Unexpected relationship of extracellular ATP with intracellular Ca concentration in hepG2 cells

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dc.contributor.authorCaner, Armağan
dc.contributor.authorBitgen, Nazmiye
dc.contributor.authorÖnal, Müge Gülcihan
dc.date.accessioned13.07.201910:50:10
dc.date.accessioned2019-07-16T09:16:33Z
dc.date.available13.07.201910:50:10
dc.date.available2019-07-16T09:16:33Z
dc.date.issued2019
dc.departmentTeknik Bilimler Meslek Yüksekokulu
dc.description.abstractThe aim of our study is to examine the effect of exfracellular ATP whether it is correlated with intracellular Ca concentrations ([Ca2+]i) on human liver hepatocellular cells (HepG2). The exfracellular ATP is responsible for regulating both cells signaling and cell functions. ATP maintains these effects through purinergic P2 receptors. The extracellular ATP promotes the release of Ca2+ from the Ca2+ stores to the cytoplasm in the cell and increases [Ca2+] I in the cell. In our study, various concentrations of ATP (10(-3)M-10(-7)M) were applied to HepG2 cells and incubated for 24 hours, 48 hours, and 72 hours. At these concentrations, the proliferation of cells and apoptosis of the cells was examined for 24 hours, 48 hours, and 72 hours. Similarly, cells with different ATP concentrations incubated for 24 hours and 48 hours were loaded with Indo 1FF AM calcium indicator to measure [Ca2+]i. Surprising results were obtained, 10(-6)M-10(-7)M exfracellular ATP was found to be more toxic than 10(-3) M-10(-4) extracellular ATP, (p<0.05). Low concentrations ofATP also reduced [Ca2+]i for 24 hours and 48 hours of incubations (p<0.01). As a result, low concentration exfracellular ATP is more toxic in HepG2 cells. At the same time, the exfracellular ATP correlates with [Ca2+]i.
dc.identifier.endpage100en_US
dc.identifier.issn2319-5886
dc.identifier.issue4en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12451/4616
dc.identifier.volume8en_US
dc.identifier.wosWOS:000470733800012
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSUAMTHI PUBLICATIONS
dc.relation.ispartofINTERNATIONAL JOURNAL OF MEDICAL RESEARCH & HEALTH SCIENCES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectExtracellular ATP
dc.subjectCancer Cells
dc.subjectImaging of Intracellular Ca2+
dc.titleUnexpected relationship of extracellular ATP with intracellular Ca concentration in hepG2 cells
dc.typeArticle

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