Yazar "Jawad, Marwa H." seçeneğine göre listele

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    Gold nanoparticles loaded TNF-? and CALNN peptide as a drug delivery system and promising therapeutic agent for breast cancer cells
    (Taylor and Francis Ltd., 2022) Jabir, Majid S.; Abood, Noor A.; Jawad, Marwa H.; Öztürk, Kamile; Kadhim, Haider; Albukhaty, Salim; Al-Shammari, Ahmed; AlMalki, Faizah A.; Albaqami, Jawaher; Sulaiman, Ghassan M.
    We investigated the anti-cancer properties of gold nanoparticles loaded TNF- and CALNN peptides, which we proposed as a potential drug delivery system using in vitro and in vivo models. The binding of GNPs-TNF- and GNPs-TNF-CALNN was characterized using a UV, ELISA and SEM analysis. The outcomes demonstrated that a novel drug delivery system had an anti-proliferative activity against breast cancer cell lines through a mechanism of apoptosis induction. In vivo model involved studying the cytotoxic influence of a drug delivery system GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN when applied to the transplanted AN-3 cell line. tumor sections were examined using microarray. In-vivo studies demonstrated that GNPs alone had less of a growth inhibitory effect on tumors implanted in mice when compared to GNPs-TNF–CALNN combined therapy. The cytotoxic assay showed that GNPs, GNPs-TNF-? and GNPs-TNF-?-CALNN exhibit selective toxicity towards cancer cells, inducing cell apoptosis through activation of caspase-3 and 7, p53 protein.
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    Induction of apoptosis and autophagy via regulation of AKT and JNK mitogen-activated protein kinase pathways in breast cancer cell lines exposed to gold nanoparticles loaded with TNF-? and combined with doxorubicin
    (Walter de Gruyter GmbH, 2023) Jawad, Marwa H.; Jabir, Majid S.; Öztürk, Kamile; Sulaiman, Ghassan M.; Abomughaid, Mosleh M.; Albukhaty, Salim; Al-Kuraishy, Hayder M.; Al-Gareeb, Ali I.; Al-Azzawi, Waleed K.; Najm, Mazin A. A.; Jawad, Sabrean F.
    Gold nanoparticles (GNPs) tagged with peptides are pioneers in bioengineered cancer therapy. The aim of the current work was to elucidate the potential anticancer interactions between doxorubicin and GNPs loaded with tumor necrosis factor-alpha (TNF-?). To investigate whether GNPs loaded with TNF and doxorubicin could stimulate autophagy and apoptosis in breast cancer cells. Two human breast cancer cell lines, MCF-7 and AMJ-13, as well as different apoptotic and autophagy markers, were used. In both cell types, treatment with TNF-loaded GNPs in conjunction with doxorubicin increased the production of apoptotic proteins including Bad, caspase-3, caspase-7, and p53 with upregulation of the LC3-II and Beclin1 proteins. In addition, the findings showed that the mitogen-activated protein kinase signaling pathway was dramatically affected by the GNPs loaded with TNF-? and combined with doxorubicin. This had the effect of decreasing p-AKT while simultaneously increasing p-JNK1/2. The findings demonstrated that GNPs loaded with TNF-? and combined with doxorubicin can induce both autophagy and apoptosis in breast cancer cells. These results suggest that TNF- and doxorubicin-loaded GNPs provide a therapeutic option as a nanomedicine to inhibit the proliferation of breast cancer.