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Öğe ADAMTS-13 and HMGB1-induced oxidative stress in Taenia multiceps-infected animals(Nature Research, 2023) Dinçel, Güngör Çağdaş; Yavuz, Orhan; Yıldirım, Serkan; Al-Olayan, Ebtesam M.This study investigated the cytotoxic effects of oxidative stress (OS), high mobility group box 1 (HMGB1), ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs), and neuropathology associated with coenurus cerebralis (Taenia multiceps). ADAMTS-13, HMGB1, glutathione reductase (GR), copper/zinc superoxide dismutase (Cu/Zn SOD), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) expression levels were studied. The study found that ADAMTS-13 (P < 0.005), HMGB1 (P < 0.005), GR (P < 0.005), Cu/Zn SOD (P < 0.005), and 8-OHdG (P < 0.005) levels were significantly higher in T. multiceps (c. cerebralis)-infected animals compared to healthy control animals. This study's most important finding was that HMGB1 up-regulation in neurons, endothelial cells, and glial cells can directly cause brain parenchymal destruction and that HMGB1-mediated oxidative stress plays a crucial role in the neuropathogenesis of coenurosis. The results also showed that increased levels of ADAMTS-13 may play a pivotal role in regulating and protecting the blood–brain barrier integrity and neuroprotection. These findings also suggest that ADAMTS-13 and HMGB1 compete in the prevention or formation of microthrombi, which was regarded as a remarkable finding. ADAMTS-13 and HMGB1 are valuable biomarkers for disease risk assessment, estimating host neuropathy following T. multiceps (c. cerebralis) exposure, and providing a new therapeutic target. This is the first study to show that HMGB1 and ADAMTS-13 are expressed in reactive cells and are associated with neuroimmunopathology in coenurosis.Öğe Bir köşekte (C. dromedarius) nutrisyonel kardiyomiyopati(Selçuk Üniversitesi, 2016) Özdemir, Özgür; Çiftçi, Mustafa Kemal; Hatipoğlu, Fatih; Ortatatlı, Mustafa; Yavuz, Orhan; Kanat, ÖzgürBu çalışmada Türkiye’de ilk kez 1.5 aylık dişi bir köşekte (deve yavrusu) (C. dromedarius) nutrisyonel kardiyomiyopati olgusunun makroskobik ve mikroskobik bulguları tanımlandı. Konya ili, Karatay İlçe Belediyesi, Hayvanat Bahçesi’nde ölü olarak bulunan deve yavrusunun nekropsisinde, sol epikardda daha belirgin olmak üzere her iki ventrikül ile interventriküler septumda solgun, beyazımsı renkte alanlara rastlandı. Akciğerler oldukça hacimli ve koyu kırmızı renkte, trake ve bronşlarda köpüklü sıvı vardı. Mediastinal ve bronşiyal lenf düğümlerinde büyüme, karaciğerde konjesyon ve tiroid bezinde büyüme gözlendi. Mikroskobik incelemede kalp kasında, hiyalin dejenerasyonu, zenker nekrozu ve yaygın kalsifikasyon, akciğerde konjesyon ile alveollerde yaygın ödem vardı. Karaciğerde hafif hiperemi, bağırsaklarda lamina epitelyaliste dejenerasyon ve deskuamasyon, propriyada nötrofil ve eozinofil granülosit infiltrasyonu gözlendi. Tiroid folliküllerinin epitellerinde hiperplaziye bağlı papiller uzantılar dikkati çekti. Bu bulgular ışığında deve yavrusunda ve/ veya annesindeki beslenme yetersizliği sonucunda beyaz kas hastalığı ve buna bağlı akciğerde dolaşım bozukluğu şekillendiği kanaatine varılarak, özellikle hayvanat bahçeleri gibi kapalı ortamlarda bakılan hayvanlara ilave vitamin ve mineral takviyesi yapılmasının uygun olacağı önerilmiştir.Öğe Cardioprotective effects of fetai Icidney-derived mesencliymai stem ceiis on doxorubicin-induced cardiotoxicity in rats(Polskie Towarzystwo Nauk Weterynaryjnych, 2022) Yavuz, Orhan; Boztok Özgermen, Başak; Haydardedeoglu, Ali Evren; Dinçel, Güngör ÇağdaşCardiotoxicity is one of the most common side effects of doxorubicin (DOX), a chemotlierapy drug used in tlie treatment of many carcinomas. In recent years, stem-cell therapies have been successfully used to prevent cardiotoxicity. This study investigated the efficacy of intraperitoneally administered fetal kidney-derived mesenchymal stem cells (FKD-MSCs) in preventing DOX-induced cardiotoxicity in rats. For this purpose, thirty rats were randomly divided into three groups: control, DOX and mesenchymal stem cell (MSG) groups. Adriamycin was injected as a single dose via the tail vein in the DOX and MSG groups in order to induce cardiotoxicity. FKD-MSG was applied to the MSG group by the intraperitoneal route after cardiotoxicity had been established. Then the rats were euthanized, and routine histological procedures were performed on their hearts. H&E and Masson's stains were used for histopathology. Gardiac Troponin-T and I (cTnT, cTnl), Gaspase-3 and BGL-XL antibodies were