Characterization of intact chitosan obtained from Bradyporus sp. (Orthoptera, Insecta) and its cytotoxicity on human cell line (HEK293)
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Dosyalar
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aksaray Üniversitesi
Erişim Hakkı
Attribution-NonCommercial 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Intact chitosan film was produced from Bradyporus sp. (Orthoptera, Insecta) and characterized by Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM) and elemental analysis. MTT test was performed to determine the cytotoxic effects of chitosan. Chitosan administered at optimal and overdose intervals was applied to HEK293 cells for 24, 48 and 72 hours. In the MTT cytotoxicity test, the IC50 dose of chitosan was 1427 µM at 24 hours, 808.9 µM at 48 hours, and 611.6 µM at 72 hours. Considering all the conducted analyses and cytotoxic experiments, the obtained intact novel chitosan film from the insects could find applications for biotechnological applications.
Açıklama
Anahtar Kelimeler
Chitin, Chitosan, Phytochemistry, HEK293, Cytotoxicity, Bradyporus sp.
Kaynak
Aksaray University Journal of Science and Engineering (ASUJSE)
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
1
