Açıklama
Anahtar Kelimeler
DNA, İMAK, Adsorpsiyon, Nanoflower, Saflaştırma, IMAC, Adsorption, Nanoflower, Separation