used for immunohistochemistry. Vacuoles, edema, degeneration and necrosis were observed histopathologically mostly in the DOX group. Lesions in the control and MSG groups were less severe. Fibrosis in the control and MSG groups was milder. cTnT and cTnl immunopositive staining was most commonly seen in the control group, followed by the MSG group. Immunohistochemical staining by Gaspase-3 and BGL-XL showed that their expressions in the MSG group were statistically similar to those in the control group. Accordingly, it was concluded that the intraperitoneal application of MSG had a positive effect on histopathological findings, fibrosis, immunohistochemistry, especially apoptosis, neovascularization, and anti-apoptotic development, whereas troponin levels were not found to be therapeutic.Öğe Cardioprotective effects of fetal kidney-derived mesenchymal stem cells on doxorubicin-induced cardiotoxicity in rats(Polskie Towarzystwo Nauk Weterynaryjnych, 2022) Yavuz, Orhan; Boztok Özgermen, Başak; Haydardedeoglu, Ali Evren; Dinçel, Güngör ÇağdaşCardiotoxicity is one of the most common side effects of doxorubicin (DOX), a chemotherapy drug used in the treatment of many carcinomas. In recent years, stem-cell therapies have been successfully used to prevent cardiotoxicity. This study investigated the efficacy of intraperitoneally administered fetal kidney-derived mesenchymal stem cells (FKD-MSCs) in preventing DOX-induced cardiotoxicity in rats. For this purpose, thirty rats were randomly divided into three groups: control, DOX and mesenchymal stem cell (MSC) groups. Adriamycin was injected as a single dose via the tail vein in the DOX and MSC groups in order to induce cardiotoxicity. FKD-MSC was applied to the MSC group by the intraperitoneal route after cardiotoxicity had been established. Then the rats were euthanized, and routine histological procedures were performed on their hearts. H&E and Masson’s stains were used for histopathology. Cardiac Troponin-T and I (cTnT, cTnI), Caspase-3 and BCL-XL antibodies were used for immunohistochemistry. Vacuoles, edema, degeneration and necrosis were observed histopathologically mostly in the DOX group. Lesions in the control and MSC groups were less severe. Fibrosis in the control and MSC groups was milder. cTnT and cTnI immunopositive staining was most commonly seen in the control group, followed by the MSC group. Immunohistochemical staining by Caspase-3 and BCL-XL showed that their expressions in the MSC group were statistically similar to those in the control group. Accordingly, it was concluded that the intraperitoneal application of MSC had a positive effect on histopathological findings, fibrosis, immunohistochemistry, especially apoptosis, neovascularization, and anti-apoptotic development, whereas troponin levels were not found to be therapeutic.Öğe Comparison of thermographic imaging and other diagnostic techniques in diagnosis of cattle with laminitis(Universidade Federal do Rio Grande do Sul, 2018) Arıcan, Mustafa; Hatipo?lu, Fatih; Erol, Hanifi; Kanat, Özgür; Yavuz, Orhan; Parlak, Kurtuluş; Koç, OsmanBackground: Lameness results in major economic losses on dairy farms. The proportion of lameness in cows are between 4-55%, the origin of 90% lameness are caused by foot diseases. Hoof abnormalities such as laminitis are major contributors to lameness. Aim of the study, compared with thermographic imaging (IRT) and radiographic, magnetic resonans (MR), computer tomography (CT), histopathological analysis on the diagnosis of laminitis. Materials, Methods & Results: Preliminary, the animals (20 Holstein-Fresian) were selected by lameness examination for evaluation of the for any obvious signs of disease. The animals were examined when rising and walking of the free stalls. Walking was very painful and clinical symptoms of general distress become present for laminitic group. The cows were scored on a 1 to 5 scale for their locomotion (1 = normal locomotion; 5 = severely lame). Thermographic examinations were performed the all animals after the routine clinical examination methods. The infrared images were taken from the dorsal view of all hooves to monitor the temperature of coronary band. All images were scanned using a hand-held portable infrared camera (Wahl, Thermal Imager HSI3000 Series), which was calibrated to ambient temperature and absorptive conditions. Incentives radiological examinations, dorso-palmar and latero-medial shooting was done for using of (Regius Model 110 Konica, Minolta) 70 kV and 30 mAs for each claws. MRI images of all isolated digits were performed at the