Bir ilçe hastanesinde yara kültürlerinden izole edilen mikroorganizmalar ve antimikrobiyallere duyarlılıkları
[ X ]
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aksaray Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmamızda, hastanemiz mikrobiyoloji laboratuvarına gönderilen yara kültürü örneklerinden izole edilen mikroorganizmaların ve antimikrobiyal duyarlılıklarının retrospektif olarak belirlenmesi amaçlanmıştır. Metot: Mikrobiyoloji laboratuvarına gönderilen yara kültürü örnekleri rutin besiyerlerine ekim yapıldı ve eş zamanlı olarak Gram boyama yapılarak sürüntü örneğinin niteliğine karar verildi. Şuşlarının identifikasyonu konvansiyonel yöntemlerle yapıldı. Antibiyotik duyarlılığının belirlenmesi için disk difüzyon yöntemi kullanıldı. Antibiyotik duyarlılığı The European Committee on Antimicrobial Susceptibility Testing (EUCAST) önerilerine uygun olarak değerlendirildi. Verilerin istatistiksel analizi Statistical Package for Social Scienses istatistik paket programıyla analiz edilmiştir. Bulgular: Kasım 2018–Kasım 2019 tarihleri arasında 139 yara kültürü örneği değerlendirildi. Örneklerin 45’ inde (%32) üreme olmamış ve 20 (%15) örnekte normal cilt flora elemanları üremiştir. Etken bakteri üremesi olan 74 (%53) örnek değerlendirmeye alınmıştır. Beş (%7) örnekte ikili bakteri üremesi saptanmış ve 79 mikroorganizma değerlendirilmiştir. İzole edilen mikroorganizmaların %56’sı Gram pozitif bakteri ve %34’ü Gram negatif bakteriydi. Mikroorganizmaların 41’i (%52) poliklinik hastalarına, 38’i (%48) servis hastalarına ait örneklerden izole edildi. Poliklinik hastalarında en sık karşılaşılan mikroorganizmalar Stafilacoccus aureus, Koagulaz negatif stafilakok (KNS), Klebsiella pneumoniae, Escherichia coli’dir. Yatan hastanın kültürlerinde ise en sık S.aureus, KNS, K.pnemoniae ve Pseudomonas aeruginosa etken olarak üremiştir. Yara kültürlerinde en sık enfeksiyona yol açan mikroorganizma S.aureus suşudur. S.aureus suşlarında yüksek oranda metisilin direnci görüldü. Gram pozitif mikroorganizmalarda penisilin ve eritromisine, Gram negatif mikroorganizmalarda ise ampisilin ve amoksisilin/klavulanata yüksek oranda direnç gözlendi. Sonuç: Yara yeri enfeksiyonlarında etkin tedavi sonucu sağlamak için, yaygın olarak reçete edilen antimikrobiyallerin yerini, izole edilen mikroorganizmanın kültür sonucundaki duyarlı antimikrobiyaller almalıdır.
Purpose: The aim of this study was to determine the microorganisms and their antimicrobial susceptibility isolated from wound culture samples sent to microbiology laboratory retrospectively. Methods: Wound culture samples sent to microbiology laboratory were cultured on routine media and Gram staining was performed simultaneously to determine the quality of the swab sample. Identification of strains was done by conventional methods. Disc diffusion method was used to determine antibiotic susceptibility. Antibiotic susceptibility was assessed in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing. Statistical analysis of the data was analyzed with Statistical Package for Social Scienses. Results: Between November 2018 and November 2019, 139 wound culture specimens were evaluated. 74 (53%) samples with active bacterial growth were evaluated. Five (7%) samples had two bacterial growths and 79 microorganisms were evaluated. 56% of isolated microorganisms were Gram positive bacteria and 34% were Gram negative bacteria. The most common microorganisms in polyclinic patients are Stafilacoccus aureus, Coagulose negative staphylococci (CNS), Klebsiella pneumoniae, Escherichia coli. S.aureus, CNS, K.pnemoniae and Pseudomonas aeruginosa were the most common agents in the cultures of the service patients. The most common infection causing wound cultures is S.aureus strain. Methicillin resistance was high in S.aureus strains. High resistance to ampicillin and amoxicillin / clavulanate was observed in gram negative microorganisms and penicillin and erythromycin in gram positive microorganisms. Conclusions: As a result; In order to achieve effective treatment results in wound infections, commonly prescribed antimicrobials should be replaced by susceptible antimicrobials from the culture of the isolated microorganism.
Purpose: The aim of this study was to determine the microorganisms and their antimicrobial susceptibility isolated from wound culture samples sent to microbiology laboratory retrospectively. Methods: Wound culture samples sent to microbiology laboratory were cultured on routine media and Gram staining was performed simultaneously to determine the quality of the swab sample. Identification of strains was done by conventional methods. Disc diffusion method was used to determine antibiotic susceptibility. Antibiotic susceptibility was assessed in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing. Statistical analysis of the data was analyzed with Statistical Package for Social Scienses. Results: Between November 2018 and November 2019, 139 wound culture specimens were evaluated. 74 (53%) samples with active bacterial growth were evaluated. Five (7%) samples had two bacterial growths and 79 microorganisms were evaluated. 56% of isolated microorganisms were Gram positive bacteria and 34% were Gram negative bacteria. The most common microorganisms in polyclinic patients are Stafilacoccus aureus, Coagulose negative staphylococci (CNS), Klebsiella pneumoniae, Escherichia coli. S.aureus, CNS, K.pnemoniae and Pseudomonas aeruginosa were the most common agents in the cultures of the service patients. The most common infection causing wound cultures is S.aureus strain. Methicillin resistance was high in S.aureus strains. High resistance to ampicillin and amoxicillin / clavulanate was observed in gram negative microorganisms and penicillin and erythromycin in gram positive microorganisms. Conclusions: As a result; In order to achieve effective treatment results in wound infections, commonly prescribed antimicrobials should be replaced by susceptible antimicrobials from the culture of the isolated microorganism.
Açıklama
Anahtar Kelimeler
Yara Kültürü, Antimikrobiyal Duyarlılık, Mikroorganizmalar, Wound Culture, Microorganisms, Antimicrobial Susceptibility
Kaynak
Aksaray Üniversitesi Tıp Bilimleri Dergisi / Aksaray University Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
1
Sayı
2
