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    Cryoprotective effect of vaccenic acid supplemented in bull semen extender
    (Scientific and Technological Research Council of Turkey, 2022) Avdatek, Fatih; Taşdemir, Umut; İnanç, Muhammed Enes; Yeni, Deniz; Gülhan, Mehmet Fuat; Güngör, Şükrü; Türkmen, Ruhi; Tuncer, Purhan Barbaros
    The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 mu g / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 degrees C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 group (p < 0.05). Except for VA200, the glutathione level was greater in the treatment groups in comparison to the control group (p < 0.05), while the lowest malondialdehyde was determined in VA25. In conclusion, VA supplemented with TRIS-based extender at the concentration of 25 mu g / mL improves the progressive and total motility properties of spermatozoa, reduces the abnormal spermatozoa rate, and has a positive effect on chromatin integrity.
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    Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages
    (Elsevıer Scıence Bv, 2018) İnanç, Muhammed Enes; Tekin, Koray; Olgaç, Kemal Tuna; Yılmaz, Burak; Çil, Beste; Taşdemir, Umut; Tuncer, Purhan Barbaros; Büyükleblebici Serhat
    The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): <= 3 years, n: 20; middle age (M): 4-6 years, n: 20; old age (O): >= 7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5 mg/120 x 10(6) CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48 h. Later, the frozen straws were thawed in a water bath for spermatological evaluation. Significant differences were observed between different age groups in terms of the spermatological parameters (p < 0.05). The evidence suggests that increasing age is associated with poor in-vitro spermatological parameters and CLC was able to protect the acrosome integrity from cryo-damage during the freeze-thawing process. Better semen freezability characteristics were obtained at young ages, considering the overall parameters.
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    Genomewide association of male reproductive traits in Aksaray Malakli dogs
    (Wiley, 2018) İnanç, Muhammed Enes; Tekin, Koray; Akkurt, Mustafa Yenal; Olgaç, Kemal Tuna; Yılmaz, Burak; Çil, Beste; Kızılaslan, Mehmet; Taşdemir, Umut; Tuncer, Purhan Barbaros; Büyükleblebici, Serhat; Uysal, Ongun; Çınar Kul, Bengi
    Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) genotype of the investigated characteristics and reproductive loci. A total of 46 male dogs are divided into three age groups (I <= 3 years, n = 19; II 4-6 years; n = 19, and III >= 7 years, n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The Affymetrix Canine 127 K SNP genotyping array v2 (Affymetrix Inc., California, USA) was used for SNP genotyping. In the primary results, the scrotal circumference was found to be higher in group II compared to other groups (p < 0.05) and the lowest total abnormal spermatozoa rate was found in group I (p < 0.05). The normal spermatozoa rate was found to be significantly above the threshold in relation to the SNP in chromosome 17. In conclusion, this study represents an exciting first step towards SNP association with dog semen spermatological parameters. Future studies might be undertaken to evaluate this SNP region for gene-knockout and expression analysis and for fine mapping to validate and/or discover the exact position of the effect region.
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    The effect of green tea extract supplementation in bull semen cryopreservation
    (Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2019) İnanç, Muhammed Enes; Çil, Beste; Yeni, Deniz; Avdatek, Fatih; Orakçı, Durmuş; Tuncer, Purhan Barbaros; Türkmen, Ruhi; Taşdemir, Umut
    This study aims to investigate the effect of catechin (CT), green tea extract, as a supplement to Tris extender on semen quality parameters in frozen-thawed of bull sperm. Ejaculates were taken with artificial vagina from Holstein bulls and divided equal five aliquots, diluted to containing different amounts of CT (5, 10, 25 and 50 mu g/mL) and no-additive (control). All samples were equilibrated at 4 degrees C for 4 h and were frozen using a digital freezing machine. Post-thawed sperm motility and kinetic parameters were determined using the sperm analyser system. Spermatozoa DNA integrity was evaluated with the single cell gel electrophoresis, abnormal spermatozoa rate was evaluated by fluid fixation test and lipid peroxidation status was evaluated calorimetrically. CT supplementation did not improve motility and kinetic parameters. However, the higher morphological integrity was detected in CT10, 25 and 50 groups compared to control (P<0.05). Regarding chromatin integrity, positive effects of catechin were observed in the treatment groups while in CT 50 group adverse effects were found (P<0.05). Although there was no improvement in malondialdehyde levels, the highest total antioxidant activity was seen in the CT50 group (P<0.05). In conclusion, CT supplementation could be used the protection of morphological and DNA integrity from cryodamage and it has increased the total antioxidant activity depending of the dose in bull semen.