Department of Radiology, by using Siemens Symphony Erlangen, Germany. Continuous series of transversal, sagital and dorsoplantar scan were obtained from all digit. Computer tomography (CT), isolated digits were performed at the Imaging Department of Radiology, by using Siemens Symphony Erlangen, Germany. CT scanner was used to obtain contiguous slices from the region of the proximal sesamoid bones to the toe. Anterior side (laminar and perilaminar tissues) of the claws was uncovered with electric wheel. Tissue samples were taken 10% buffered formaldehyde solution after establishing a routine laboratory procedure following the prepared paraffin blocks 5 micrometers thick sections with hematoxylineosin dye method according to the staining. It was finally examined by light microscopy. The computer soft ware of the infrared thermal camera showed increased local temperature, by 0.5-1.5°C, between the normal and suspected regions for laminitic cases. Clinically, animals suspected of laminitis were showed general stiffness, lameness during walking difficulty. Examination of the claws was demonstrated of rings and plump appearance was evident in paries ungulae. The main damage was seen during trimming of the sole from no hemorrhages or discoloration of control group. The transversal MRI images provided excellent depictions of anatomical structures when compared to their corresponding pictures. Identifiable anatomic structure was labeled on the line drawings of the limb sections and on the corresponding CT images. Twenty out of 30 claws (65%) were shown laminitis statistical with histopathological examination. In histopathological examination; mononuclear cells and neutrophil granulocytes infilrations and edema, haemorrhagia, mild hyperemia in the lamellar region was remarkable in laminitic cases. Discussion: As a results, thermographic examination may have potential as a detection tool for laminitis. MR transversal images provided excellent depiction of anatomical structures and many biometric research in the bovine hoof can be easily investigated. The usefulness of IRT, MRI, CT in evaluating laminitis in the acute patient remains stil open. The use of diagnostic techniques during the initial active phase of laminitis as a means to increase the understanding of the disease and also serves as a justification for the development of an experiment involving live cows induces laminitis.Öğe Evaluation of fetal heart serum amyloid a concentrations in infectious cattle abortion cases(Elsevier Ltd, 2022) Aras, Zeki; Yavuz, OrhanSerum amyloid A (SAA) concentrations have been investigated in diseases of human and domestic animals and increased SAA levels have been reported in infectious diseases. In the present study, we determined the fetal heart blood SAA concentrations in aborted bovine fetuses and investigated the relationship between the level of SAA and causative infectious agents. A total of 46 heart blood samples were collected from aborted bovine fetuses between July 2018 and July 2019 and were assigned to two groups according to microbiological, pathological and molecular results. Group 1: An infectious disease was diagnosed by detecting a microorganism (21 cases); Group 2: An infectious or inflammatory disease was not detected (25 cases). The fetal heart blood SAA concentrations were measured by commercial ELISA test. Serum amyloid A concentrations in aborted bovine fetuses were elevated from 6.1 to ?40 mg/L in 17 of 21 cases in group 1. In group 2, SAA concentrations were less than 2.5 mg/L in 23 of 25 cases. This difference was statistically significant between group 1 and group 2. These findings suggest that SAA concentrations in fetal heart blood from bovine fetuses is potentially a novel marker for distinguishing between infectious and non-infectious bovine abortion cases.Öğe Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia(Polish Journal of Veterinary Sciences, 2020) Yavuz, Orhan; Dinçel, Güngör ÇağdaşThis study aimed to determine the expression of some cytokines, such as cyclooxygenase-2 (COX-2), High Mobility Group Box-1 (HMGB-1) and CD68, in the lung tissue of sheep with fibrinous bronchopneumonia by immunohistochemical methods. Forty sheep which had suffered from respiratory problems were brought for necropsy to the Faculty of Veterinary Medicine, Department of Pathology, Aksaray University, between November 2014 and December 2017. After necropsy, lung tissues grossly diagnosed with fibrinous bronchopneumonia were processed histologically, and stained histopathologically and immunohistochemically. Bacteriological