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Öğe Antimicrobial and antioxidant activities of various extracts of endemic Crepis macropus Boiss & Heldr (Asteraceae)(Current Biology, 2011) Aşan Özüsağlam, Meltem; Karakoca, Kübra; Erzengin, Mahmut[Abstract Not Available]Öğe Antimicrobial and antioxidant activity of various solvent extracts of Salsola stenoptera wagenitz and Petrosimonia nigdeensis aellen (Chenopodiaceae) plants(Chiang Mai University, 2015) Aşan Özüsağlam, Meltem; Erzengin, Mahmut; Darılmaz, Derya Önal; Erkul, Seher Karaman; Tekşen, Mehtap; Karakoca, KübraThis study was conducted to examine the in vitro antimicrobial and antioxidant activities of various extracts (ethanol, methanol, n-hexane, dicholoromethane and water) prepared from the stem and leaf+fruit of Salsola stenoptera Wagenitz and Petrosimonia nigdeensis Aellen (Chenopodiaceae) plants. The antimicrobial activities of the plant extracts were tested according to agar well diffusion and microdilution broth methods. The extracts showed a strong antimicrobial activity and a broad antimicrobial spectrum for all tested microorganisms. Minimal bactericidal (MBC) or fungicidal (MFC) concentrations for microorganisms which were sensitive to the stem and fruit+leaf extracts of S. stenoptera and P. nigdeensis in the agar well diffusion assay were in the range of 5.63-180.00 mg/mL and 11.25-180.00 mg/mL, respectively. The extracts were also subjected to screening for their possible antioxidant activity with scavenging activity of DPPH radicals, hydrogen peroxide scavenging activity and chelating ability on ferrous ions. All tested extracts showed varying degrees of efficacy in each assay in a dose-dependent manner. In general, polar extracts exhibited stronger activities than non-polar extracts. The ethanol and n-hexane extracts of S. stenoptera and P. nigdeensis stem showed the highest scavenging abilitiy on DPPH radicals with the IC50 values of 29.84 µg/mL and 83.56 µg/mL, respectively. The total phenolic and flavonoid contents were also determined. The highest gallic acid equivalents (GAE) and quercetin equivalents (QE) values, for both plants, were 44.78 µg GAE/mg and 66.69 µg QE/mg, respectively. The results indicate that both plant species may have health benefits as sources of natural antioxidants and antimicrobial agents for use in functional foods and/or in pharmaceutical industry. © 2015, Chiang Mai University. All rights reserved.Öğe Antioxidative, antimicrobial and cytotoxic properties of Isatis floribunda Boiss. ex Bornm. extracts(2013) Karakoca, Kübra; Aşan Özüsağlam, Meltem; Çakmak, Yavuz Selim; Erkul, Seher KaramanThe Isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of Anatolian-Turkey. In the present study, the antioxidant activities of Isatis floribunda Boiss. ex Bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. The total phenolic compounds and flavonoids were also examined for the extracts. The antimicrobial activities of the extracts were investigated by using the disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The phenolic composition of I. floribunda root methanolic extract was analyzed by high performance liquid chromatography (HPLC). The major component in the extract was cholorogenic acid (1980.20 µg/g). The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.Öğe Evaluation of biological activity and antioxidant capacity of Turkish licorice root extracts(2014) Aşan Özüsağlam, Meltem; Karakoca, KübraGlycyrrhiza glabra L. (Licorice) is a well-known medicinal herb that grows in various parts of the world. In the current study, the antimicrobial activities of n-hexane and dichloromethane extracts from G. glabra root were investigated by using the disc-diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The antioxidant activities of the extracts were determined with total antioxidant activity, DPPH activity, ferric ion reducing power and CUPRAC assay. The total phenolic compounds and flavonoids were also studied for the extracts. In antimicrobial screening, G. glabra root extracts showed potent antimicrobial activity against all the tested clinical and food-borne pathogenic microorganisms. Streptococcus agalactiae exhibited a higher sensitivity against the dichloromethane extract with the highest zone diameter (18.21 mm) and the lowest MBC value (1.41 mg/ml) of all the fish pathogens. On the other hand, the free radical scavenging activities of n-hexane and dichloromethane extracts were found as IC50 579.98 µg/ml and 111.49 µg/ml, respectively. The results showed that the root dichloromethane extract has a stronger antioxidant capacity compared with n-hexane extract. The findings indicated the potential use of the n-hexane and dichloromethane extracts from G. glabra root as biopreservatives as they demonstrated high antimicrobial and antioxidant activities. © 2014 University of Bucharest.Öğe Phenolic Compounds, biological and antioxidant activities of onobrychis armena Boiss. & Huet flower and root extracts(Chiang Mai University, 2015) Karakoca, Kübra; Aşan Özüsağlam, Meltem; Çakmak, Yavuz Selim; Tekşen, MehtapOnobrychis armena Boiss.&Huet (Fabaceae) is a plant species that is endemic to Turkey and grows in Aksaray. The aim of the present study is to examine antimicrobial and antioxidant activities of crude extracts of O. armena flower and root. The antimicrobial activities of the extracts were investigated by using disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The results showed that all the extracts exhibited varying degrees of antimicrobial activity on the microorganisms tested. The antioxidant activity was determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. In addition, the total phenolic compounds and flavonoids were measured in the extracts. The phenolic composition of the methanolic root extract was analyzed by HPLC. The HPLC analysis showed that the major component was rutin (428.80 µg/g). Cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that flower and root extracts of O. armena may be a potential source of natural antimicrobial and antioxidants and could be used as a natural source in food industry, drug discovery, clinical and food chemistry, and antimicrobial agents against human and fish pathogens. © 2015 Chiang Mai University. All rights reserved.