culture was also applied to the lung tissues to isolate the agents. In histopathological examinations, congestion, red consolidation and grey consolidation stages were detected in the lung tissues. In such cases, we observed fibrin masses accumulated in some alveolar lumens, as well as inflammatory cell infiltrations of various extent in alveolar and bronchiolar lumens. In the interalveolar septum, a thickening was observed due to a fibrin mass and thrombotic vessels. Immunohistochemically, it was determined that COX-2 and HMGB-1 cytokines showed positive reactions, especially in bronchial, bronchiolar and alveolar epithelia, as well as goblet cells and macrophages. CD68 was found to be expressed in alveolar macrophages. COX-2 and HMGB-1, which have been implicated in the inflammatory response, were also shown to be expressed in fibrinous bronchopneumonia in sheep for the first time. Thus, these cytokines are thought to play a role in the pathogenesis of the disease. Moreover, their increased expression suggests that it may be helpful in the diagnosis of the disease.Öğe Histopathology changes in the rainbow trout (Onchorhyncus mykiss) consuming boric acid supplemented fish fodder(Elsevier GmbH, 2020) Öz, Mustafa; Yavuz, Orhan; Bölükbaş, FerhanObjective: Even though boron (B), as a trace micronutrient, occurs in natural waters and organisms, its high concentration could cause harmful and even toxic for organisms. The aim of present study was to investigate the effects of boric acid (BA) added to feed (0.01%, 0.05%, 0.10%, and 0.20% of B in feed) on Rainbow Trout (Oncorhynchus mykiss) by histopathological methods and compared to the control feed as without B. Methodology: At the end of the feeding, after weighing the live weight of 9 rainbow trout from each group, tissue fragments were taken from the liver, gill, kidney, skeletal muscle, spleen and brains of fish, which systemic necropsies was done, and were fixed in 10 % buffered formalin solution. Results: Histopathological examinations revealed degenerative and necrotic changes in the liver, gill, kidney, skeletal muscle, spleen and brain. Hydropic and vacuolar degenerations in liver parenchyma, lamellar edema in the gills, hyaline accumulation in the kidneys, degenerations in the muscles, necrosis in the spleen and hyperemia in the brain were observed in all groups except control group. It was observed that the destruction of boric acid on fish increased depending on the amount of boron supplemented to the feed, and the most effect was in the group fed with 0.20 % boron supplemented feed. Conclusions: The findings aside from causing pathological changes in all organs in terms of histopathological findings of Boric Acid (BA), the most severe lesions were observed in the liver.Öğe Immunohistochemical and immunocytochemical findings associated with Marek's disease virus in naturally infected laying hens(Taylor & Francis, 2017) Yavuz, Orhan; Erer, H.We compared immunohistochemical (IHC) staining of tissue sections of liver, kidney, spleen, lung, proventriculus, sciatic nerve, bursa of Fabricius, brain, heart, intestine and skin; immunocytochemical (ICC) staining of peripheral blood samples and touch preparations of liver, spleen and kidney of laying hens naturally infected with Marek's disease (MD) virus. We used one hundred and fifty 5-17-week-old commercial hens. IHC and ICC staining were performed using polymer-based techniques. IHC staining exhibited mostly free immunopositive reactions in tumor cells and in the cytoplasm of the parenchymal cells of liver, kidney, spleen and bursa of Fabricius. In the sciatic nerve, severe reactions were observed in the cytoplasm of plasma and MD cells in the lymphoproliferative areas. Pronounced staining was found in the lymphoid cells in the medulla of intrafollicular regions in the bursa of Fabricius. Although immunostaining was observed in the liver and spleen touch preparations, there was no staining in the kidneys and peripheral blood cell samples. The presence of virus in the tissue and peripheral blood samples and in touch preparations was compared immunohistochemically and immunocytochemically. IHC and ICC techniques were helpful for diagnosis of MD. Peripheral blood samples are inappropriate for field conditions and natural infections.Öğe Immunohistochemical studies on infectious laryngotracheitis in the respiratory tract lesions in naturally infected laying hens(Kafkas Üniversitesi, 2018) Terzi, Funda; Aras, Zeki; Özdemir, Özgür; Yavuz, OrhanIn this study, naturally infected by Gallid Herpesvirus type-1 in laying hens to be diagnosed by pathological and PCR methods. Sixty pieces of hens were collected in coops from Central Anatolia region. After necropsy, routine pathological processes were applied to the trachea/ larynx, sinuses, lungs and air sacs. All organs were also stained by immunoperoxidase method, and PCR methods were applied to formalin fixed paraffin embedded (FFPE) tissues. Immunohistochemically, the positivities were seen in trachea/larynx (78.3%), sinuses (61.6%), lungs (45%) and air sacs (50%). Positive reactions were observed, in mucous and gland epithelia especially located at intracytoplasmic and rarely intranuclear. PCR positivity was observed in the trachea/larynx in 15 (25%) cases, in infraorbital sinus in 11 (18.3%) cases, in lungs in 8 (13.3%) cases and in air sacs in 6 (10%) cases following the tests performed. Following these results, it is easily concluded that histopathology and immunoperoxidase method can usable for diagnosing of the ILT. However, PCR results made by FFPE tissues showed that this method is not adequate to diagnose the ILT alone.Öğe Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats(Chartered Inst. of Building Services Engineers, 2022) Boztok Özgermen, Başak; Bulut, Gaye; Alparslan Pınarlı, Ferda; Gültekin, Salih Sinan; Özen, Doğukan; Yavuz, Orhan; Haydardedeoğlu, Ali EvrenThe potential protective effects of mesenchymal stem cells (MSCs) on some kidney diseases have been reported. However, the effect of the fetal kidney–derived (FKD)MSCs on doxorubicin-induced nephropathy has not been studied yet. This study aimed to treat rats with doxorubicin-induced kidney injuries by transplantation of –FKD-MSCs. Twenty-four Sprague-Dawley rats were divided into three groups as control, doxorubicin nephropathy (Sham), and doxorubicin + MSC treated group. Serum biochemistry analysis was performed at the beginning and the end of the study. Functional changes in kidneys were evaluated by scintigraphy. In the doxorubicin nephropathy group, histopathological findings such as mesangial cell proliferation, tubular cast, and glomerular hypertrophy were observed, whereas in the MSC group these findings were significantly reduced. CD133 and CD24 positive immunoreactions were the most severe and frequently observed in the MSC group. While positive staining was detected in the tubular epithelium, there was no immunostaining observed in the glomerulus. The results showed that both functional and histological improvements were achieved in the MSC group compared to the Sham group. In conclusion, transplantation of fetal kidney - derived MSCs into patients with renal damage is thought to contribute to the healing of the renal tissue.Öğe Investigations of pathological immunohistochemical and immunocytochemical findings in natural Infection with mycoplasma gallisepticum in laying hens(University Fed Rio Grande Do Sul, 2019) Özdemir, Özgür; Yavuz, Orhan; Erer, Hudaverdi; Sayın, ZaferBackground: Mycoplasmosis is an infectious disease caused by Mycoplasma gallisepticum (MG), usually seen in the respiratory system of chickens, chick and turkeys, that causing great economic loss. The disease is characterized by respiratory system lesions such as sinusitis, tracheitis, airsacculitis, pneumonia and other symptoms such as loss of yield, arthritis, tenosynovitis. In this study, it was aimed to investigate diagnose of the disease by pathologic and molecular techniques in hens that naturally infected with MG as well as the usability of immunocytochemical (ICC) method in diagnose of the disease. Materials, Methods & Results: For this purpose, 98 hens were collected from 10 different coops that serologically positive. After necropsy, routine pathological procedures were performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Scraping samples taken from lungs and tracheas were evaluated by ICC. Immunohistochemical (IHC) staining was performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Indirect immunoperoxidase method was applied in the both IHC and ICC staining. Rabbit polyclonal anti MG antibody was used as primer antibody in the IHC and ICC staining. Additionally, culture and PCR techniques were applied to tracheas of all hens for MG. The GPO3 and MGSO genes were made for PCR analysis. In the tracheal examinations, 23 cases were positive for PCR, 17 cases ICC positive, 16 cases IHC positive and 10 culture samples found positive. All of culture positive cases were also positive for other three methods. When findings in all organs were evaluated, in 37 cases were detected positive by IHC (38%) and 23 cases were positive by ICC (23.5%). In the IHC positive cases, the first order was trachea in 16 cases followed by in 11 cases in sinus, in 8 cases in lung, in 6 cases air sac and 4 cases in nose, respectively. In 8 cases, IHC positivity was found in at least two organs. IHC positivity was detected in the nose, sinus and tracheal epithelia as well as in the macrophages within subepithelial lymphoid infiltration, vascular walls and endothelium. As the disease became chronic, it was found that the agents were seen more in the lymphoid tissue than the epithelium. In ICC staining positivity was found in 17 cases in the trachea and 11 cases in the lung. There were only 5 cases positive by ICC in both organs. Discussion: Clinical and pathological findings as well as serological, microbiological, molecular techniques and immunohistochemical methods are to be important methods in the diagnosis of the disease. While the culture results are shown as the gold standard in diagnosis of the disease, it is possible to obtain the results in the earliest 7-10 days in cultures and at least 20 days must be passed in order to say a cultural negative. In addition, in the field studies, it mentioned the use of vaccines, antibiotics and protective drugs affected the results of microbiology and serology; the importance of using techniques such as IHC and PCR for the diagnosis of the causative agents. The results of the present study indicate that the most important organ in the diagnosis of the disease is the trachea, and the most effective method is PCR followed by IHC and ICC methods. It was concluded that the results of ICC staining close to IHC staining, and ICC could be used for diagnostic purposes in positive reactions obtained from the tracheas or the other organs.Öğe Macroanatomical and histological study of caecum of the guinea fowl (Numida meleagris) using light and scanning electron microscopy(Agricultural Research Communication Centre, 2018) İlgün, Ramazan; Gür, Fatih Mehmet; Bölükbaş, Ferhan; Yavuz, OrhanThe aim of this study was to investigate the macroanatomy and histology of the caecum in guinea fowl using light and scanning electron microscopy. Six adult male and six adult female guinea fowl were used as the study material. The cavum abdominis of the animals was opened under anaesthesia, and the caeca uncovered. The height and thickness of the villi were smaller in the apex and corpus caeca than in the basis caeci. The SEM image of the guinea fowl caeca showed that the villi send finger like extensions into the lumen, and the height of the villi towards the basis caeca increases and exhibits a tight structure. Thus, in this study, the anatomy and histology of guinea fowl caeca were examined in detail using light and scanning electron microscopy, and the similarities and differences with the caeca of other poultry species were investigated.Öğe Mesenchymal stem cells reduce left ventricular mass in rats with doxorubicin-induced cardiomyopathy(Sociedad Chilena de Anatomía, 2018) Haydardedeoglu, Ali Evren; Boztok Özgermen, Deva Başak; Yavuz, OrhanDoxorubicin is a drug that used by a majority in the treatment of carcinomas. The most obvious known side effect is cardiomyopathy. Many studies have been carried out to eliminate side effects of the doxorubicin, and stem cell studies have been added in recent years. In this study, it was aimed to investigate fetal-derived mesenchymal stem cells (F-MSCs) treatment of doxorubicin-induced cardiomyopathy by morphological methods. A total of 24 rats which were divided into three separate groups (Control, sham, treatment), each consisting of 8 male rats were used. In sham and treatment group, Adriamycin was administered in a single dose by tail injection to perform cardiotoxicity. In the treatment group, F-MSCs were intra-peritoneally administrated. Then, rats were euthanized and their hearts were photographed at the level of papillary muscle. and thickness, diameters and surface area levels were measured. Left ventricular mass (LVM) and left ventricular mass index (LVMI) were calculated after measurement. The sham group, LVM and LVMI levels were found to significantly lower (p<0.05) than control and treatment group. In the one hand, LVMI levels of rats in treatment group was statistically similar (p>0.05) to control group. Similarly, LVM levels of control and treatment groups were close to each other while this level of sham group was lower. It has been shown that F-MSC administrations in rats with doxorubicin-induced cardiomyopathy have adverse effect on LVM and LVMI values. In addition, the intra-peritoneal MSC administrations may be an alternative to other injection routes such as intra-venous and intra-cardiac administrations.Öğe Occurrence of infectious laryngotracheitis outbreaks in commercial layer hens detected by ELISA(Taylor and Francis Inc., 2018) Aras, Zeki; Yavuz, Orhan; Sanioğlu Gölen, GökçenurInfectious laryngotracheitis (ILT) is an acute respiratory disease of chickens and a cause of great economic loss in commercial layers. The aims of this study were to investigate the prevalence of ILT in the field outbreaks and to compare the characteristics of ILT-infected and free flocks of commercial layers. A total of 625 blood serum samples were collected from 25 different layer flocks. The presence of antibodies against infectious laryngotracheitis virus (ILTV) in each sample was determined by ELISA. Of the 625 serum samples, 266 (42.56%) were found to be positive for ILTV antibodies. A total of 16 (64%) flocks were detected ILT positive by ELISA method. The mortality of infected flocks was statistically higher (P < 0.05) than uninfected flocks. The egg production of positive flocks was lower than that of the free flocks, but this difference was not statistically significant. The average live weight of hens in infected flocks was lower (P > 0.05) than hens in free flocks. In conclusion, the results of this study indicated a high prevalence of ILT infection in the commercial layer flocks in Konya region, Turkey. In outbreaks, ILT significantly increased the mortality rate and decreased the average live weight in layer hens. © 2018, © 2018 Taylor & Francis.Öğe Role of glial fibrillary acidic protein (GFAP) and neurofilament (NF) expression in the pathophysiology of canine distemper encephalomyelitis(Ebubekir CEYLAN, 2023) Dinçel, Güngör Çağdaş; Yavuz, Orhan; Yıldırım, SerkanCanine distemper virus (CDV), a member of the genus Morbillivirus of the family Paramyxoviridae, is the causative agent of canine distemper, a fatal and highly contagious disease that affects dogs and other carnivores. This study aimed to investigate whether there is a correlation between glial fibrillary acidic protein (GFAP) and neurofilament (NF) expression in canine distemper encephalomyelitis (CDE) and the severe neuropathology that occurs. Materials and Methods: GFAP and NF expression levels in the brain tissue of 13 dogs diagnosed with CDE were investigated by immunohistochemical method. Results: The results of the study revealed that GFAP (P < 0.005) and NF (P < 0.005) expression levels in brain tissue were significantly increased in CDV-infected dogs compared to healthy, uninfected dogs. GFAP expression was mainly observed in endothelial cells and astrocytes, whereas NF expression was mainly found in neurons. In addition, it was found that the expression of both GFAP and NF was more pronounced in the areas with the most severe neuropathological findings. Conclusions: This study demonstrated pathological astrocyte reactivation and neuronal degeneration at the molecular level. These findings provide information about the stage of the disease. This study clearly demonstrated that detailed information about the prognosis of the disease can be obtained from GFAP and NF expression. Since GFAP/NF levels provide information about the severity of the disease, they can be used clinically. Therefore, further research into the involvement of GFAP and NF expression in the pathophysiology of CDE has great potential to improve our understanding of this complex neurological disorder.Öğe The effect of Pneumonic Pasteurellosis on Apoptosis and Nitric Oxide Synthase in the Lungs in Calves(TURJAF, 2020) Yavuz, Orhan; Dinçel, Güngör ÇağdaşPneumonic Pasteurellosis (PP) is an infectious disease caused by Pasteurella multocida and Mannheimia haemolytica, mostly observed in cattle, sheep and calves. PP is characterized by fibrinous bronchopneumonia and pleuritis in the lungs. In this study, it was aimed to determine Caspase-3, Caspase-9, inducible nitric oxide synthase and neuronal nitric oxide synthase expressions by immunohistochemical methods in the lungs suffered from PP. For this purpose, twenty lung tissues were collected from calves with PP. For the Control Group, ten lungs of calves were collected from Aksaray Slaughterhouse. After necropsies of calves were confirmed to be PP by bacteriological examinations. Then the routine histological process was performed to tissues, and stained by Hematoxylin & Eosin for histopathology, and Caspase-3, Caspase-9, inducible nitric oxide synthase and neuronal nitric oxide synthase antibody staining for immunohistochemistry. The immunohistochemical findings indicated that Caspase-3, Caspase-9, inducible nitric oxide synthase and neuronal nitric oxide synthase positive reactions were seen in alveolar, bronchial and bronchiolar epithelia, and desquamated inflammatory cells in the lumens. In addition, the peripheral neural extensions were immunopositive for neuronal nitric oxide synthase and vascular endothelial cell were positive for inducible nitric oxide synthase. The findings can contribute to a better understanding of expressions of molecules such as Caspase and nitric oxide synthase. These results show that apoptosis and nitric oxide synthase expressions have triggered by airway epithelia and inflammatory cells in the lungs with Pneumonic Pasteurellosis in calves.Öğe The investigation of cell adhesion molecules in the lung tissues of cattle with cystic echinococcosis(Veteriner Kontrol Merkez Araştırma Enstitüsü, 2020) Yavuz, Orhan; Dinçel, Güngör Çağdaş; Gökpınar, Çağdaş; Hyadardedeoğlu, Ali EvrenCystic echinococcosis is a zoonotic disease with worldwide distribution caused by Echinococcus granulosus, represents a substantial global health problem. Hydatid cyst (Echinococcus) has a remarkable negative effect on the health of people and the economic development of the country. The objective of this study was to investigate the CD68, nicotinamide nucleotide adenylyltransferase 3 (NMNAT 3), Neuregulin 1 (NRG1) and Neuregulin 2 (NRG2) expressions in bovine lungs infected with E. granulosus and to identify whether they have any correlation with pulmonary pathology. For this purpose, 30 bovine lung tissues were used between January 2016 and December 2016 collected in Kırıkkale slaughterhouse. In histopathologic examinations, proliferation of fibrous connective tissue and infiltration of mononuclear cells were detected in the lung tissues of the bovine. Most of the cysts were seen to be quite thick capsule. There was also a cellular line rich in abundant fibroblasts and mononuclear cells. The cyst wall was found to be an eosinophilic laminar structure. There was infiltration with lymphocytes and macrophages, especially eosinophils and giant cells. Immunohistochemically, CD68 positivity was seen around the bronchi, bronchioles and cystic matter. However; NMNAT 3, NRG1 and NRG2 showed no positive reactions in macrophages, bronchi, bronchioles and alveolar epithelium. These results indicate that NMNAT 3, NRG1 and NRG2 pathways were not used in pulmonary pathology. Therefore, it is the most important result of the study that the adhesion molecules in pulmonary pathology are not originating from NMNAT 3, NRG1 and NRG2.Öğe The preventive effects of different doses of Glucomannan on experimental aflatoxicosis in Japanese quails(Fundacao APINCO de Ciencia e Tecnologia Avicolas, 2017) Yavuz, Orhan; Özdemir, Özgür; Ortatatlı, Mustafa; Atalay, B.; Hatipo?lu, Fatih; Terzi, FundaThis experimental study was performed to investigate whether there is a protective effect of different doses of Glucomannan using against aflatoxicosis in Japanese quail, and pathological changes and relative organ weights were compared. In the experiment, 60 one-day old male Japanese quails were used as divided into six different groups. Experimental groups were designated as Control(C), aflatoxin(A), glucomannan(GM), 2-fold dose of glucomannan(2GM), aflatoxin+glucomannan(A+GM) and aflatoxin+2-fold dose of glucomannan(A+2GM). While control group quails fed the standard ration as ad libitum, other groups were fed with the administrations additionally to standard diet respectively; 2mg/kg of aflatoxin to group A, 1g/kg of glucomannan to group GM, 2g/kg of glucomannan to group 2GM, 2mg/kg of aflatoxin and 1g/kg glucomannan to group A+GM, 2mg/kg of aflatoxin and 2g/ kg glucomannan to group A+2GM. All quails were euthanized at day 21 of the study and organs, (liver, spleen, kidney, thymus and bursa of Fabricius) were removed, weighed and subjected to routine histopathological procedures. Although any important macroscopic changes were not observed in the C, GM and 2GM groups, significant pathological changes were found in the groups of A, A+GM and A+2GM. In the A+GM group, the partial reduction in the severity of microscopic lesions were seen in liver, bursa of Fabricius, thymus and spleen, however a significant reduction in severity of lesions was noticed in A+2GM group. As a result of the study, 2g/kg of glucomannan has been found pathologically to be more effective than 1g/kg glucomannan in terms of the protection against aflatoxicosis by giving orally. © 2017, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.
